Question

What is Sanger sequencing, and how does it work with fluorescently labeled primers? Explain the features of your DNA spectra and what parts of the DNA molecule are responsible for these features.

Answer 1

Answer:

1):

SANGER SEQUENCING It is also known as Chan termination method." Method for determining nucleotide sequence of DNA > It is a method of DNA sequencing based on the selective incorporation of chain - teeminating dideoxynucleotides by DNA polymerase during in vitro DNA replication In this sequencing the target DNA is copied many times making flagments of different lights. Fluorescent 66 chain terminator" nucleotides mark the ends of the fragments and allow the Sequence to be determined. - for Two bye methods primer used method DNA and sequencing. dye teeminator method - In oye attached is the nucleotides Primer to the end are method s' end opposite added tluorescent dyes are of the primer, which to that where during chain growth. 5' _ - Labelled prines Template