Question

EFFECT OF pH:

1. Bubble height indicates the degree of enzyme activity. Based on the results of your experiment, which tube had the highest bubble column height?

What can you conclude about this pH? 2. Explain the results of the other two tubes. What is your conclusion concerning the effect of pH on enzyme activity?

Results: Table 5.1 - Catalase Activity Tube # Contents Bubble Column Height (mm) catalase/H202 dH20/H202 catalase/sucrose 27mm Omm | Omm E Table 5.2 - Effect of Temperature Tube # Temp. (°C) Bubble Column Height (mm) 0 16mm 25 14mm 37 4mm 100 1 mm 4 E Table 5.3- Effect of Enzyme Concentration & Time Bubble Column Height (mm) after 20 Bubble Column Height (mm) after 2 min. sec. Tube Amt. of Enz. O ml 1 ml 3 3 ml 10 15mm 12mm 10mm 24mm E Table 5.4 - Effect of pH Tube # pH | 3 6.6 11 Bubble Column Height (mm) 4mm 0 o

Answer 1

Tube # Contents Bubble Column Height (mm) 11 catalase/H202 dH20/H202 catalase/sucrose | 2 3 This is the control table, were the positive control of catalase activity is in tube 1 and negative controls in tube 2 and 3. There is not much to say here. The normal bubble column height in the catalase activity under normal conditions is 27 mm 27mm Omm Omm sec. Here, we observe the effect of temperature. At o C, the catalase Tube # Temp. (°C) Bubble Column Height (mm) activity looks affected in comparison of the tube 1 in the Table 0 16mm 5.1. This means that the enzyme has an optimal temperature, 25 14mm below or above that temperature, the enzyme activity will be 37 4mm affected. Under high temperatures the catalase activity decrease 100 1mm considerably. At high temperatures, the enzyme can be denatured, so this may be the reason for such low activity In this table, we can observe that the enzyme Bubble Column Height (mm) after 20 Bubble Column Height (mm) after 2 min. concentration influence the enzymatic activity, being the highest concentration (tube 3) the highest enzymatic activity Finally, we can observe that under acidic pH, the enzyme can work | Tube # pH | Bubble Column Height (mm) || slowly. However, under basic pH, the enzymatic activity is null. This 13 4mm is because each enzyme has an optimal pH. If we use a pH below or 6.6 10 above the optimal pH, the enzymatic activity will be affected. 11 0 However, it is interesting that we did not observe any enzymatic activity at pH 6.6 and 11. Indeed, catalase can work at from pH 7 to 11 normally. Tube Amt. of Enz. Omlo | 1 ml 3 ml 15mm 12mm 3 10mm | 24mm 2 3