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You want to amplify the DNA between the two stretches of sequence shown below. Of the...

You want to amplify the DNA between the two stretches of sequence shown below. Of the listed primer pairs, choose the correct pair that will allow you to amplify the DNA by PCR.

5’-CTGGACGAAA----TGTAATGGTT-3’

3’-GACCTGCTTT----ACATTACCAA-5’

A. 5’-CTGGACGAAA-3’ and 5’-TGTAATGGTT-3’

B. 5’-CTGGACGAAA-3’ and 5’-AACCATTACA-3’

C. 5’-TTTCGTCCAG-3’ and 5’-TGTAATGGTT-3’

D. 5’-GACCTGCTTT-3’ and 5’-ACATTACCAA-3’

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Answer #1

Option b

5' CTGGACGCAA..... TGTAATGGTT3'

3'GACCTGCTTTT......ACATTACCAA5'

DENATURATION AND ANNEALING

5'CTGGACGAAA......TGTAATGGTT3'

<-ACATTACCAA5'

5'CTGGACGGAAA->

3'GACCTGCTTTT...... ACATTACCAA5'

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