Question

topoisomerase IT

a)As the replication fork continues to open, what will happen to the protein labeled “C”? Is this the same in prokaryotes and eb)Briefly explain what would happen if the protein RPA did not come in to perform its function in regards to primers. How will

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Answer #1

a. C is DNA polymerase, which different in both eukaryotes and prokaryotes.

DNA polymerase III is involved in prokaryotes

DNA polymerase \delta is involved in eukaryotes for replication which extend the primer DNA ploymerase \alpha is involved in priming.

b. Replication protein A (RPA) is single stranded binding protein. during replication,RPA prevents single-stranded DNA (ssDNA) from winding back on itself and prevent from forming secondary structure. if RPA do not perform any function, DNA polymerase would not able to replicate as single strands as template would not present for replication.

SSB in prokaryotes

Replication protein A (RPA) in eukaryotes

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