Homework Answers
9 . PCR final product from small RNA sequencing primer to multiplexing index read sequencing primer , it is nothing but a mixture of DNA of variable lengths.
10. Gel electrophoresis is employed to separate biological molecules by size . It is done by placing the same in gel with small pores and creating electric field across the gel. The molecules move faster / slower based on their respective sizes and electric charges.
11. The capillary tube is filled with buffer solution. ( It contains proteolytic enzymes that degrades the cell wall)
12. DNA fragments are ofcourse negatively charged that makes them move towards the positive electrode( once electric field is created across the gel) DNA fragments possess same amount of charge/ mass , so small fragments does move faster than large ones.
Add Answer to:
no
experiment is being performed
9. What is the final PCR product, the stuff contained in...
CALCULATIONS [2 points each] You are asked to perform a PCR experiment in the lab. However, in th...
CALCULATIONS [2 points each] You are asked to perform a PCR experiment in the lab. However, in this experiment you must add each ingredient separately (unlike the "master-mix" we used in our own experiment). In this experiment, each PCR reaction tube contained the following ingredients added with a micropipetor... 10 HL of 5x Buffer 6 μ1 of 25 mM MgCl2 solution μL of 10 mM dNTP mix 1.5 μL oligonucleotide primer mix 0.5 HL Taq DNA Polymerase stock solution 28...
En (2 points) You isolated your mitochondrial DNA in Part I. In step 6, you discard...
En (2 points) You isolated your mitochondrial DNA in Part I. In step 6, you discard the supernatant, but keep the pellet. In step 15, you discard the pellet, but keep the supernatant. Explain why the pattern is different between the two steps and the consequence of mixing up these two steps. Procedure Part 1: mt DNA Isolation from your cheek cells. Lysis solution is used to breakdown the cells in this step, you will isolate MEONA from cheek cells....
Gel Electrophoresis of Amplified PCR Samples 9. What is Alu? 10. Why is Alu useful for...
Gel Electrophoresis of Amplified PCR Samples 9. What is Alu? 10. Why is Alu useful for studying human ancestry? 11. Why do the two possible PCR products from our lab differ in size by 300 base pairs? 12. Explain how gel electrophoresis separates DNA fragments 13. Fill in the table below. For each genotype, write how many DNA bands (fragments) you would expect to see in a gel, along with the size of each (n base pairs) Table 1. Predicted...
The PCR procedure can be performed with fluorescently labeled primers and polyacrylamide gel electrophoresis (PAGE). What...
The PCR procedure can be performed with fluorescently labeled
primers and polyacrylamide gel electrophoresis (PAGE). What would
be the advantage of this procedure over agarose gel analysis?
PAGE allows for higher resolution than the agarose gel
procedure.
The PAGE procedure is faster than that using agarose gel
electrophoresis.
The labeled primers are less expensive because they are used at
a lower concentration in the reaction mix.
The PAGE procedure is less prone to contamination
Case Study 12-2 A 7-month-old child...
Your lab instructor has given you a protocol to perform a molecular cloning experiment. In a...
Your lab instructor has given you a protocol to perform a molecular cloning experiment. In a previous experiment, you used polymerase chain reaction (PCR) to amplify a sequence that you believe to regulate expression of a gene you are studying. You will now take this purified PCR product (double stranded DNA) and ligate it into a plasmid that contains a luciferase reporter gene. If your DNA sequence is a promoter sequence, then its presence will allow for expression of the...
I need the answers for questions 2 and 3. My DNA ladder is in lane 2...
I
need the answers for questions 2 and 3. My DNA ladder is in lane 2
marked by the yellow arrow. Thanks!
Here is the only other info I have. Thanks!
Part 2: Gel purification and on Gel Slice and PCR Product Preparin modified from TBSci.com instructions for gaan A. Dissolving the Gel Stie Following electrophores, eral DNA band from grand place glice microcentrifuge tube Ib. Use an analytical balance to weigh pelice Rec die 2. Add 500 balance to...
8. PCR is used to. A Diagnose genetic disease 8 Solve cnmes C Sudy gene unction...
8. PCR is used to. A Diagnose genetic disease 8 Solve cnmes C Sudy gene unction D. All of th C ONA as a template to form RINA D All of the above 7. PCR technique does not need A. Tag polymerase B Restriion encymes C Olgoucletide prmers C. A fragment of skin D. All of the above 9 PCR can be used in A Cloning B.Sequening C.Medical dagnosis&foric mine 0.PCR can make mullple copies ot A. DNA B RNA...
Experiment 1: Diffusion through Gelatin Data Tables Purpose (What question is this experiment designed to answer?):...
Experiment 1: Diffusion through Gelatin
Data Tables
Purpose (What question is this experiment
designed to answer?):
Hypothesis (Based on what you’ve learned in the
pre-lab materials, write and If/Then statement regarding the
outcome of this experiment.):
Table 1: Diffusion Data
Test Tube
Distance After 4 Hours (mm)
Distance After 24 Hours (mm)
A
B
C
Claim (What are the results of your
experiment?):
Evidence (What data did you collect that
supports your claim? Explain the meaning behind the data and...
solve for gel preparation and PCR mastermix calculations with steps for understanding TBE Buffer Calculations Determine...
solve for gel preparation and PCR mastermix calculations with
steps for understanding
TBE Buffer Calculations Determine the mass of the following reagents for a 10X stock 700mM of Tris Base (157g/mol) 887mM of Boric Acid (62g/mol) 25.7mM of EDTA (292g/mol) Dissolve in 750ml of DIH.O and bring to volume (IL) Calculate the dilution of your 10x stock for a 1X working stock. Remember you only need IL of working stock for a single experiment. Gel Preparation Calculations You need to...
What would happen if BsteII-HF had an optimal temperature of 30°C but we used the same...
What would happen if BsteII-HF
had an optimal temperature of 30°C but we used the same double
digestion protocol as we used in lab 4? (2pts)
Note: consider all the cloning steps for this
question
Double digestion of the the CAN1.Z ORNA and the PMD2 plasmid Bola I-HF SCHI CANL.Z! CANL.Z -SHI ©- MD2 CANLY → PMD2 CANLLY Bok I-HF You will cut both the plasmid PMD2 and the CAN1.Z ORNA using 2 enzymes (Bstell-HF and Sphl). These are two...
CALCULATIONS [2 points each] You are asked to perform a PCR experiment in the lab. However, in this experiment you must add each ingredient separately (unlike the "master-mix" we used in our own experiment). In this experiment, each PCR reaction tube contained the following ingredients added with a micropipetor... 10 HL of 5x Buffer 6 μ1 of 25 mM MgCl2 solution μL of 10 mM dNTP mix 1.5 μL oligonucleotide primer mix 0.5 HL Taq DNA Polymerase stock solution 28...
En (2 points) You isolated your mitochondrial DNA in Part I. In step 6, you discard the supernatant, but keep the pellet. In step 15, you discard the pellet, but keep the supernatant. Explain why the pattern is different between the two steps and the consequence of mixing up these two steps. Procedure Part 1: mt DNA Isolation from your cheek cells. Lysis solution is used to breakdown the cells in this step, you will isolate MEONA from cheek cells....
Gel Electrophoresis of Amplified PCR Samples 9. What is Alu? 10. Why is Alu useful for studying human ancestry? 11. Why do the two possible PCR products from our lab differ in size by 300 base pairs? 12. Explain how gel electrophoresis separates DNA fragments 13. Fill in the table below. For each genotype, write how many DNA bands (fragments) you would expect to see in a gel, along with the size of each (n base pairs) Table 1. Predicted...
The PCR procedure can be performed with fluorescently labeled
primers and polyacrylamide gel electrophoresis (PAGE). What would
be the advantage of this procedure over agarose gel analysis?
PAGE allows for higher resolution than the agarose gel
procedure.
The PAGE procedure is faster than that using agarose gel
electrophoresis.
The labeled primers are less expensive because they are used at
a lower concentration in the reaction mix.
The PAGE procedure is less prone to contamination
Case Study 12-2 A 7-month-old child...
I
need the answers for questions 2 and 3. My DNA ladder is in lane 2
marked by the yellow arrow. Thanks!
Here is the only other info I have. Thanks!
Part 2: Gel purification and on Gel Slice and PCR Product Preparin modified from TBSci.com instructions for gaan A. Dissolving the Gel Stie Following electrophores, eral DNA band from grand place glice microcentrifuge tube Ib. Use an analytical balance to weigh pelice Rec die 2. Add 500 balance to...
8. PCR is used to. A Diagnose genetic disease 8 Solve cnmes C Sudy gene unction D. All of th C ONA as a template to form RINA D All of the above 7. PCR technique does not need A. Tag polymerase B Restriion encymes C Olgoucletide prmers C. A fragment of skin D. All of the above 9 PCR can be used in A Cloning B.Sequening C.Medical dagnosis&foric mine 0.PCR can make mullple copies ot A. DNA B RNA...
Experiment 1: Diffusion through Gelatin
Data Tables
Purpose (What question is this experiment
designed to answer?):
Hypothesis (Based on what you’ve learned in the
pre-lab materials, write and If/Then statement regarding the
outcome of this experiment.):
Table 1: Diffusion Data
Test Tube
Distance After 4 Hours (mm)
Distance After 24 Hours (mm)
A
B
C
Claim (What are the results of your
experiment?):
Evidence (What data did you collect that
supports your claim? Explain the meaning behind the data and...
solve for gel preparation and PCR mastermix calculations with
steps for understanding
TBE Buffer Calculations Determine the mass of the following reagents for a 10X stock 700mM of Tris Base (157g/mol) 887mM of Boric Acid (62g/mol) 25.7mM of EDTA (292g/mol) Dissolve in 750ml of DIH.O and bring to volume (IL) Calculate the dilution of your 10x stock for a 1X working stock. Remember you only need IL of working stock for a single experiment. Gel Preparation Calculations You need to...
What would happen if BsteII-HF
had an optimal temperature of 30°C but we used the same double
digestion protocol as we used in lab 4? (2pts)
Note: consider all the cloning steps for this
question
Double digestion of the the CAN1.Z ORNA and the PMD2 plasmid Bola I-HF SCHI CANL.Z! CANL.Z -SHI ©- MD2 CANLY → PMD2 CANLLY Bok I-HF You will cut both the plasmid PMD2 and the CAN1.Z ORNA using 2 enzymes (Bstell-HF and Sphl). These are two...
Most questions answered within 3 hours.
-
A car travels at an average speed of 56 mph for 15 hours. What
was the...
asked 10 minutes ago -
Exercise on Mirror, Mirror on the Wall/lnitial Self
Evaluation
Write an Initial Self Evaluation paper that...
asked 1 hour ago -
Implementation of policy recommendations is often a
problem. What do you see as the major barriers...
asked 2 hours ago -
23- The consideration needed to support a contract
must:
a. Flow to the promise AND have...
asked 4 hours ago -
1. What is the main causal factor of Border line personality
disorder? Research has also found...
asked 6 hours ago -
The National Institute of Standards and Technology (NIST)
supplies "standard materials" whose physical properties are
supposed...
asked 6 hours ago -
Alpha Centauri A and B are Sun-like stars, and together they
form the binary star Alpha...
asked 6 hours ago -
What is the pH of a 0.200 M solution of sulfurous acid? Given:
Ka1 = 1.70×10–2,...
asked 6 hours ago -
Sociologists have conducted much interesting research on gender
stereotypes in American society. A curious aspect of...
asked 6 hours ago -
1. Based on your results, calculate how many kilograms of plant
material you would need to...
asked 6 hours ago -
Sample (taken every two minutes)
Angle of Rotation
0
102.9
2
104.2
4
106.2
6
104.8*...
asked 6 hours ago -
Adrian's utilities of two consumption bundles are 50 and 100,
respectively. This implies that A. Adrian...
asked 6 hours ago