Homework Answers
1) In the uncut lane, Au has 3 bands (one being the intense) and PU has 2 bands (one being the intense). If there are more than one band, one possibility is- it is a plasmid DNA. Plasmid dna runs with multiple bands- nicked, linear and supercoilled.
2) The uncut DNA is electrophoresed because it serves as a control when you run a cut DNA with enzyme on a gel
3) When the know sample of pBR322 was cut with enzyme 1 it showed multiple cuts- 3, 2 and 1.3 Kb while when it was cut with enzyme 2, it did not show any cut.
4) The sizes of the band I can actually see when pBR322 is cut with enzyme 1- 3, 2.6, 2 amd 1.3 Kb
5) The anamolous band might be 3 and 2.6 Kb fragment which has arisen from uncut plasmid.
6) Yes the plasmid which is isolated is pBR322 because the unknown DNA shows same band pattern when cut with enzyme 1 and 2 except the 3 and 2.6 Kb band which might be a result of uncut or partially cut.
Add Answer to:
A1
is my DNA, Enzyme 1 BsrB1
P1 is known pBR322 enzyme 1
A2 is my...
RESULTS: Analyzing your data Answer the following questions: 1) How many bands do you see in...
RESULTS: Analyzing your data Answer the following questions: 1) How many bands do you see in the "unculanes? If there are more than one bad explain the nature or origin of the other bands? If there are more than one band can you 2) Why did you have to electrophorese uncut DNA? 3) What size of fragments were you expecting when you digested the known samples of pBR322 with the enzymes you used? 4) What are the sizes of the...
Can you answer these questions pleasee: If we performed electrophoretic separation (AGE) of human genomic DNA...
Can you answer these questions
pleasee: If we performed electrophoretic separation (AGE) of human
genomic DNA (buccal swab extraction), and we didn’t perform any
restriction digestion, answer the following:
a)In your opinion, which of the loaded samples has the highest
and lowest concentration of DNA, respectively?
b)Indicate the lanes in which DNA is degraded and explain your
answer.
c)Take a look into the lane “cut Plasmid”. Explain the appearance
of two bands. What kind of a technique was performed on...
Prac result Nhel Kpn I 1569 1601 Kpni 1030 2295 / 2310 Not I 988 ОТС-д...
Prac result
Nhel Kpn I 1569 1601 Kpni 1030 2295 / 2310 Not I 988 ОТС-д Sphi 2559 Nde I 484 POTC-A 6401 bp Hygro Amp Nde1 3369 pUC Ori 5260 4587 Amp: Ampicillin resistance gene 5405 - 6265 Hygro: Hygromycin resistance gene Choose the gel image with correct bands in lanes 1 to 5 to indicate the approximate positions where you expect to see these predicted DNA fragments. (1 mark) Lane 1: POTC (undigested / uncut) Lane 2: POTC-A...
The PCR was a success and your target region of 770 bp in length has been...
The PCR was a success and your target region of 770 bp in length has been amplified. You now plan to digest the DNA amplicon with the restriction enzyme Eael, and clone the resulting longest fragment it into the Eael site of the 5 kb plasmid diagrammed below. 770 bp BamHI 1 200 EcoRI 800 EcoRI 4000 1000 5 kb O /1000 2000 2000 Faal You purify your recombinant plasmid from bacterial cells, and run the plasmid (uncut. or not...
1) Imagine that you had two restriction enzymes and a known segment of linear DNA 160...
1) Imagine that you had two restriction enzymes and a known segment of linear DNA 160 kb long: Enzyme A cuts at location(s): 20 kb, 45 kb 70 kb and 110 kb. Enzyme B cuts at location(s): 15 kb and 140 kb. Based on this information, first construct a linear map of this DNA showing the positions of these restriction enzyme cut sites. In the space below.draw what the gel would look like given the following lanes: (8 points total)...
I need the answers for questions 2 and 3. My DNA ladder is in lane 2...
I
need the answers for questions 2 and 3. My DNA ladder is in lane 2
marked by the yellow arrow. Thanks!
Here is the only other info I have. Thanks!
Part 2: Gel purification and on Gel Slice and PCR Product Preparin modified from TBSci.com instructions for gaan A. Dissolving the Gel Stie Following electrophores, eral DNA band from grand place glice microcentrifuge tube Ib. Use an analytical balance to weigh pelice Rec die 2. Add 500 balance to...
I just need the answers to questions 2 and 3. My DNA ladder is in lane...
I just need the answers to questions 2 and 3. My DNA ladder is
in lane 2 with the yellow arrow pointing to it. Thanks!
Part 2: Gel purification and ration Gel Slice and PCR Product Preparation modified from IBSci.com instructions for gel and PCR clean-up system A. Dissolving the Gel Slice 1. Following electrophoresis, excise DNA band from gel and place gel slice in a 1.5ml microcentrifuge tube. 1b. Use an analytical balance to weigh gel slice. Record weight...
very lost on this whole worksheet and i dont know if my current answers are even...
very lost on this whole worksheet and i dont know if my
current answers are even right, please help i need this done by
today
Name: Cloning Worksheet chromosome qut 756bp pBiochem 5.983 bp ladder Lane 1 Lane 2 Lane 3 Lane 4 Lane 5 Lane 6 BamHI (4233) (230) IT Enzyme recognition S ..GATATC. 51 EcoRV S .. . 3..CTATAC... 5 12 Pvull 89 BamHI Pett You are given the task to clone yfg into the plasmid pBioChem. Follow...
help with questions 5 to 10 please PCB 3023L Lab #4 Protocol & Worksheet (30pt) You...
help with questions 5 to 10 please
PCB 3023L Lab #4 Protocol & Worksheet (30pt) You may work in your lab groups durine class. but all written answers must be completed individually in your own words. 1) Using the plasmid map for orientation 1 and the cDNA map as a guide, complete the plasmid map for orientation #2. (4pt) 612 1318 1 - EcoRi EcoRI Xbal ECORV -Xbal- 1662 +Bell EcoRI EcoRV Not FP -- Xhol X 2015 PRSP +...
Chromosomal and plasmid DNA can be cut into manageable pieces by restriction enzymes. Using agarose gel...
Chromosomal and plasmid DNA can be cut into manageable pieces by
restriction enzymes. Using agarose gel electrophoresis, the DNA
fragments can be separated on a gel, based on their lengths. In
order to see the fragments, a stain is typically added to the gel.
The size of each fragment can be determined by comparing each one
to a DNA molecular weight marker of known size.
Below is a map of pBR22 plasmid. The position and base pair
number of the...
RESULTS: Analyzing your data Answer the following questions: 1) How many bands do you see in the "unculanes? If there are more than one bad explain the nature or origin of the other bands? If there are more than one band can you 2) Why did you have to electrophorese uncut DNA? 3) What size of fragments were you expecting when you digested the known samples of pBR322 with the enzymes you used? 4) What are the sizes of the...
Can you answer these questions
pleasee: If we performed electrophoretic separation (AGE) of human
genomic DNA (buccal swab extraction), and we didn’t perform any
restriction digestion, answer the following:
a)In your opinion, which of the loaded samples has the highest
and lowest concentration of DNA, respectively?
b)Indicate the lanes in which DNA is degraded and explain your
answer.
c)Take a look into the lane “cut Plasmid”. Explain the appearance
of two bands. What kind of a technique was performed on...
Prac result
Nhel Kpn I 1569 1601 Kpni 1030 2295 / 2310 Not I 988 ОТС-д Sphi 2559 Nde I 484 POTC-A 6401 bp Hygro Amp Nde1 3369 pUC Ori 5260 4587 Amp: Ampicillin resistance gene 5405 - 6265 Hygro: Hygromycin resistance gene Choose the gel image with correct bands in lanes 1 to 5 to indicate the approximate positions where you expect to see these predicted DNA fragments. (1 mark) Lane 1: POTC (undigested / uncut) Lane 2: POTC-A...
The PCR was a success and your target region of 770 bp in length has been amplified. You now plan to digest the DNA amplicon with the restriction enzyme Eael, and clone the resulting longest fragment it into the Eael site of the 5 kb plasmid diagrammed below. 770 bp BamHI 1 200 EcoRI 800 EcoRI 4000 1000 5 kb O /1000 2000 2000 Faal You purify your recombinant plasmid from bacterial cells, and run the plasmid (uncut. or not...
1) Imagine that you had two restriction enzymes and a known segment of linear DNA 160 kb long: Enzyme A cuts at location(s): 20 kb, 45 kb 70 kb and 110 kb. Enzyme B cuts at location(s): 15 kb and 140 kb. Based on this information, first construct a linear map of this DNA showing the positions of these restriction enzyme cut sites. In the space below.draw what the gel would look like given the following lanes: (8 points total)...
I
need the answers for questions 2 and 3. My DNA ladder is in lane 2
marked by the yellow arrow. Thanks!
Here is the only other info I have. Thanks!
Part 2: Gel purification and on Gel Slice and PCR Product Preparin modified from TBSci.com instructions for gaan A. Dissolving the Gel Stie Following electrophores, eral DNA band from grand place glice microcentrifuge tube Ib. Use an analytical balance to weigh pelice Rec die 2. Add 500 balance to...
I just need the answers to questions 2 and 3. My DNA ladder is
in lane 2 with the yellow arrow pointing to it. Thanks!
Part 2: Gel purification and ration Gel Slice and PCR Product Preparation modified from IBSci.com instructions for gel and PCR clean-up system A. Dissolving the Gel Slice 1. Following electrophoresis, excise DNA band from gel and place gel slice in a 1.5ml microcentrifuge tube. 1b. Use an analytical balance to weigh gel slice. Record weight...
very lost on this whole worksheet and i dont know if my
current answers are even right, please help i need this done by
today
Name: Cloning Worksheet chromosome qut 756bp pBiochem 5.983 bp ladder Lane 1 Lane 2 Lane 3 Lane 4 Lane 5 Lane 6 BamHI (4233) (230) IT Enzyme recognition S ..GATATC. 51 EcoRV S .. . 3..CTATAC... 5 12 Pvull 89 BamHI Pett You are given the task to clone yfg into the plasmid pBioChem. Follow...
help with questions 5 to 10 please
PCB 3023L Lab #4 Protocol & Worksheet (30pt) You may work in your lab groups durine class. but all written answers must be completed individually in your own words. 1) Using the plasmid map for orientation 1 and the cDNA map as a guide, complete the plasmid map for orientation #2. (4pt) 612 1318 1 - EcoRi EcoRI Xbal ECORV -Xbal- 1662 +Bell EcoRI EcoRV Not FP -- Xhol X 2015 PRSP +...
Chromosomal and plasmid DNA can be cut into manageable pieces by
restriction enzymes. Using agarose gel electrophoresis, the DNA
fragments can be separated on a gel, based on their lengths. In
order to see the fragments, a stain is typically added to the gel.
The size of each fragment can be determined by comparing each one
to a DNA molecular weight marker of known size.
Below is a map of pBR22 plasmid. The position and base pair
number of the...
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