Question

Shotgun sequencing Question 6 Not yet answered Points out of 2.00 P Flag question Select one: O a. involves assembling randomly-sequenced clones by computer b. is not well suited for genomes that contain many repetitive sequences C. does not involve assembling clones to make a physical map d. is best suited for small genomes O e. all of these are correct During RNA interference: Question 7 Not yet answered Points out of 2.00 Flag question Select one: o a. double stranded RNA is recognized by Dicer and cut into siRNA O b. RISC removes one strand of siRNA and helps it bind to its complementary mRNA o c. DNAs containing most genes in the genome are deposited on a small glass chip O d. two of these answers are correct e. all of these answers are correct

Answer 1

1. a. shotgun sequencing is a technique for determining the sequence of entire genome based on producing random DNA fragments that assembled by computers that make the order of fragment by finding the overlapping ends.

2. c. two of these statements are correct i.e. a and b.

RNA interference mechanism

double stranded RNA bind to protein dicer which cleaves dsRNA into small fragments. one of RNA strands is loaded into RISC complex and links the complex to mRNA strand by base pairing mRNA cleaved  and destroyed. No protein can be synthesized.