Describe how Coomassie Blue dye interacts with protein and how the resulting complex influences the wavelength of light absorbed.
The Coomassie Brilliant Blue dye which is commonly used in SDS-PAGE was first described by the German scientist Volker Neuhoff. The dye gets its name from the African city Kumasi, formerly Coomassie, a city in central Ghana. Currently, there are two kinds of the Coomassie dyes: R-250 and G-250. Coomassie R-250 is the more commonly used and sensitive of the two. Though less sensitive, Coomassie G-250 has particular properties that enable it to be used to create a rapid and convenient staining procedure. In the staining reaction, the Coomassie dye binds to proteins through ionic interactions between sulfonic acid groups and positive protein amine groups through Van der Waals attractions. DNR developed unique Bio-Imaging systems that can easily detect the Coomassie Brilliant Blue dye in a quick, accurate and reproducible manner. Our superior GelCapture software and camera allow the user to detect both the highest and lowest band intensity.
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Describe how Coomassie Blue dye interacts with protein and how the resulting complex influences the wavelength...
Describe how Coomassie Blue dye interacts with protein and how the resulting complex influences the wavelength of light absorbed.
An octahedral metal complex absorbs light with wavelength 580 nm. a. What is the crystal field splitting A, in kJ/mol for the complex? (Hint: Energy is based on wavelength of light absorbed. See data sheet.) (3 marks) b. What color does the complex appear to the eye? (2 marks) 750 nm, 400 nm Red Violet 630 nm 430 nm Blue Orange 590 nm 480 nm Yellow Green 560 nm
Please help! Ligand X forms a complex with both cobalt and copper, cach of which has a maximum absorbance at 510 nm and 645 nm, respectively. A 0.217 g sample containing cobalt and copper was dissolved and diluted to a volume of 100.0 mL. A solution containing ligand X was added to a 50.0 mL aliquot of the sample solution and diluted to a final volume of 100.0 mL. The measured absorbance of the unknown solution was 0.493 at 510...
Describe the structure of a ribosome and its three sites. Explain how it interacts with an mRNA and tRNAs to build a protein.
When an activator protein interacts with a mediator protein, how does it affect the function of RNA polymerase?
Dye preparation mechanics How can I get this dye Pacific Blue ОН НО
How much energy does a photon of blue light with a wavelength of 465 nm have? How much energy does a photon of red light with a wavelength of 663 nm have? Photons of which color of light (red or blue) have greater energy? red or blue
The wavelength of light (in nanometers) that is absorbed by a complex that has a crystal-field splitting energy of 3.30×102 kJ/mol is _____. (h = 6.626 × 10−34 J·s, c = 3.00 × 108 m/s)
How many bonds? Indigo is the dye used in coloring blue jeans. The term navy blue is derived from the use of indigo to dye British naval uniforms in the eighteenth century. The structure of the indigo molecule is "С O How many ơ bonds exist in the molecule? bonds How many r bonds exist in the molecule? bonds Submit 0 What hybrid orbitals are used by the carbon atoms in the indigo molecule? orbitals Previous Next
[TI(H20)is an octahedral complex. (9 pts) 3. Name this complex. b. How many d electrons does it have? Sketch the d-orbital diagram for the complex. Label the energy levels (t and e and a. c. each of the d-orbitals. d. A is 3.99 x 10-19 ] (per photon). What is the value of A in kl/mol? e. Calculate the wavelength of light (nm) needed to excite an electron from the tag to the e, orbital. f. What color of the...