Semi-conservative DNA replication can be used by bacterial chromosome and plasmid both.
a) True
b) False
Answer: True.
Explanation: Semi- conservative DNA replication is a model. DNA is a double strand structure, during replication each structure opens up and acts as a template for the synthesis of new complementary strand. This will result in two DNA molecules, and each DNA molecule having one old strand that is parent strand and one new strand. This is what semi- conservative DNA replication model is.
As the structure of DNA is same in all organisms bacterial chromosomes and plasmids too have semi-conservative model of DNA replication. However there may be minor modifications in each organisms according to their needs and survival.
Semi-conservative DNA replication can be used by bacterial chromosome and plasmid both. a) True b) False
Which of the following is NOT true about bacterial conjugation An F factor drives replication and transfer of a conjugative plasmid The genome of the bacterial chromosome in the donor cells is altered through ectopic recombination Transfer is mediated by a tube-like pilus formed between the adjacent cells Mating cells often break apart before transfer of the entire bacterial chromosome. The transferred DNA can be incorporated in the recipient cell bacterial chromosome via homologous recombination.
2. After each sentence below, note whether the sentence is true or false. If false, cross out one incorrect word in the sentence and write the correct one above it. a. DNA polymerase requires a short primer made of DNA with a 3' OH group to begin chromosome replication. b. Semi-conservative DNA replication means that a newly synthesized daughter chromosome will be fully- methylated c. The lagging strand of newly synthesized DNA will have many more primers compared to the...
Draw and describe the results of the Meselson-Stahl experiments showing that DNA replication followed the Semi-conservative model. Draw and describe the results if DNA replication turned out to follow the Conservative model.
1. Describe one experiment that can test the hypothesis that DNA replication is semi conservative. Describe the results of this experiment if replication was conservative. And if it was distributive? 2. What type of chemical bond contributes to the specificity of base paring in the DNA? Are there any other chemical or physical factors in the structure o f the DNA molecule contributing to the thermodynamic stability of the DNA? 3. List the m ajor differences between DNA and RNA....
Question 7 (1 point) Saved DNA replication occurs through the semi-conservative replication mechanism; what was observed in the Meselson and Stahl experiment, in which DNA purified from E. coli growing on 14N-medium was centrifuged in a density gradient tube, by the third generation of DNA replication? One band representing "light" DNA OTwo bands: a light density band and a faint hybrid density band Three bands: one each of light density, hybrid density, and high density bands One band representing "heavy"...
Draw a diagram showing a portion of a chromosome undergoing replication. Label the leading and lagging strands, and replication fork. What are the roles of DNA polymerase and RNA polymerase? How does this process represent semi-conservative replication?
The bacterial replication machinery has been isolated and examined in vitro. The best way to learn about the replication apparatus is to isolate it and them examine how each part works by itself and in concert with the other parts. Kornberg and coworkers worked very hard purifying all enzymes required for replication Initiation to try and study the process in detail. They then took the bacterial chromosome and digested it and plasmid DNA with restriction endonucleases (see below). They then...
4a. Define semi-conservative DNA replication. 4b. Explain why Recombinational Repair of thymidine dimers absolutely requires active DNA replication. 5a. Explain how the Ames Test works to identify whether a novel chemical (compared to water) is a DNA mutagen. b. Below, draw the plate results that would allow you to conclude that this novel chemical was in fact a mutagen compared to a plate with water as a negative control.
4a. Define semi-conservative DNA replication. 4b. Explain why Recombinational Repair of thymidine dimers absolutely requires active DNA replication. 5a. Explain how the Ames Test works to identify whether a novel chemical (compared to water) is a DNA mutagen. b. Below, draw the plate results that would allow you to conclude that this novel chemical was in fact a mutagen compared to a plate with water as a negative control.
Which of the following statements about eukaryotic DNA replication is false? A) DNA polymerase can add new nucleotides to either free 3'- or 5'-end B) RNA primers must be removed and rejected by the deoxynucleotides C) RNA primers must be laid down during DNA replication for synthesis of new DNA that starts at the 3'-OH of the primer ends D) Elongation of the DNA strand during replication requires primers with free 3'OH end E) The very end of the chromosome...