During a PCR, which of the following graphs is associated with (1pt):
The number of primer molecules?
The number of amplicon molecules?
The number of amplicon molecules when only a single primer is used?
The number of Taq polymerase molecules?
The number of primer molecule will decrease- Graph D
The number of amplicons molecules will increase exponentially- Graph B
The number of amplicons molecules will increase in a linear passion when a single primer is used- Graph A
The amount of enzyme remains unchanged in any catalytic reaction- Graph C
During a PCR, which of the following graphs is associated with (1pt): The number of primer...
25. Which is not a required reactant for a PCR reaction? Select one: a. Primer DNA b. Taq polymerase c. dNTP d. template DNA e. ATP
Which of the following molecules is not required for a PCR reaction? View Available Hint(s) Ligase O Primer O DNA O DNTPs Submit Previous Answers X Incorrect; Try Again; One attempt remaining dNTPs are required because they are the building blocks of DNA molecules. - Part B The thermostability of Taq polymerase is required during the annealing phase of PCR. View Available Hint(s) O True O False Submit
1.The PCR (polymerase chain reaction) protocol that is currently used in laboratories was facilitated by the discovery of a bacterium called Thermus aquaticus in a hot spring inside Yellowstone National Park, in Wyoming. This organism contains a heat-stable form of DNA polymerase known as Taq polymerase, which continues to function even after it has been heated to 95°C. a.Why would such a heat-stable polymerase be beneficial in PCR? b.What would happen if it weren’t heat stable? c.How might you choose...
A number of different buffers/components are used in the PCR. Explain the function of each: a. DNA plasmid b. Primer pair C. dNTPs mixture d. Taq polymerase Question 2 (3 points) In our PCR reaction the optimum annealing temperature is 56°C. Explain the general factors that help determine the optimum annealing temperature of a PCR reaction.
1. A number of different buffers/components are used in the PCR. Explain the function of each: a. DNA plasmid b. Primer pair c. dNTPs mixture d. Taq polymerase 2. In our PCR reaction the optimum annealing temperature is 56*C. Explain the general factors that help determine the optimum annealing temperature
2. You are performing PCR under the following conditions: 1. Each PCR reaction contains 50 pl final volume II. Each PCR reaction contains: 1x PCR buffer, 1.5 mM MgCl2, 0.2 mM dNTP mix, 0.2 MM sense primer, 0.2 uM anti-sense primer, 1.5 units of Taq polymerase and 2 ul of DNA template. [all concentrations are final] III. Stock solutions of the following reagents are available: 10x PCR buffer, 25 mm MgCl2, 10 mM dNTP mix, 10 M of sense primer,...
help Which of the following enzyme is essential in PCR? RNA primers taq- polymerase • RNA polymerase reverse transcriptase ORNA dependent RNA polymerase
Lab 1 - PCR Pre-lab discussion. Discuss the following among your table prior to beginning the lab. 1. Describe the overall goal of the polymerase chain reaction. 2. List the components that are required for a PCR reaction. 3. A special DNA polymerase called Taq is used in PCR. What is unique about this enzyme and why is it used in the PCR reaction? 4. We will be using genomic DNA as our template DNA in this PCR reaction. How...
12. During the annealing cycle, if the annealing temperature is too low: a. Very low quantity of amplicon is yielded b. Non specific amplification can occur c. The number of cycles must be redone d. The DNA sample is degraded 13. In PCR cycles, by the end of each cycle, the copy number of amplicon is nearly a Tripled b. Doubled c. Decreased d. Quadrupled 14. PCR amplification is carried out by which of the following instruments: a. Thermal cycler...
5. Which of the following single-stranded DNA sequences is most likely to form a stem-loop structure? a. GACCGTATGCACGGTC b. TCATAGGCGCCGTTCA c. GGATCACGTTACCGCC d. ATAAGATGGGAGCATG The structures below represent the 8 D stereoisomeric aldohexoses. Answer the following (6-7) based on this figure Si carbons ---OH H= =QH --OH H--OH CH,OH HO--- H=CHCH -- - OH CH,OH ---CH HỌC 6 -- - OH Chon D-Glucose HO-- H H -- - OH CH,OH -Marnese -- Hà QH HO--- - OH CHOH Giese HO--...