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During the Homology Directed Repair (HDR), what would happen if we were to provide the yeast...

During the Homology Directed Repair (HDR), what would happen if we were to provide the yeast cell with a 80bp repair template that contains a mutation in the PAM site?

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classmate O Date Page Double Strand Break DNA APAM sequence (80bop) 1001101JOJ DOU0117000OOD stripped out Homologus DNA RepaiHomologus directed repair (HDR) is a mechanism in cells to repair double-strand DNA lesions. The most common form of HDR is homologous recombination. The HDR mechanism can only be used by the cell when there is a homologous piece of DNA present in the nucleus, mostly in G2 and S phase of the cell cycle.
If a gene is mutated (PAM site) are repaired by certain enzymes before the gene is expressed. DNA repair is an important process by which body repairs itself and protects it from disease. i have attached a photo explaining the repair template that contains a mutation PAM site of yeast cell.

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