Question

What is the difference between two forms of HIV? What is the principle of DPP HIV 1/2 ASSAY test?

Answer 1

o Weakness of immune system cause of HIV viruses. There are two main types of HIV virtuves tie HIV-1 HIV-2 - It develop very quickly - It tends to develope in human body of her more & locuely than & It shows the symptoms - HIV-2 may have a HIV-1 very rapidly manner. long period without -> HIV-1 is the most comm- 4 HIV-2 any Symptoms than HIV-1. mainly present on in all over world in West Africa, including some other country - India, Europe also. Tranmission rate is higher -89 ransmission rate is 20tr toneer than HIV-1. It is responsive to . It is less responsive centain dougs like set to these drugs. enturintide consolnog bussoign pribrid @ Princeples of DPP HIV 1/2 Assay :- ) - DPP C Dual path platform) is a immunochro- matographie test for the detection of above Emmunodeficiency virus type 1 and 2 (HIV112 * Biological Principle of test : - 2 These technology consist 2 Path as Sample Path hout bi Reagent Path which intersect in the detection of antibody 1 Crest and control) zones in the readout window of test cessette.

- To Enitiate the test, a speciman in collected and applied to SAMPLE + BUFFER wel of the test cessette - The sample flours along the sample path membrane and delivered to test zone of reagent Strip, where specific HIV antigen and control (protein A) are immobitired, in the test one. 3 If HIV antibodies present in the sample h the it binds to immobilired test antigens, While nonspecific rq G binds to protein A Contror sinal - five minute after adding the sample, vi buffer is added to BUFFER well. - The butter hydrates the dried antibody binding coloured conjugate, which mignă - te to test zone. en If the sample contain either of antiba Odies, the rest are producing a pink sir or purple line. If absence of these antibodies then colour formation not! occurs. - Mhe liquid continulously migrate through membrane producing colour band in CONTROL area contain Protein A. tione - This control serves to demonstrates the speriman and reagents have properly applied and mignałe through device