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The replication carried out by DNA polymerases is highly accurate. This low error rate is reached by the proofreading activity of DNA polymerases which removes a mismatched nucleotide that is wrongly incorporated by the enzyme. In addition, post replication DNA repair enzymes removes the mismatches (and incorporates the correct one) that are incorporated during replication.
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QUESTION 9 The error rate of DNA replication is reduced both by proofreading and by DNA...
Error rates in DNA replication, proofreading and mismatch repair systems in E. coli are about 10-10...
Error rates in DNA replication, proofreading and mismatch repair systems in E. coli are about 10-10 per base pair per round of replication. Based on this, how many mutations per base pair would you expect to see in a population of 1010 E. coli cells (e.g. of a TSB culture of E. coli)
21. In DNA replication, new nucleotides are added a. To the 5' end of each nascent...
21. In DNA replication, new nucleotides are added a. To the 5' end of each nascent strand b. To the 3'end of each nascent strand To both ends of each nascent strand d. To the 5' end of the continuous strand and the 3' end of the discontinuous strand e. To the end of the continuous strand and the end of discontinuous strand fragments 22. DNA unwinding is done by a. Ligase b. Helicase c. Topoisomerase dHexonuclease 23. Proofreading of...
Part A Which enzymatic activity is primarily responsible for the proofreading step of DNA replication? hydrolytic...
Part A Which enzymatic activity is primarily responsible for the proofreading step of DNA replication? hydrolytic editing tRNA synthetase activity O RNAseH activity O 3 to 5' exonuclease activity 5' to 3' exonuclease activity Submit Request Answer
A) Which property of DNA replication enables accurate mismatch repair to prevent mutation? a) Adenine methylase...
A) Which property of DNA replication enables accurate mismatch repair to prevent mutation? a) Adenine methylase lags behind the DNA polymerase b) the proofreading ability of DNA polymerase c) the error-free nature of DNA pol. III d) the sister chromatid can be used as a template B) which DNA repair mechanism uses the sister chromatid as a template for repair? a) nucleotide excision repair b) double-stranded break repair c) postreplication repair d) base excision repair C) indicate which substitutions require...
RNA polymerases lack proofreading ability. Define proofreading ability and describe its importance in replication of DNA...
RNA polymerases lack proofreading ability. Define proofreading ability and describe its importance in replication of DNA genomes. a. What advantage might its absence give RNA viruses like SARS-CoV-2? Following protein synthesis, RNA genome and proteins assemble to form virus particles that then bud into the endoplasmic reticulum (ER) lumen and then on to the Golgi apparatus. Next, they are transported via Golgi vesicles to the cell membrane, where they are released via exocytosis. Define ER, Golgi apparatus, and exocytosis,...
QUESTION 2 Which of the following statement is true for both DNA replication and transcription? A....
QUESTION 2 Which of the following statement is true for both DNA replication and transcription? A. Both polymerases catalyze the formation of phosphodiester bonds between nucleotides. B. Primers are required to provide 3'-OH group for both polymerases to initiate the synthesis of DNA or RNA. C. DNA and RNA synthesis both proceed in a 5' to 3' direction or, in other words, nucleotides are added to the 3'- OH groups of the growing chains. D. Two of the above. O...
Question 15 1 pts To study DNA replication, researchers often use bacteria that harbor a temperature...
Question 15 1 pts To study DNA replication, researchers often use bacteria that harbor a temperature sensitive mutation in one of the genes related to replication. This means that when cells are grown at lower temperatures (30°C) they are fine, but when shifted to 42°C the effect of the mutation becomes evident and that protein is no longer functional. Depending on the gene mutated, researchers observe that for some replication stops almost immediately, but for others more slowly. In this...
Semi-conservative DNA replication can be used by bacterial chromosome and plasmid both. a) True b) False
Semi-conservative DNA replication can be used by bacterial chromosome and plasmid both. a) True b) False
Question 14 4 pts Which one of the following enzymes WILL NOT participate in DNA replication?...
Question 14 4 pts Which one of the following enzymes WILL NOT participate in DNA replication? O RNA ploymerase O DNA polymerase Ligase O Helicase Question 15 4 pts In DNA replication - if one DNA strand has nucleotide sequence as 5'ATGCITCCT3, The other strand of the DNA will have nucleotide sequence as and it will be synthesized in direction. OUACGAAGGA; 3' to 5' direction OTACGAAGGA; 3' to 5' direction OTACGAAGGA; 5' to 3' direction OTUCGUUGGU; 5' to 3' direction
Both the leading strand and lagging strand in replication synthesize a new strand of DNA but...
Both the leading strand and lagging strand in replication synthesize a new strand of DNA but explain how each strand is made. Make sure to include important enzymes like ligase, helicase, topoisomerase, DNA polymerase III/I, and primase.
21. In DNA replication, new nucleotides are added a. To the 5' end of each nascent strand b. To the 3'end of each nascent strand To both ends of each nascent strand d. To the 5' end of the continuous strand and the 3' end of the discontinuous strand e. To the end of the continuous strand and the end of discontinuous strand fragments 22. DNA unwinding is done by a. Ligase b. Helicase c. Topoisomerase dHexonuclease 23. Proofreading of...
Part A Which enzymatic activity is primarily responsible for the proofreading step of DNA replication? hydrolytic editing tRNA synthetase activity O RNAseH activity O 3 to 5' exonuclease activity 5' to 3' exonuclease activity Submit Request Answer
QUESTION 2 Which of the following statement is true for both DNA replication and transcription? A. Both polymerases catalyze the formation of phosphodiester bonds between nucleotides. B. Primers are required to provide 3'-OH group for both polymerases to initiate the synthesis of DNA or RNA. C. DNA and RNA synthesis both proceed in a 5' to 3' direction or, in other words, nucleotides are added to the 3'- OH groups of the growing chains. D. Two of the above. O...
Question 15 1 pts To study DNA replication, researchers often use bacteria that harbor a temperature sensitive mutation in one of the genes related to replication. This means that when cells are grown at lower temperatures (30°C) they are fine, but when shifted to 42°C the effect of the mutation becomes evident and that protein is no longer functional. Depending on the gene mutated, researchers observe that for some replication stops almost immediately, but for others more slowly. In this...
Question 14 4 pts Which one of the following enzymes WILL NOT participate in DNA replication? O RNA ploymerase O DNA polymerase Ligase O Helicase Question 15 4 pts In DNA replication - if one DNA strand has nucleotide sequence as 5'ATGCITCCT3, The other strand of the DNA will have nucleotide sequence as and it will be synthesized in direction. OUACGAAGGA; 3' to 5' direction OTACGAAGGA; 3' to 5' direction OTACGAAGGA; 5' to 3' direction OTUCGUUGGU; 5' to 3' direction
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