Homework Answers
Answer: If a PCR is performed of the given genotype and product is run on the Gel, we expect to see 9 bands on the gel (or capillary electroporosis).
Add Answer to:
• What would you expect to see on a gel if PCR is performed for a...
What would you expect to see on a gel if PCR is performed for a microsatellite...
What would you expect to see on a gel if PCR is performed for a microsatellite in a person with a genotype indicated by: (Allele 1: 3 TAGA repeats) (Allele 2: 6 TAGA repeats) ---TAGATAGATAGA------ ---TAGATAGATAGA TAGATAGATAGA-- A. 9 bands on the gel (or capillary electrophoresis) B. 6 bands on the gel (or capillary electrophoresis) C. 3 bands on the gel (or capillary electrophoresis) D. 2 bands on the gel (or capillary electrophoresis) E. 1 band on the gel (or...
Gel Electrophoresis of Amplified PCR Samples 9. What is Alu? 10. Why is Alu useful for...
Gel Electrophoresis of Amplified PCR Samples 9. What is Alu? 10. Why is Alu useful for studying human ancestry? 11. Why do the two possible PCR products from our lab differ in size by 300 base pairs? 12. Explain how gel electrophoresis separates DNA fragments 13. Fill in the table below. For each genotype, write how many DNA bands (fragments) you would expect to see in a gel, along with the size of each (n base pairs) Table 1. Predicted...
II. Amplify the gene of interest using the PCR, verify the PCR products on the gel...
II. Amplify the gene of interest using the PCR, verify the PCR products on the gel electrophoresis, analyze the gel. 4. By the next morning, the PCR amplification of all the samples is over, and you set up gel electrophoresis to check for the PCR products. Results: Lane 1 Alexandra - one band Lane 2 Olga - one band Lane 3 Tatiana - one band Lane 4 Maria - one band Lane 5 Anastasia - one band Lane 6 Alexey...
1. you have performed PCR and run your samples on a gel. When you look at...
1. you have performed PCR and run your samples on a gel. When you look at the gel, you see very bright bands of DNA- what is true about this experiment A) there is ethidium bromide in the gel B) the PCR machine was not working C) you "ran to red" (samples run towards the positive electrode) D) you "ran to black" and ran your samples off the gel E)all of the above are reasons for the bright bands 2....
The following family tree contains many members who had or have the disease "Emseebeeitis" (indicated by...
The following family tree contains many members who had or have the disease "Emseebeeitis" (indicated by the shaded circles and squares) (circles represent females and squares represent males). Those individuals who are healthy are indicated by unshaded figures. Those individuals who died have a slash mark. Those people who are still alive (1-16) were analyzed for a particular microsatellite "BH106". 1 2 3 DNA was isolated from each surviving member and microsatellite "BH106" was amplified by PCR and products were...
Two experiments were performed in order to confirm the Beta-glucuronidase gene from E. coli was present...
Two experiments were performed in order to confirm the
Beta-glucuronidase gene from E. coli was present in the pET28a
plasmid. (Lane1) PCR was performed to amplify the
Beta-glucuronidase gene and if the gene was present then a single
band would appear at ~7,300 bp. However, the band appeared to drag
down the gel but stopped ~7,300 bp. What would cause the PCR
product to smear down the gel and does this mean that the
amplification of the PCR product was...
The PCR procedure can be performed with fluorescently labeled primers and polyacrylamide gel electrophoresis (PAGE). What...
The PCR procedure can be performed with fluorescently labeled
primers and polyacrylamide gel electrophoresis (PAGE). What would
be the advantage of this procedure over agarose gel analysis?
PAGE allows for higher resolution than the agarose gel
procedure.
The PAGE procedure is faster than that using agarose gel
electrophoresis.
The labeled primers are less expensive because they are used at
a lower concentration in the reaction mix.
The PAGE procedure is less prone to contamination
Case Study 12-2 A 7-month-old child...
no experiment is being performed 9. What is the final PCR product, the stuff contained in...
no
experiment is being performed
9. What is the final PCR product, the stuff contained in your 12 tubes? 10. What is the purpose of gel electrophoresis? 11. The sequencer used in this lab has a thin capillary tube. What is the tube filled with? 12. How do DNA molecules move in relation to charge? Why?
You do a PCR reaction with primers to the D1S80 locus. You use your TAs DNA...
You do a PCR reaction with primers to the D1S80 locus. You use your TAs DNA as the template. Assume there are no primer-dimer formations or non-specific priming events. What banding patterns could you possibly see on the gel after you complete the PCR reaction? a) 1 band only b) 2 bands c) 3 bands d) a and b are possible e) all of the above are possible f) impossible to predict
You do a PCR reaction with primers to the D1S80 locus. You use your TAs DNA...
You do a PCR reaction with primers to the D1S80 locus. You use your TAs DNA as the template. Assume there are no primer-dimer formations or non-specific priming events. What banding patterns could you possibly see on the gel after you complete the PCR reaction? a) 1 band only (INCORRECT) b) 2 bands c) 3 bands d) a and b are possible e) all of the above are possible f) impossible to predict
What would you expect to see on a gel if PCR is performed for a microsatellite in a person with a genotype indicated by: (Allele 1: 3 TAGA repeats) (Allele 2: 6 TAGA repeats) ---TAGATAGATAGA------ ---TAGATAGATAGA TAGATAGATAGA-- A. 9 bands on the gel (or capillary electrophoresis) B. 6 bands on the gel (or capillary electrophoresis) C. 3 bands on the gel (or capillary electrophoresis) D. 2 bands on the gel (or capillary electrophoresis) E. 1 band on the gel (or...
Gel Electrophoresis of Amplified PCR Samples 9. What is Alu? 10. Why is Alu useful for studying human ancestry? 11. Why do the two possible PCR products from our lab differ in size by 300 base pairs? 12. Explain how gel electrophoresis separates DNA fragments 13. Fill in the table below. For each genotype, write how many DNA bands (fragments) you would expect to see in a gel, along with the size of each (n base pairs) Table 1. Predicted...
II. Amplify the gene of interest using the PCR, verify the PCR products on the gel electrophoresis, analyze the gel. 4. By the next morning, the PCR amplification of all the samples is over, and you set up gel electrophoresis to check for the PCR products. Results: Lane 1 Alexandra - one band Lane 2 Olga - one band Lane 3 Tatiana - one band Lane 4 Maria - one band Lane 5 Anastasia - one band Lane 6 Alexey...
The following family tree contains many members who had or have the disease "Emseebeeitis" (indicated by the shaded circles and squares) (circles represent females and squares represent males). Those individuals who are healthy are indicated by unshaded figures. Those individuals who died have a slash mark. Those people who are still alive (1-16) were analyzed for a particular microsatellite "BH106". 1 2 3 DNA was isolated from each surviving member and microsatellite "BH106" was amplified by PCR and products were...
Two experiments were performed in order to confirm the
Beta-glucuronidase gene from E. coli was present in the pET28a
plasmid. (Lane1) PCR was performed to amplify the
Beta-glucuronidase gene and if the gene was present then a single
band would appear at ~7,300 bp. However, the band appeared to drag
down the gel but stopped ~7,300 bp. What would cause the PCR
product to smear down the gel and does this mean that the
amplification of the PCR product was...
The PCR procedure can be performed with fluorescently labeled
primers and polyacrylamide gel electrophoresis (PAGE). What would
be the advantage of this procedure over agarose gel analysis?
PAGE allows for higher resolution than the agarose gel
procedure.
The PAGE procedure is faster than that using agarose gel
electrophoresis.
The labeled primers are less expensive because they are used at
a lower concentration in the reaction mix.
The PAGE procedure is less prone to contamination
Case Study 12-2 A 7-month-old child...
no
experiment is being performed
9. What is the final PCR product, the stuff contained in your 12 tubes? 10. What is the purpose of gel electrophoresis? 11. The sequencer used in this lab has a thin capillary tube. What is the tube filled with? 12. How do DNA molecules move in relation to charge? Why?
Most questions answered within 3 hours.
-
With which of the following anions will the lithium ion form an
insoluble salt?
a) sulfate,...
asked 1 minute ago -
You are still fascinated by the process of inkjet printing, as
described in the opening storyline...
asked 2 minutes ago -
What does the grounding mean especially at EMI point of
view?
asked 4 minutes ago -
Two dogs pull horizontally on ropes attached to a
post; the angle between the ropes is...
asked 5 minutes ago -
What would be the minimum sample size for an independent samples
t-test with a small effect...
asked 29 minutes ago -
In Java
The square. In one program, write 2 separate functions, and use
them.
Prompt the...
asked 35 minutes ago -
Hi guys! I need help for the Data Structure class i need to
provide implementation of...
asked 45 minutes ago -
Here's what we know:
-companies have lost loyalty from employees due to the quest to
cut...
asked 48 minutes ago -
One way to find prime numbers less than n is to treat them as
array indices....
asked 52 minutes ago -
Consecutive ideal polarizers have axes that are at a 60◦ angle
with respect to each other....
asked 1 hour ago -
Animals in cold climates often depend on two layers of
insulation: a layer of body fat...
asked 1 hour ago -
When bond rating companies rate a bond, which are qualities they
evaluate? Pick more than 1...
asked 1 hour ago