Question

QUESTION 13 1 points Quiz 5 Extra Credit: Why can scFvs be made efficiently in E.coli? a. They are a single polypeptide chain that lacks posttranslational modification b. They must be heavily glycosylated in the endoplasmic reticulum c. Any IgG can be efficiently made in E.coli d. They are made of multiple polypeptide chains QUESTION 14 1 pc Quiz 5 Extra Credit: Which part of an antibody binds to an antigen? a. Complimentary determining region b. Fc c. Fab Od. Variable domains e. Both (a) and (c) Of. (a), (c) and (d) QUESTION 15 1 points Quiz 5 Extra Credit: What is most likely to happen if you forget the blocking step during an immunoblot? a. Antibodies will bind directly to the membrane b. You will not observe a signal during detection c. Antibodies will no longer bind to epitopes d. Secondary antibodies will be unable to bind primary antibodies

Answer 1

13. A) they are a single polypeptide chain that lacks posttranslational modification. Proteins that do not require posttranslational modification can be efficiently made in prokaryotes since posttranslational modification occurs in eukaryotes.

14. E) Both a & c .

Complementary determining regions (CDRs) are present in the Fab portion of the antibody.

15. C) Antibodies will no longer bind to epitopes. Blocking is performed to block the unnecessary binding of antibody and if blocking is missed then antibody will no longer bind to it's desired epitope hence specificity will be lost.