a. C is DNA polymerase, which different in both eukaryotes and prokaryotes.
DNA polymerase III is involved in prokaryotes
DNA polymerase
is involved in eukaryotes for replication which extend the primer
DNA ploymerase
is involved in priming.
b. Replication protein A (RPA) is single stranded binding protein. during replication,RPA prevents single-stranded DNA (ssDNA) from winding back on itself and prevent from forming secondary structure. if RPA do not perform any function, DNA polymerase would not able to replicate as single strands as template would not present for replication.
SSB in prokaryotes
Replication protein A (RPA) in eukaryotes
a)b) topoisomerase IT As the replication fork continues to open, what will happen to the protein...
t sequence of enzymes necessary for DNA replication are he Meli Primase, Topoisomerase. b) Helicase To c) Helicase, Ton merase, Primase, DNA D merase II, and DNA Ligase erase, DNA Polymerasei t , and DNA Ligase d) Helicase, Topoisomerase, Primase, DNA Polymerase I, and DNA Ligase dDNA Ligase 58. DNA tesized in the 31 5' direction b) False 59. Eukaryotes have a single origin or replication a) True b) False Okaz 6U- KI Fragments are synthesized in the 3'-5' direction...
1. If DNA polymerase III was going to attach a new nucleotide onto the DNA molecule shown to the right, where would it attach it? 2. Why are the ends labeled A and C different? a. The diagram is drawn incorrectly, they should be the same One is o pure onsa pyrimidine. c. One is a purine, one is a pyrimidine d. Double-stranded DNA is antiparallel. e. This is after DNA replication -before DNA replication, they look the same. 3....
In the following diagram, A and B are two Okazaki fragments
generated during DNA replication. Open boxes represent the primers
and dotted lines represent the newly synthesized DNA strands.
a. To which direction (left or right) is the replication fork is
moving? (1 mark)
b. Which Okazaki fragment is made first in the diagram? Explain.
(2 marks)
c. (i) Which enzyme in E. coli synthesizes the primers on
Okazaki fragments? (1 mark)
(ii) What is the difference of this enzyme...
In the following diagram, A and B are two Okazaki fragments generated during DNA replication. Open boxes represent the primers and dotted lines represent the newly synthesized DNA strands. B ---- Template DNA a. To which direction (left or right) is the replication fork is moving? (1 mark) b. Which Okazaki fragment is made first in the diagram? Explain. (2 marks) c. (i) Which enzyme in E. coli synthesizes the primers on Okazaki fragments ? (1 mark) (ii) What is...
b) What property p it to be functional"? Explain your sr Detity (or properties) of R be replcafec Snl sh RNA c) Which of t a "functional RNAz Chthe folowing CRN mNAR (2pts) Either: i) Pick any two types what ea d) at are functional RNAs, and briefly state each one does (make clear which two you have chosen), or i) Name the three types y of RNAs that participate in translation, and state what role each plays in this...
TranslationOverview:The purpose of this activity is to help the students to understand how replication, transcription, and translation are connected. Students will use a sequence from a bacterial gene that confers resistance to antibiotics (carbapenems). They will be asked to apply the knowledge obtained in the class lecture to (1) find the promoter in the sequence, (2) determine the amino acid sequence of a fragment of the polypeptide, (3) "reverse translate" a fragment of the polypeptide, and (4) identify mutations in...
Genetics Worksheet Week 3: Gene Regulation and Epigenetics 1. Duchenne muscular dystrophy is caused by a mutation in a gene that is 2.5 million nucleotides in length and encodes a protein called dystrophin. The dystrophin protein itself is 3684 amino acids in length. Calculate below the approximate size of the mRNA that encodes dystrophin. Approximately what percentage of the gene that encodes dystrophin is intron sequence? The human genome encodes a much greater variety and number of proteins than the...
HIV is a retrovirus (a virus that uses reverse transcriptase). a. What is reverse transcriptase? b. How is a retrovirus different from other viruses? c. How does a retrovirus infect a cell and reproduce itself? 2. Review of the immune system. a. What is a T cell? b. What varieties of T cell exist? How are they functionally different? c. What are their roles in the human body? d. How is each T cell variety differentiated from the others (molecularly)?...
PLEASE COMPUTER TYPED* OR VERY CLEAR HANDWRITING with
details
1. Radioactive (14C) uridine (lots of it) is added to a growing culture of bacteria for 10 seconds and then the drug rifamycin is added (rifamycin blocks the initiation of transcription, but not continued elongation of RNA polymerases that have already begun transcribing). At two-minute intervals, 1 ml samples are removed and placed on ice. The bacteria are lysed (broken open) and an enzyme is added that will chew up all...
25. Mendel's factors undergo segregation and independent assortment. How is this illustrated in the chromosomes during Meiosis I? 26. Explain how these inheritance patterns are considered non-Mendelian. Incomplete Dominance . Multiple Alleles • Codominance X-linked Linkage . Pedigrees - Genetic Disorders 27. What is non-disjunction and how does it affect the chromosome distribution during meiosis? 28. What is a karyotype and what does it allow you to do? 29. Fill in the circles and squares to illustrate the following inheritance...