What is the role of the Lambda HIND III digest in the gel experiment.
What is the role of the Lambda HIND III digest in the gel experiment.
Use the following depiction of a gel of Hind III fragments to answer the questions: 1 Kb Ladder Possible Hind III Fragments: 1 Kb Ladder Sizes 10,000 8,000 564 bp 8,888 2.0 kb 2.3 kb 11 4.36 kb 4,000 3,000 2,500 2,000 1,500 6.5 kb 9.4 kb 23 kb 1,000 750 500 250 1. Based on the gel results, list the sizes of the chromosome fragments cloned: 2. Based on the gel results, list the sizes of the chromosome fragments...
9. On Worksheet 16.IIIB is a restriction map of bacteriophage lambda. You digest some lambda DNA with the enzymes BamHI and HindIII separately and then load the fragments into an agarose gel and perform electrophoresis. Next, you perform a Southern analysis using the 4,878-bp EcoRI lambda fragment as a probe. a. Draw a picture of the electrophoresis gel, using the outline of the stained electrophoresis gel in Worksheet 16.IIIB (the two smallest HindIII fragments will run off the gel.) b....
3. The given figure represents the agarose gel electrophoresis results from a restriction digest experiment. Lane 1 is a DNA ladder (values are in kb) and Lane 2 is the DNA sample cut by a restriction enzyme. What is the size of the band C in lane 2? - 1111111 About 1.8 kb Cannot tell based on the information provided 500 bp About 2 kb © Less than 1.5 bp -
can someone explain throughly on how to find a-c??? thanks!!! The following question will provide practice in interpreting and analyzing gel results. 5. You obtained the DNA electrophoresis gel below. Three samples of lambda phage DNA were digested with 3 different restriction enzymes and the digested DNA was applied to the gel in lane 4 and the bands were visualized. The Hind Ill digest was used as a molecular weight standard marker and produced 6 DNA fragments of known size:...
on the me but not with the larger fragments on o t and more and record med in the h 3. Determine the distracted for each bund data bere 4. Determine the distance traveled for each and generated in the double digest and record data bere tion Endonuclease Digestion and Gel Electrophoresis of DNA 185 VI. RESTRICTION MAPPING The different DNA fragments generated by different map DNA. For example, specific DNA on 2000 nucleotides in las Rl or Hind and...
Hi I have a problem with number 5, it involves gel analysis results. There are 2 parts, a,b,c. For A Im sure you need to make a graph with distance in (cm) on the vertical axis and log10 bp on the horitzontal. I need help figuring out where to start and what to do. Please help! The following question will provide practice in interpreting and analyzing gel results You obtained the DNA electrophoresis gel below. Three samples of lambda phage...
A DNA molecule is cleaved with Hind III, Pst II, or the two enzymes together. The accompanying diagram shows the resulting electrophoresis gel, with band sizes indicated. Is the DNA molecule in question linear or circular? Linear circular Using the information in question 6. determine where the Pst 1 restriction site is located. Pstl cleave within the 7kb HindM fragment or within the 3kb HindM fragment'; 7kb fragment 3kb fragment neither fragment both fragments
You digest a 10Kb Linear ECoRI dna fragment with TWO restriction enzymes and obtain the following data Hind iii..... 3kb, 7kb BamHi ... 2 kb and 8kb HInd iii + bamHI ..... 1kb, 2kb, 7kb Draw a restriction map of this DNA fragment, labeling the sites for EcoRI, bahHI, and HIndiii
1. How many fragments were produced? 2. What is the size of the fragments? Custom Digest NEW ENGLAND Print Close BioLabs NEBcutter unnamed sequence - digested with: Alul mment Gel Type: DNA Type: Unmethylated Marker: 1% agarose Lambda - Hindll Digest L=102 mm OK Length (bp) Ends Coordinates narker 1| (LeftEnd)-Alul 1-167 2Alul-(RightEnd) 217-308 3 Alul-Alul 167 92 49 10000 168-216 5000 1000 500 100
Examine the following gel. Each lane contains a digest; the same plasmid is in each lane, but it has been digested with a different combination of enzymes. Based on the pattern, identify the plasmid represented on the gel. (B=BamHI, E=EcoRI, H=HindII) E HB/E B/ HE/H |||||||||| Plasmid a O Plasmid b O Plasmid c None