Question

please check the ans and provide explanation~thx!

f. Prokaryotic RNA polymerase has helicase activity.. Tor Fe Tor Fe g. The 5'methylguanosine cap is linked with phosphate bond at the 5' end of the mRNA in prokaryotes. Telomerase is an enzyme with non-protein component. h. ie Gyrase is a target of some antibiotics." T or F T or Fe Tor Fe j. Poly-U sequence is upstream to the +1 on the DNA template in RNA synthesis.

Answer 1

(f). True - RNA polymerase (RNAP) is an enzyme that synthesizes RNA from a DNA template. RNAP locally opens the double stranded DNA so that one strand of the exposed nucleotides can be used as a template for the synthesis of RNA

(g). True

5' mRNA capping

pppNp 7-methylguanylate phosphohydrolase ABY BA Gppp + ppNp— guanylyl transferase PP OH 5-5'Linkage е ва GpppNp- Base 1 +CH, from S-Ado-Met guanylyl-7-methyl transferase m'GpppNp- Base 2 +CH, from S-Ado-Met | 2-0-methyl transferase mGppoNmp

(h). False - Telomerase is a ribonucleoprotein ( composed of both RNA and proteins) containing the reverse transcriptase telomerase protein (hTERT) and the telomerase RNA template (hTERC) as essential components.

-Telomerase maintains chromosome ends.

(i). True - Bacterial DNA gyrase is the target of many antibiotics, including nalidixic acid, novobiocin and ciprofloxacin

(j).False ( but not sure)