Homework Answers
Answer - It has an ER signal sequence and a Mannose 6 phosphate site
ER signal sequence is located at the N-terminus and are cleaved from the protein in the rough ER lumen. ER serves multiple functions mportant particularly in the synthesis, folding, modification, and transport of proteins.
Mannose 6 phosphate is a sequence that plays an important role in transporting proteins from the Golgi complex and cell surface to the lysosome.
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A protein sits in a lysosomal vesicle. How could it have ended up there? O MORE...
A protein that has an internal ER-signal sequence and a nuclear localization might end up in...
A protein that has an internal ER-signal sequence and a nuclear localization might end up in the extracellular matrix. in the cytosol. inside a lysosome. o in the plasma membrane.
Help please. Thanks 14. Adenylyl cyclase produces the intracellular signal: a) Kinase b) Phosphatase c) Cyclic...
Help please. Thanks
14. Adenylyl cyclase produces the intracellular signal: a) Kinase b) Phosphatase c) Cyclic AMP d) AMP 15. When adrenalin is produced PKA is turned off a) b) PKA is turned on c) Has no effect on PKA d) None of the above 16. All proteins with a signal sequence enter the ER 17. The sorting signal, Nuclear Localization Sequence, all proteins in the cytosol to enter the 18. In order for proteins to enter the mitochondria, they...
Scientists want to design fluorescent marker proteins for different cellular compartments using the Green Fluorescent Protein...
Scientists want to design fluorescent marker proteins for different cellular compartments using the Green Fluorescent Protein (GFP). This means that the GFP will be targeted and glow at the organelle of interest and they can use these to mark different organelles during fluorescence microscopy. To make these marker proteins, they take the sequence of GFP and add the desired sorting signal. A. GFP on its own is an excellent protein to use as an organelle marker protein because it is...
Please answer all questions 2 After isolating the rough endoplasmic reticulum from the rest of the...
Please answer all questions
2 After isolating the rough endoplasmic reticulum from the rest of the cytoplasm, you purify the RNAS attached to it. Which of the following proteins do you expect the RNA from the rough endoplasmic reticulum to encode? (a) (c) soluble secreted proteins plasma membrane proteins ER membrane proteins all of the above (b) (d) -13 In which cellular location would you expect to find ribosomes translating MRNAS that encode ribosomal proteins? (a) (c) the nucleus in...
In-Class Activity #12, Protein Tratlicking Worksheet 1. You are interested in four different proteins in a...
In-Class Activity #12, Protein Tratlicking Worksheet 1. You are interested in four different proteins in a yeast cell: • protein 1 is a cytosolic protein protein 2 is a secreted protein • protein 3 is a nuclear protein protein 4 is a cell surface membrane protein with N-terminal end in extra-cellular space. a. You plan to study how the proteins are localized to their specific destination by creating the following mutations in the genes encoding proteins 1-4. Indicate how the...
please answer all that you can 1. You have genetically engineered green fluorescent protein (GFP) containing...
please answer all that you can 1. You have genetically engineered green fluorescent protein (GFP) containing a KDEL sequence (GFP-KDEL). When GFP-KDEL is expressed in normal human fibroblasts and examined using fluorescence microscopy, the fluorescence appears diffuse across the cytoplasm. How would you explain this observations given that KDEL is supposed to be an ER-specific sorting sequence? A. This engineered GFP would not have a hydrophobic signal sequence to get it into the RER in the first place. B. The...
Name 2ors L! You are inte finding protein are interested in understanding how Top5 expression is...
Name 2ors L! You are inte finding protein are interested in understanding how Top5 expression is regulated. Top5 is a microtubule protein. You study a cell line where Top5 expression is controlled by a signal, Ligl. When hinds to and activates the membrane bound recentor it activates the Ras protein, which activates atein kinase called PKF, which then phosphorylates Grp4 on T224. Grp4 is a gene regulatory Wotein that can only enter the nucleus when phosphorylated: Thos is not expressed...
G alpha z (Gaz) is a protein of 355 amino acids in length. It is synthesized...
G alpha z (Gaz) is a protein of 355 amino acids in length. It is synthesized in the cytoplasm and has no signal sequence, nor a start-transfer sequence, nor a nuclear import sequence or patch. After its initial synthesis, Gaz is bound by another protein called a palmitoyltransferase, which covalently attaches a fatty acid called palmitate to a cysteine side chain near the N-terminus of Gaz. This lipid modification allows Gaz to associate with the inner face of the plasma...
please answer me question ( a) and (b) in no more than 6 sentences. thanks. How...
please answer me question ( a) and (b) in no more than 6
sentences. thanks.
How could you design a drug to target nuclear import that could prevent HIV infection? How might your design impact the normal function of cells, leading to side effects? Importin and Exportin also bind to Ran, a GTPase, which can be bound to GTP or GDP Ran-GDP GDP Ran-GAP CYTOSOL A concentration gradient is formed of RanGTP inside the nucleus and RanGDP outside the nucleus...
Of: a) NA as the site s eukaryotic cells become more specialized, the nucleus is NOT important sy...
PLEASE ANSWER ALL
of: a) NA as the site s eukaryotic cells become more specialized, the nucleus is NOT important synthesis. b) Changes in the regulation of genes, c) DNA mutation, d) synthesis of mRNA, e) Golgi apparatus synthesis 4. Which of the following is associated with Co-enzyme A by the membrane of the mitochondrion for use in the Krebs cycle? a) proteins, b) amino acids, c) fatty acids, d) pyruvate e) glycogen 5. Which is NOT a characteristic of...
A protein that has an internal ER-signal sequence and a nuclear localization might end up in the extracellular matrix. in the cytosol. inside a lysosome. o in the plasma membrane.
Help please. Thanks
14. Adenylyl cyclase produces the intracellular signal: a) Kinase b) Phosphatase c) Cyclic AMP d) AMP 15. When adrenalin is produced PKA is turned off a) b) PKA is turned on c) Has no effect on PKA d) None of the above 16. All proteins with a signal sequence enter the ER 17. The sorting signal, Nuclear Localization Sequence, all proteins in the cytosol to enter the 18. In order for proteins to enter the mitochondria, they...
Scientists want to design fluorescent marker proteins for different cellular compartments using the Green Fluorescent Protein (GFP). This means that the GFP will be targeted and glow at the organelle of interest and they can use these to mark different organelles during fluorescence microscopy. To make these marker proteins, they take the sequence of GFP and add the desired sorting signal. A. GFP on its own is an excellent protein to use as an organelle marker protein because it is...
Please answer all questions
2 After isolating the rough endoplasmic reticulum from the rest of the cytoplasm, you purify the RNAS attached to it. Which of the following proteins do you expect the RNA from the rough endoplasmic reticulum to encode? (a) (c) soluble secreted proteins plasma membrane proteins ER membrane proteins all of the above (b) (d) -13 In which cellular location would you expect to find ribosomes translating MRNAS that encode ribosomal proteins? (a) (c) the nucleus in...
In-Class Activity #12, Protein Tratlicking Worksheet 1. You are interested in four different proteins in a yeast cell: • protein 1 is a cytosolic protein protein 2 is a secreted protein • protein 3 is a nuclear protein protein 4 is a cell surface membrane protein with N-terminal end in extra-cellular space. a. You plan to study how the proteins are localized to their specific destination by creating the following mutations in the genes encoding proteins 1-4. Indicate how the...
Name 2ors L! You are inte finding protein are interested in understanding how Top5 expression is regulated. Top5 is a microtubule protein. You study a cell line where Top5 expression is controlled by a signal, Ligl. When hinds to and activates the membrane bound recentor it activates the Ras protein, which activates atein kinase called PKF, which then phosphorylates Grp4 on T224. Grp4 is a gene regulatory Wotein that can only enter the nucleus when phosphorylated: Thos is not expressed...
please answer me question ( a) and (b) in no more than 6
sentences. thanks.
How could you design a drug to target nuclear import that could prevent HIV infection? How might your design impact the normal function of cells, leading to side effects? Importin and Exportin also bind to Ran, a GTPase, which can be bound to GTP or GDP Ran-GDP GDP Ran-GAP CYTOSOL A concentration gradient is formed of RanGTP inside the nucleus and RanGDP outside the nucleus...
PLEASE ANSWER ALL
of: a) NA as the site s eukaryotic cells become more specialized, the nucleus is NOT important synthesis. b) Changes in the regulation of genes, c) DNA mutation, d) synthesis of mRNA, e) Golgi apparatus synthesis 4. Which of the following is associated with Co-enzyme A by the membrane of the mitochondrion for use in the Krebs cycle? a) proteins, b) amino acids, c) fatty acids, d) pyruvate e) glycogen 5. Which is NOT a characteristic of...
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