Question

Lab Assignment Restriction Enzymes Names of restriction enzymes are derived from bacterial genus, specie, strain and ordered in which they were discovered For each of the restriction enzyme below name the bacterial genus and species from which each is derive and is the restriction site that is out by each (show palindrome sequence). Use tablet or laptop to complete assignment (10 pts) 1. E00621 2 EcoRI 14. Styl 3. BamHI 15. Acel 4. Haell 16. Fok! 5. EcoRV 17. Sphi 6. Hhal 18. Batzi 7. Hincli 8 Hindi 9. Hpall 10. Stul 11. Trugl 12. Aatil 13. Sapi

I feel like this is an easy assignment and I am just not grasping the concept

Answer 1

Cut Serial No. Name of Enzyme Eco521 Name of source organism Escherichia coli RFL52 Recognition sequence EcoRI Escherichia coli RY13 5' CGGCCG 3' GCCGGC 5' GAATTC 3' CTTAAG Bacillus amyloliquefaciens H BamHI Bacillus amyloliquefaciens H Haemophilus aegypticus 4 Haell EcoRV Escherichia coli J62 PLG74 5' ---c GGCCG--- 3' 3' ---GCCGG C--- 5' 5' ---G AATTC--- 3' 3' ---CTTAA G--- 5' 5' ---G GATCC--- 3' 3' ---CCTAG G--- 5' 5' ---RGCGC --- 3' 3' ---Y CGCGR--- 5' 5' ---GAT ATC--- 3' 3' ---CTA TAG--- 5' 5' ---GCG C--- 3' 3' --- C GCG--- 5' 5' ---GTY RAC--- 3' 3' ---CAR YTG--- 5' 5' ---A AGCTT--- 3' 3' ---TTCGA A--- 5' 5' ---C CGG--- 3' 3' ---GGC C--- 5' 5' ---AGG CCT--- 3' 3' ---TCC GGA--- 5' Hhal Haemophilus haemolyticus Haemophilus influenzae Rc 5' RGCGCY 3' YCGCGR 5' GATATC 3' CTATAG 5' GCGC 3' CGCG 5' GTYRAC 3' CARYTG 5' AAGCTT 3' TTCGAA 5' CCGG 3' GGCC 5' AGGCCT 3' TCCGGA Hincll Hindlu Haemophilus influenzae Rd Hpall Haemophilus parainfluenzae Stul Streptomyces tubercidicus

Tru91 Thermus ruber 9 5' ---T 3' ---AAT TAA--- 3' T--- 5' 5' TTAA 3' ААТТ 5' GACGTC 3' CTGCAG Aatll Acetobacter aceti 5' ---GACGT C--- 3' 3' ---C TGCAG--- 5' Sspl Sphaerotilus sp. 14 Styl Salmonella typhi Acc! Acinetobacter calcoaceticus Fok! 5' AATATT 3' TTATAA 5' CCWWGG 3' GGWWCC 5' GTMKAC 3' CAKMTG 5' GGATG 3' CCTAC 5' GCATGC 3' CGTACG 5' CGGCCG 3' GCCGGC 5' ---AAT ATT--- 3' 3' ---TTA TAA--- 5' 5' ---C CWWGG--- 3' 3' ---GGWWC C--- 5' 5' ---GT MKAC--- 3' 3' ---CAKM TG--- 5 5' ---GGATGN N NNNN--- 3' 3' ---CCTACN,NNNNN --- 5' 5' ---GCATG C--- 3' 3' ---c GTACG--- 5' 5' ---c GGCCG--- 3' 3' ---GCCGG C--- 5' Flavobacterium okeanokoites Sphl Streptomyces phaeochromogenes Bstzi Bacillus stearothermophilus Z130