Question

You want to use PCR to amplify the entire DNA shown in the figure below. Of the listed primers, choose the pair that will allow you to amplify this stretch of DNA by PCR. (9 points total)
DNA to be amplified
5’-CGATTCGAGCCATTCGAACTCGATCAGCCGATTCGATCAACCTTGGACAGTCAG-3’
3’-GCTAAGCTCGGTAAGCTTGAGCTAGTCGGCTAAGCTAGTTGGAACCTGTCAGTC-5’
Primers:
(1) 5’-GCTAAGCTCGGTA-3’ (5) 5’-CTTGGACAGTCAG-3’
(2) 5’-GAACCTGTCAGTC-3’ (6) 5’-CGATTCGAGCCAT-3’
(3) 5’-CTGACTGTCCAAG-3’ (7) 5’-ATGGCTCGAATCG-3’
(4) 5’-GACTGACAGGTTC-3’ (8) 5’-TACCGAGCTTAGC-3’
Answer: I will need primer number _____ and primer number _____

Answer 1

Answer. For identify primer of a gene we must look for complementary sequence. There are two types of primer 1 forward primer and 2 reverse primer.

For forward primer the base are complementary to sequence from 5' side

And for reverse primer the complementary side will of 3' side from last

In this case option 1 and 5 are correct which make complementary for both cases (forward and reverse primer)

(1) 5’-GCTAAGCTCGGTA-3’

(5) 5’-CTTGGACAGTCAG-3’