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Which of the following enzyme is essential in PCR? RNA primers taq- polymerase • RNA polymerase reverse transcriptase ORNA dependent RNA polymerase

Answer 1

Answer- Taq polymerase

Explanation- Polymerase chain reaction was first discovered by Kary Mullis. It involves three steps to amplify the amount of DNA. The three steps are as follows

1) Denaturation of double stranded DNA is done at 95° C . Then unwinded single stranded DNA acts as template.

2) Annealing is done at 55° C. Here in template DNA, primer binds with their complementary sequence.

3) Polymerization is the last step where Taq polymerase is used. The function of this enzyme is to synthesize identical DNA strands as taken. This step is done at 72° C. Taq polymerase is a heat stable enzyme obtained from Thermus aquaticus, found in hot spring. Taq polymerase is a DNA polymerase enzyme.