Nt= N0e^rt
Nt= number of cells after time t,
t= time
r= growth rate
here
N0= 500
t= 2 hours= 260=120
minutes
r= 0.049 per minute
Nt= 500
e^(120
0.049)
=178904.6 cells
number of cells after 2 hours= 178904.6 cells
2. A culture medium was inoculated with 500 cells and incubated for 2 hours where they...
3. A culture medium was inoculated with 1200 cells and incubated for 4 hours. At the end of incubation there were 106,000 cells. Calculate the generation time and growth rate of the culture. Show your work to get full credit. (5pts)
3. A culture medium was inoculated with 1200 cells and incubated for 4 hours. At the end of incubation there were 106,000 cells. Calculate the generation time and growth rate of the culture. Show your work to get full credit. (5pts)
2. Tonicity. (a) How do you prepare a % NaCl solution? Tell whether the medium is hypertonic, isotonic or hypotonic to the cell: 3. Generation Time. A microbiologist inoculated a sterile culture medium with bacteria. The generation time for the bacterium is How many cells form the generation? How many days would it take to produce generations of bacteria?
I need help with these questions for microbiology class.
inoculated a culture tube with 2 cells of E.coli. IfE. coli has a generation time of 60 minutes under the conditions at which the culture is stored, how many cells would be in the culture tube when she comes back 6 hours later? Hint: You do not need a calculator for this. If you are thinking you de. you are doing it incorrectlv. It is simple arithmetic 1 What kind of...
Example An otherwise typical bacterial cell increases from one cell to 256 cells in 10 hours. What is the generation time of this organism? Example: Escherichia coli has a doubling time of 20 minutes. If there are 5 cells at the beginning of the experiment, how many will there be in 3 hours? Example You perform a serial dilution and determine that the original number of cells in your sample was 12,000. How many bacteria will be present in 12...
The bacteria you are using grow with a generation time of 18 minutes in the medium that you are using. You inoculate the flask of medium with 8.00×104 bacteria. The flask is incubated for 180 minutes. Assume there is no lag phase and the culture does not reach stationary phase during the period of incubation. Within 5%, what is final number of bacteria in the flask? (Do not use scientific notation to enter your answer. Enter 4.2 x 107 as...
MICROBIO HELP: You want to grow up some bacteria to bring to class to show your friends, and you want to have enough to give everyone in the class 100 bacteria. If you are starting with only 5, how many generation times should you allow so you will have enough? (assume there are 130people in the class). If you knew it took 1 hour and 39 minutes to grow all your bacteria, what is the generation time? Helpful information provided:...
please answer both questions and show necessary
work
4. If Bacterium X has a generation time of 60 minutes and 10 Bacteria X cells are inoculated into broth, how many hours will it be before there are 10° Bacteria Y colls in the broth culture? You MUST SHOW YOUR WORK to get credit. (2 % pts. total) 5. Using the graph below, draw the expected growth curve for a bacterial microorganism that spends 60 minutes in the lag phase, undergoes...
A patient order specifies: "Nitroglycerin 100 mg in 500 mL D5W. Infuse over 6 hours." If the administration set delivers 60 drops/mL, how many milligrams of drug is this patient receiving per hour? If 500-mL of an intravenous solution contains 0.3 g of a drug, at what flow rate, in milliliters per minute, should the solution be administered to provide 0.5 mg/min of drug? 500-mL of an intravenous solution contain 0.6% of drug in sodium chloride injection. At what flow...
Doctor's order: 500 mL of 5% D 0.45 normal saline solution to infuse over 2 hours How many gtts per minute should be given if the tubing delivers 10 gtt/mL?!