Homework Answers
1. Yes the curve follows Beer's law because as the concentration of glucose in the blood increases, the absorbance value also increases. This clearly shows that the absorption of light by a species is directly proportional to the concentration of absorbing species and this is Beer's law.
2. It goes through origin because whenever the concentration of absorbing species is zero, it means there are no molecules to absorb light. Therefore in the absence of absorbing molecules, the absorbance value will be zero.
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fou will use simulated spectrophotometry to plot a glucose...
Can someone explain to me two possible objectives of this lab? Thank you Part B: Determining...
Can someone explain to me two possible objectives of this lab?
Thank you
Part B: Determining the Concentration of a Solution: Beer's Law The objective of this experiment is to determine the concentration of an unknow sulfate (Ns04) known concentrations (standard solutions) An aliuot of each selution is tranaferred to a small rectangular cuvette that is placed into the solution and etry You are to prepare ive Ni504 solutions of ometer. The amount of Light that ) solution through use...
Please help I am completely confused, I included the 2 graphs needed for this post lab....
Please help I am completely confused, I included the 2
graphs needed for this post lab. Thank you!
Postlab Assignments . (a) Using your own standard curve, the line equation you generated and Beer's law equation Equation 1) calculate the molar absorpivity (e) of blue dye. For all your measurem cuvette used is 1.00 cm. Use this as the value of b in Equation 1 ents, the size of (b) Using Beer's law equation again, what does a high value...
Pls, use Mathlab to answer all questions. Thank you. 2. The energy of the mass-spring system...
Pls, use Mathlab to answer all questions.
Thank you.
2. The energy of the mass-spring system is given by the sum of the kinetic energy and the potential energy. In the absence of damping, the energy is conserved mu2ky2 (a) Add commands to LAB05ex1 to compute and plot the quantity E as a function of time. What do you observe? (pay close attention to the y-axis scale and, if necessary, use ylim to get a better graph). Include at least...
please help with ALL questions thank you 1. Using excel, plot your standard curve based on...
please help with ALL questions thank you
1. Using excel, plot your standard curve based on Table 9-1 and calculate the line of best fit. Put concentration in the x column and OD in the y to solve for the concentrations of your unknown in Table 9-3. Report the slope and the y-intercept below. (1 mark) Slope (m) = v-intercept(b) = 2. Can this standard curve for yeast cells be used for cali cells (why/ why not?) (2 marks) 3....
please complete all the questions thank you Carbohydrates How are carbohydrates classified? What enzymes are required...
please complete all the questions thank you
Carbohydrates How are carbohydrates classified? What enzymes are required for carbohydrate digestion, organ produced in, organ secreted into, and their functions? Know what type of absorption is used for the each monosaccharide. Know what form carbohydrates are absorbed and where they go post absorption. Know the cause and treatment of lactose intolerance. Know the benefits of eating fiber and the food sources of soluble and insoluble fiber, which type is digested by bacteria....
please provide the answer step by step for all the questions. thank you 2. a. was...
please provide the answer step by step for all the
questions. thank you
2. a. was The analysis of the complex formed between Cu(I) and 1,10-phenanthroline conducted using molecular spectroscopic method. The results showed that this complex has a molar absorptivity (8) of 7000 L cm'mol and maximum wavelength absorption (1) of 435 nm. Calculate the absorbance of a 6.77 x 10-6 M solution of the complex when measured in a 1.00 cm cell at wavelength of 435 nm. b....
i need help with all question 5 please! thank you Question 5: Use the data below...
i need help with all question 5 please! thank you
Question 5: Use the data below to construct a Michaelis-Menton curve of velocity vs. [S]. This is quite easy to do in Excel. Vo (UM/s) a) Estimate Vmax from your curve. b) Describe any difficulty you have in completing part (a). Is the enzyme saturated at the highest [S]? c) Using your Vmax estimate, calculate ! Vmax, and using your curve, estimate Km. 1/[S] M 8 1/VO (s/UM) 3.85E-03 [S]...
please answer each in 2-3 sentences. thank you 1. We have learned that energy can exist...
please answer each in 2-3 sentences. thank you
1. We have learned that energy can exist in two primary forms: potential energy and kinetic energy a. Which of the two molecules below contains less potential energy? How do you know? (4 points) CH,OH CH OH —0 OH он H-C-H OH OH b. Glucose is high in potential energy. Why does the cell not simply use glucose to power cellular processes? (2 points) c. Explain how the hydrolysis of ATP can...
help! i'm not sure how to go about this. ABSORBANCE PLOT DATA Please attach a copy...
help! i'm not sure how to go about this.
ABSORBANCE PLOT DATA Please attach a copy of your print out from the spectrophotometer, or indicate that it is attached to your lab partners report and give their name. ron A= emll Analytical wavelength used (=(A-b)lum CALIBRATION CURVE DATA Concentration of Stock Solution: 5.00 X 10 M Door Test Tube Volume Final Volume Final Stock Soln. Solution Concentration 1 2.00 10.0 Absorbance Trial 1 Absorbance Trial 2 2. 4.00 10.0 3...
Where do the numbers come from for a Michaelis-Menton and Lineweaver-Burke Plot? Can you please explain...
Where do the numbers come from for a Michaelis-Menton and
Lineweaver-Burke Plot? Can you please explain in detail please and
thank you?
Did
an Enzyme lab w/dilutions and recorded absorbances. I am now asked
to construct Michaelis-Menton ans Lineweaver-Burke plots BUT I dont
know if the plot is based on my results from the dilutions and
absorbances.
For your lab reports, you will determine how much sugar was made during your enzyme reactions in Week 2 based on the linear...
Can someone explain to me two possible objectives of this lab?
Thank you
Part B: Determining the Concentration of a Solution: Beer's Law The objective of this experiment is to determine the concentration of an unknow sulfate (Ns04) known concentrations (standard solutions) An aliuot of each selution is tranaferred to a small rectangular cuvette that is placed into the solution and etry You are to prepare ive Ni504 solutions of ometer. The amount of Light that ) solution through use...
Please help I am completely confused, I included the 2
graphs needed for this post lab. Thank you!
Postlab Assignments . (a) Using your own standard curve, the line equation you generated and Beer's law equation Equation 1) calculate the molar absorpivity (e) of blue dye. For all your measurem cuvette used is 1.00 cm. Use this as the value of b in Equation 1 ents, the size of (b) Using Beer's law equation again, what does a high value...
Pls, use Mathlab to answer all questions.
Thank you.
2. The energy of the mass-spring system is given by the sum of the kinetic energy and the potential energy. In the absence of damping, the energy is conserved mu2ky2 (a) Add commands to LAB05ex1 to compute and plot the quantity E as a function of time. What do you observe? (pay close attention to the y-axis scale and, if necessary, use ylim to get a better graph). Include at least...
please help with ALL questions thank you
1. Using excel, plot your standard curve based on Table 9-1 and calculate the line of best fit. Put concentration in the x column and OD in the y to solve for the concentrations of your unknown in Table 9-3. Report the slope and the y-intercept below. (1 mark) Slope (m) = v-intercept(b) = 2. Can this standard curve for yeast cells be used for cali cells (why/ why not?) (2 marks) 3....
please complete all the questions thank you
Carbohydrates How are carbohydrates classified? What enzymes are required for carbohydrate digestion, organ produced in, organ secreted into, and their functions? Know what type of absorption is used for the each monosaccharide. Know what form carbohydrates are absorbed and where they go post absorption. Know the cause and treatment of lactose intolerance. Know the benefits of eating fiber and the food sources of soluble and insoluble fiber, which type is digested by bacteria....
please provide the answer step by step for all the
questions. thank you
2. a. was The analysis of the complex formed between Cu(I) and 1,10-phenanthroline conducted using molecular spectroscopic method. The results showed that this complex has a molar absorptivity (8) of 7000 L cm'mol and maximum wavelength absorption (1) of 435 nm. Calculate the absorbance of a 6.77 x 10-6 M solution of the complex when measured in a 1.00 cm cell at wavelength of 435 nm. b....
i need help with all question 5 please! thank you
Question 5: Use the data below to construct a Michaelis-Menton curve of velocity vs. [S]. This is quite easy to do in Excel. Vo (UM/s) a) Estimate Vmax from your curve. b) Describe any difficulty you have in completing part (a). Is the enzyme saturated at the highest [S]? c) Using your Vmax estimate, calculate ! Vmax, and using your curve, estimate Km. 1/[S] M 8 1/VO (s/UM) 3.85E-03 [S]...
please answer each in 2-3 sentences. thank you
1. We have learned that energy can exist in two primary forms: potential energy and kinetic energy a. Which of the two molecules below contains less potential energy? How do you know? (4 points) CH,OH CH OH —0 OH он H-C-H OH OH b. Glucose is high in potential energy. Why does the cell not simply use glucose to power cellular processes? (2 points) c. Explain how the hydrolysis of ATP can...
help! i'm not sure how to go about this.
ABSORBANCE PLOT DATA Please attach a copy of your print out from the spectrophotometer, or indicate that it is attached to your lab partners report and give their name. ron A= emll Analytical wavelength used (=(A-b)lum CALIBRATION CURVE DATA Concentration of Stock Solution: 5.00 X 10 M Door Test Tube Volume Final Volume Final Stock Soln. Solution Concentration 1 2.00 10.0 Absorbance Trial 1 Absorbance Trial 2 2. 4.00 10.0 3...
Where do the numbers come from for a Michaelis-Menton and
Lineweaver-Burke Plot? Can you please explain in detail please and
thank you?
Did
an Enzyme lab w/dilutions and recorded absorbances. I am now asked
to construct Michaelis-Menton ans Lineweaver-Burke plots BUT I dont
know if the plot is based on my results from the dilutions and
absorbances.
For your lab reports, you will determine how much sugar was made during your enzyme reactions in Week 2 based on the linear...
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