Homework Answers
When cells are grown for a number of generations in a medium containing radioactive thymine , their DNA contains radioactive thymine nucleotide in thier complete DNA .(Ignoring initial two cells which have 50% old DNA)
When these radioactive thymine containing cells are grown in a non radioactive thymine medium and they replicate once , then only 50% of their DNA would have been radioactive or we can say that only 50% part of these cells would have been radioactive(As the semiconservative nature of DNA).
When DNA is replicated , it contains one old strand and one newly synthesized strand.If the initially the complete DNA was radioactive , then in non radioactive medium , one helix will be newly synthesized and one will remain same , the 50% DNA will now been radioacitve only.
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Question 36 1 pts 36. You have a population of mammalian cells growing in media containing enough growth factors. Which of the following graphs corresponds to these cells when their DNA content is analyzed 24 hours after they are induced to express high levels of a mutant form of Rb protein that cannot be phosphorylated? (Note: this cell line has its cell cycle around 22 hours) Control cells #1 #2 #3 #4 #5 #6 Cell number JAN ul. 2 2...
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Please need help answering question A the pages of background information are posted thanks Read page...
Please need help answering question A the pages of background
information are posted thanks
Read page 196-197 and figure 6.20. regarding Meselson and
Stahl’s experiment regarding DNA replication. And Answer the
following question
If you are using this radioactive technique in mouse cells,
what would happen in each phase of G1, S, G2, mitosis and meiosis
assuming that you are grown cells in 15N medium for many
generations and cells in G1are then switched to 14N medium?
G1
S
G2...
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Please explain each part !! Thank you !!
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please answer all 5! thank you! Question 1 1 pts Which of these individuals would be...
please answer all 5! thank you!
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Please summarise the following content:(no word limit) EXPERIMENTAL PROCEDURES Animals All animals including pregnant ICR mice,...
Please summarise the following content:(no word
limit)
EXPERIMENTAL PROCEDURES Animals All animals including pregnant ICR mice, neonatal Wistar rats, and NOD-SCID mice (8 weeks old, male) were purchased from CLEA Japan (Tokyo). All exper- imental procedures and protocols were approved by the Animal Care and Use Committee of Keio University and conformed to the National Institutes of Health Guide for the Care and Use of Laboratory Animals. Cells Mouse ESCs were obtained from the Laboratory of Pluripotent Cell Studies, RIKEN...
Hi everybody,can you help me for problem 4 in the attach . Thank for help! The...
Hi everybody,can you help me for problem 4 in the attach . Thank
for help!
The elementary reversible reaction A + B 2C occurs in the liquid phase in isothermal CSTR. A stream containing equimolar amounts of A and B in a solvent enters. the reactor, and the conversion of A is measured to be 60%. The equilibrium conversion o A under the same inlet conditions is known to be 80%. The volumetric flowrate into the process is to be...
Question 36 1 pts 36. You have a population of mammalian cells growing in media containing enough growth factors. Which of the following graphs corresponds to these cells when their DNA content is analyzed 24 hours after they are induced to express high levels of a mutant form of Rb protein that cannot be phosphorylated? (Note: this cell line has its cell cycle around 22 hours) Control cells #1 #2 #3 #4 #5 #6 Cell number JAN ul. 2 2...
Question 9 1 pts You are given a flask with standard liquid media containing a population of S. pombe cells that have a temperature sensitive mutation of a gene that causes complete loss of function of the gene product at the restrictive temperature. The flask had been incubating at the permissive temperature in the dark before it was given to you, and the cells in the flask were replicating asynchronously with respect to the stage of the cell cycle. The...
Please need help answering question A the pages of background
information are posted thanks
Read page 196-197 and figure 6.20. regarding Meselson and
Stahl’s experiment regarding DNA replication. And Answer the
following question
If you are using this radioactive technique in mouse cells,
what would happen in each phase of G1, S, G2, mitosis and meiosis
assuming that you are grown cells in 15N medium for many
generations and cells in G1are then switched to 14N medium?
G1
S
G2...
Please explain each part !! Thank you !!
#3. Under experimental conditions, you purify a known, biologically important peptide from human tissue. To determine the overall sequence, you then digest the pure peptide with trypsin and chymotrypsin (each separately) and then use mass spectrometry to identify the following sequence fragments from the overall peptide (shown in order of relative length, not necessarily in the order in which they are assembled in the whole, natural peptide). Trypsin cleavage products: Chymotrypsin cleavage...
1. Which of the following statements is FALSE? Helicase activity 'unwinds DNA making the double-stranded molecule into single strands. b. The leading strand of DNA is started by an RNA primer The lagging strand of DNA is synthesized as "Okazaki fragments", cach with its own RNA primer. DNA replication proceeds in both directions around the bacterial chromosome. DNA polymerase synthesives new DNA in one direction (3 to 5) only. 2. Which of the following would be found in eukaryotes? a....
please answer all 5! thank you!
Question 1 1 pts Which of these individuals would be considered a 'mutant'? A person with an XO sex chromosome genotype The recessive allele for a straight hairline in humans A population of sunflowers which produce unique, red/orange petals, native to the southeastern tip of Kansas A turtle carrying an allele enabling it to wield a pair of daggers, present in only 0.0001% of the turtle population. Allele is expressed in dominant form prior...
1. Please provide correct solution. Thank you.
Acid rain is a serious environmental problem. A sample of rainwater collected in the Sierra Mountains had an H+ concentration of 0.1 mmol/L. The pH of this sample was 0 1 3 4 100 Rank the elements carbon (C), hydrogen (H), oxygen (O), and phosphorus (P) in decreasing order number of covalent bonds they usually form. C > P > N > O >H P > O > C > N > H...
2. You are performing PCR under the following conditions: 1. Each PCR reaction contains 50 pl final volume II. Each PCR reaction contains: 1x PCR buffer, 1.5 mM MgCl2, 0.2 mM dNTP mix, 0.2 MM sense primer, 0.2 uM anti-sense primer, 1.5 units of Taq polymerase and 2 ul of DNA template. [all concentrations are final] III. Stock solutions of the following reagents are available: 10x PCR buffer, 25 mm MgCl2, 10 mM dNTP mix, 10 M of sense primer,...
Please summarise the following content:(no word
limit)
EXPERIMENTAL PROCEDURES Animals All animals including pregnant ICR mice, neonatal Wistar rats, and NOD-SCID mice (8 weeks old, male) were purchased from CLEA Japan (Tokyo). All exper- imental procedures and protocols were approved by the Animal Care and Use Committee of Keio University and conformed to the National Institutes of Health Guide for the Care and Use of Laboratory Animals. Cells Mouse ESCs were obtained from the Laboratory of Pluripotent Cell Studies, RIKEN...
Hi everybody,can you help me for problem 4 in the attach . Thank
for help!
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