"You wish to amplify the region of DNA between the bolded nucleotides below. Determine the REVERSE...
"You wish to amplify the region of DNA between the bolded nucleotides below. Determine the REVERSE primer that you will use for PCR. Write the primer sequence 5` to 3` with no spaces. Make the primer 10 nucleotides long. 5` AGTCA CGATG TTCGC TAGAT AGCTC GATAT ATTTG CCGAT CGCCT 3`
Homework Answers
Assuming you have provided only the bolded nucleotides here, as in your question the nucleotides are not bolded.
For the given above seuence the reverse primer is as follows:
5` AGTCA CGATG TTCGC TAGAT AGCTC GATAT ATTTG CCGAT CGCCT 3'
last ten nucleotides TGCCGATCGCCT
complement sequence: ACGGCTAGCGGA
Reverse primer is as follows 5' AGGCGATCGGCA 3' (reverse complement)
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"You wish to amplify the region of DNA between the bolded
nucleotides below. Determine the REVERSE...
You want to amplify the DNA between the two stretches of sequence shown below. Of the...
You want to amplify the DNA between the two stretches of sequence shown below. Of the listed primer pairs, choose the correct pair that will allow you to amplify the DNA by PCR. 5'-ACATTACCAA----GACCTGCTTT-3' 3' -TGTAATGGTT----CTGGACGAAA-5' 5' -TGTAATGGTT-3' and 5'-TTTCGTCCAG-3' O 5'-ACATTACCAA-3' and 5'-AAAGCAGGTC-3' 5'-TGTAATGGTT-3' and 5'-CTGGACGAAA-3' O 5'-AACCATTACA-3' and 5'-CTGGACGAAA-3'
You want to amplify the DNA between the two stretches of sequence shown below. Of the...
You want to amplify the DNA between the two stretches of sequence shown below. Of the listed primer pairs, choose the correct pair that will allow you to amplify the DNA by PCR. 5’-CTGGACGAAA----TGTAATGGTT-3’ 3’-GACCTGCTTT----ACATTACCAA-5’ A. 5’-CTGGACGAAA-3’ and 5’-TGTAATGGTT-3’ B. 5’-CTGGACGAAA-3’ and 5’-AACCATTACA-3’ C. 5’-TTTCGTCCAG-3’ and 5’-TGTAATGGTT-3’ D. 5’-GACCTGCTTT-3’ and 5’-ACATTACCAA-3’
need help with this Tm: Tm: 16. You wish to amplify the DNA fragments below and...
need help with this
Tm: Tm: 16. You wish to amplify the DNA fragments below and add cut sites for subsequent cloning. Design both primers with the restriction enzymes cut sites listed, and add two overhang bases to each primer. Label the extra bases and cut sites. Each primer should be 14 nucleotides in length. a. 5'-CTATGAGGTCCTGCGTTAGTGTTACC-3' Forward: 5'- 5' EcoRI, 3' BamHI, overhang bases Reverse: 5'- Annealing Temperature: b. 5'- CCTGGGTGTTACATGCCATTAGCGTT-3' Forward: 5'- Tm: 5' HindiII, 3' Sphl, overhang...
You want to use PCR to amplify the entire DNA shown in the figure below. Of...
You want to use PCR to amplify the entire DNA shown in the figure below. Of the listed primers, choose the pair that will allow you to amplify this stretch of DNA by PCR. (9 points total) DNA to be amplified 5’-CGATTCGAGCCATTCGAACTCGATCAGCCGATTCGATCAACCTTGGACAGTCAG-3’ 3’-GCTAAGCTCGGTAAGCTTGAGCTAGTCGGCTAAGCTAGTTGGAACCTGTCAGTC-5’ Primers: (1) 5’-GCTAAGCTCGGTA-3’ (5) 5’-CTTGGACAGTCAG-3’ (2) 5’-GAACCTGTCAGTC-3’ (6) 5’-CGATTCGAGCCAT-3’ (3) 5’-CTGACTGTCCAAG-3’ (7) 5’-ATGGCTCGAATCG-3’ (4) 5’-GACTGACAGGTTC-3’ (8) 5’-TACCGAGCTTAGC-3’ Answer: I will need primer number _____ and primer number _____
pls help The DNA sequence below is 300 bases long. This is only one strand of...
pls help
The DNA sequence below is 300 bases long. This is only one strand of DNA going from 5' starting at base 1081 to 3' ending at base 1380. The complementary strand is NOT shown. The sequence is broken up into 10 base sections to make counting easier. Design primers to amplify a DNA fragment that is 150bps in length. 1081 cagtatcagg tggtggcccc ttgcccccag tcagcaccct gacatcactg cacagtctgt 1141 ctgcctcgcc tgctccccac catggactca toatgacctc cctgcccagc gtcatgagtc 1201 tgggagagtc ctctctcctc ataggtcaaa ccgtacctgt...
1. Create the primers to amplify the gene of interest. 1. Below is almost the full...
1. Create the primers to amplify the gene of interest. 1. Below is almost the full sequence of the coding strand of the F9 gene (exon 2-exon4) that you found in the online database (NCBI, Genomic sequence F9 gene). Some of the sequence is missing, but you know how many nucleotides are skipped. Design forward and reverse primers 18nt each for PCR that will isolate EXACTLY the sequence that is in bold typeface. Exon 2 = 164 nt Intron 2...
S-CGT-3 GTG 3. Write in the following sequences to depict them hybridizing/annealing to complementary and antiparallel...
S-CGT-3 GTG 3. Write in the following sequences to depict them hybridizing/annealing to complementary and antiparallel sequence in the exposed nucleotide chains. 5'-GTG-3 5-CGT-3 5'-ACG-3' | 5 -CAC-3" 5'-AAT[CGTATCAGCAGCAGTG|ACT-3 -3'-TTALGCATAGTCGTCGTCATGA-5'- 3. Two of the four above sequences can be used together as a "primer pair" to PCR amplify the bracketed sequence. In order to determine which two will work, recall that new polynucleotide chains can only be added to on the 3'end. Draw an arrow from the 3' end of...
1. Explain why, when PCR is used to amplify the same region of DNA from two...
1. Explain why, when PCR is used to amplify the same region of DNA from two different people, the size of the DNA fragment(s) generated may be different? 2. What characteristic of the DNA molecule makes it possible to use electrophoresis to separate DNA molecules by size? Explain why this characteristic is important for electrophoresis and what part of the DNA molecule creates this characteristic. 3. You are performing PCR. After four cycles of PCR, how many double-stranded copies of...
2. Design a primer pair (5 nucleotides each) to amplify only exon 2 of the gene...
2. Design a primer pair (5 nucleotides each) to amplify only exon 2 of the gene model below. Use the primer pair you designed to model 3 cycles of DNA replication. 5..CGTGACTGATCATGGAGCTACGATCGACTCTCGAAGCTCTAGACGCTCTCGAGAGCTCTCGAATTAGATCGTAATTGCGGTTCA...3'
The Psilotum nudum genome is 2.5 x 1011 bp. You wish to design a primer to...
The Psilotum nudum genome is 2.5 x 1011 bp. You wish to design a primer to amplify a specific gene in the genome. - Part A In general, what length of oligonucleotide would be sufficient to amplify a single unique sequence? To simplify your calculation, assume that all bases occur with an equal frequency. Hint: The frequency of occurrence of a PCR primer in genomic DNA is given by this equation: genomic DNA length x sequence frequency The sequency frequency...
You want to amplify the DNA between the two stretches of sequence shown below. Of the listed primer pairs, choose the correct pair that will allow you to amplify the DNA by PCR. 5'-ACATTACCAA----GACCTGCTTT-3' 3' -TGTAATGGTT----CTGGACGAAA-5' 5' -TGTAATGGTT-3' and 5'-TTTCGTCCAG-3' O 5'-ACATTACCAA-3' and 5'-AAAGCAGGTC-3' 5'-TGTAATGGTT-3' and 5'-CTGGACGAAA-3' O 5'-AACCATTACA-3' and 5'-CTGGACGAAA-3'
need help with this
Tm: Tm: 16. You wish to amplify the DNA fragments below and add cut sites for subsequent cloning. Design both primers with the restriction enzymes cut sites listed, and add two overhang bases to each primer. Label the extra bases and cut sites. Each primer should be 14 nucleotides in length. a. 5'-CTATGAGGTCCTGCGTTAGTGTTACC-3' Forward: 5'- 5' EcoRI, 3' BamHI, overhang bases Reverse: 5'- Annealing Temperature: b. 5'- CCTGGGTGTTACATGCCATTAGCGTT-3' Forward: 5'- Tm: 5' HindiII, 3' Sphl, overhang...
pls help
The DNA sequence below is 300 bases long. This is only one strand of DNA going from 5' starting at base 1081 to 3' ending at base 1380. The complementary strand is NOT shown. The sequence is broken up into 10 base sections to make counting easier. Design primers to amplify a DNA fragment that is 150bps in length. 1081 cagtatcagg tggtggcccc ttgcccccag tcagcaccct gacatcactg cacagtctgt 1141 ctgcctcgcc tgctccccac catggactca toatgacctc cctgcccagc gtcatgagtc 1201 tgggagagtc ctctctcctc ataggtcaaa ccgtacctgt...
1. Create the primers to amplify the gene of interest. 1. Below is almost the full sequence of the coding strand of the F9 gene (exon 2-exon4) that you found in the online database (NCBI, Genomic sequence F9 gene). Some of the sequence is missing, but you know how many nucleotides are skipped. Design forward and reverse primers 18nt each for PCR that will isolate EXACTLY the sequence that is in bold typeface. Exon 2 = 164 nt Intron 2...
S-CGT-3 GTG 3. Write in the following sequences to depict them hybridizing/annealing to complementary and antiparallel sequence in the exposed nucleotide chains. 5'-GTG-3 5-CGT-3 5'-ACG-3' | 5 -CAC-3" 5'-AAT[CGTATCAGCAGCAGTG|ACT-3 -3'-TTALGCATAGTCGTCGTCATGA-5'- 3. Two of the four above sequences can be used together as a "primer pair" to PCR amplify the bracketed sequence. In order to determine which two will work, recall that new polynucleotide chains can only be added to on the 3'end. Draw an arrow from the 3' end of...
2. Design a primer pair (5 nucleotides each) to amplify only exon 2 of the gene model below. Use the primer pair you designed to model 3 cycles of DNA replication. 5..CGTGACTGATCATGGAGCTACGATCGACTCTCGAAGCTCTAGACGCTCTCGAGAGCTCTCGAATTAGATCGTAATTGCGGTTCA...3'
The Psilotum nudum genome is 2.5 x 1011 bp. You wish to design a primer to amplify a specific gene in the genome. - Part A In general, what length of oligonucleotide would be sufficient to amplify a single unique sequence? To simplify your calculation, assume that all bases occur with an equal frequency. Hint: The frequency of occurrence of a PCR primer in genomic DNA is given by this equation: genomic DNA length x sequence frequency The sequency frequency...
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