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Introduction: Prediction: Table 5-5 Effect of pH on Enzyme Activity /2 me Tube 5A Tube 5e Tube 5c Tube 5o Tube 5e Tube 5: Tube 5 0 min 10 min. 3O Figure 5-6 Eff ect of pH on catechol oxidase activity. Use your data to produce a graph of the effects of pH on catechol oxidase activity Discussion 1. What is the pH range of catechol oxidase? 2. What is the optimum pH for catechol oxidase? 3. What can you conclude about the temperature range vs. the pH range of this enzyme?
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Answer #1

From the given data,

i. pH range of Catechol oxidase = 6 - 8

Below pH= 6 and above pH = 8, the enzyme shows reduced function.

ii. Optimum pH = 7

At pH = 7, the enzyme shows maximum activity.

iii. The optimum temperature for the enzyme = 32C to 37C.

Enzymes have limited optimum pH range compared to optimum temperature range.

This is because of the fact that the charge properties of amino acids are highly sensitive to changes in pH than changes in temperature.

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