During the Homology Directed Repair (HDR), what would happen if we were to provide the yeast cell with a 80bp repair template that contains a mutation in the PAM site?
Homologus directed repair
(HDR) is a mechanism in cells to repair double-strand DNA lesions.
The most common form of HDR is homologous
recombination. The HDR mechanism can only be used by the cell when
there is a homologous piece of DNA present in the
nucleus, mostly in G2 and S phase of the cell cycle.
If a gene is mutated (PAM site) are repaired by certain enzymes
before the gene is expressed. DNA repair is an important process by
which body repairs itself and protects it from disease. i have
attached a photo explaining the repair template that contains a
mutation PAM site of yeast cell.
please do like and comment if you have any query.
During the Homology Directed Repair (HDR), what would happen if we were to provide the yeast...
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This is genetics, please give detailed answers,
QUESTIONS:
1. In looking at the class data, do you think the experiment was
overall successful or not. Describe in a few sentences for your
reasoning.
2. Groups 3 and 4 had lawns of Ade+ yeast suggesting that their
culture was not mutant. Interestingly, group 10 also use a colony
from this same mutant, which they had isolated earlier in the
semester. From group 10’s previous work, this mutant was found to
be...
4. The CRISPR-Cas9 system is an important new technique in
molecular biology. What is the natural function of this system?
Describe how you would use this system to generate a null mutation
in another organism (i.e. explain Figure 6-43). How does it work?
What is the modification of the method that allows for correction
of a mutation (e.g. the mouse crystalline gene)? And lastly, what
are the problems with the CRISPR system?
FIGURE 6-43 Single-nucleotide mutations
can be introduced into...
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