A protein that has an internal ER-signal sequence and a nuclear localization might end up in the plasma membrane. Translation of all proteins in a eukaryotic cell begins in the cytosol. As a protein is made, it passes step by step through a shipping "decision tree". At each stage, the protein is checked for molecular tags to see if it needs to be re-routed to a different pathway or destination.
The first major branch point comes shortly after translation starts. At this point, the protein will either remain in the cytosol for the rest of translation, or be fed into the plasma membrane as it is translated. Proteins are fed into the plasma membrane during translation if they have an amino sequence called a signal peptide.
A protein that has an internal ER-signal sequence and a nuclear localization might end up in...
The ER signal sequence on a growing polypeptide chain is recognized by a signal-recognition particle (SRP) in the cytosol. What does this interaction accomplish? It cleaves the ER signal sequence from the polypeptide chain. It cleaves the ER signal sequence from the polypeptide chain. It guides the ribosome and its polypeptide to the ER. It releases the polypeptide chain from the ribosome. It speeds the synthesis of the polypeptide chain. Which of the following choices reflects the appropriate order of...
A protein sits in a lysosomal vesicle. How could it have ended up there? O MORE THAN ONE OPTION IS POSSIBLE It has a nuclear localization sequence It has a mitochondrial matrix targeting sequence It has an ER signal sequence and a Mannose-6-phosphate site It was endocytosed from the plasma membrane
If you deleted the ER N-terminal signal sequence from a gene that encodes a protein, where would the protein end up after synthesis? (Assume no other signal sequences are present) The golgi The smooth ER The protein would be secreted from the cell The cytosol The lysosome
For proteins that normally are nuclear, deletion of the nuclear localization signal and injection of the protein into the cytoplasm of cultured cells will result in localization A. that is extracellular. B. in a ring around the outside of the nuclear envelope. C. in the nucleus. D. in the cytoplasm.
Problem 4.1.3 (tags: #protein targeting, #protein topology, #transfer sequence) a) Make a map showing the location of signal sequences, start transfer sequences and stop transfer sequences in protein A and protein B below that end up inserted in the membrane as shown. Protein A NH, Cytosol NA J B Scoot & Membrane Protein B COOH HAN Ν - FC ER Cisterna b) Make a map for the arrangement of signal sequences, start transfer sequences and stop transfer sequences for a...
What happens to a protein that doesn't have a signal sequence? A. It is secreted from the cell. B. It remains in the cytosol C. It gets degraded in the lysosome. D. It becomes a plasma membrane protein.
24.Consider a transcription regulatory protein that has both a nuclear localization and a nuclear export signal and is normally found both in the nucleus and in the cytosol at comparable concentrations. This protein has a high-affinity binding partner in the nucleus. Upon activation of a certain signaling pathway, the binding partner is ubiquitylated and degraded. As a result of this,... the transcription regulatory protein accumulates in the nucleus. the transcription regulatory protein accumulates in the cytosol. expression of its target...
Help please. Thanks
14. Adenylyl cyclase produces the intracellular signal: a) Kinase b) Phosphatase c) Cyclic AMP d) AMP 15. When adrenalin is produced PKA is turned off a) b) PKA is turned on c) Has no effect on PKA d) None of the above 16. All proteins with a signal sequence enter the ER 17. The sorting signal, Nuclear Localization Sequence, all proteins in the cytosol to enter the 18. In order for proteins to enter the mitochondria, they...
Answer and explain why
27) Consider a protein that contains an ER signal sequence at its N-ter- minus and a nuclear localization se- quence in its middle. What do you think the fate of this protein would be? Briefly explain your answer
3) A protein contains an N-terminal cleavable signal sequence and two further transmembrane domains. How is the protein most likely oriented with respect to the ER membrane? How is it oriented when introduced to the plasma membrane after trafficking?