Homework Answers
Decrease
If the concentration of substrate is decreased, then the rate of reaction will also decrease. This is because less number of substrate molecules will be available for binding to the enzyme molecules. Hence less number of enzyme substrate complexes will be formed and therefore less number of product molecules will be formed. Overall the speed of the reaction will decrease.
So, the slope of the graph will become more negative in comparison to what it is shown in the image right now and the slope will decrease.
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Experiment 1 Experiment 2 Rate of reaction Rate of reaction Increasing concentration does not affect reaction...
Experiment 1 Experiment 2 Rate of reaction Rate of reaction Increasing concentration does not affect reaction...
Experiment 1 Experiment 2 Rate of reaction Rate of reaction Increasing concentration does not affect reaction rate Enzyme concentration Substrate Concentration What has become rate limiting in Experiment 2 at (and after) the green X?
2. We can measure how fast the Product is formed at increasing levels of Substrate Concentration...
2. We can measure how fast the Product is formed at increasing levels of Substrate Concentration The rate of the reaction can be plotted below. This is called a Michaelis-Menten Graph and it shows several important features of the enzyme: a. What happens to the reaction rate as the amount of substrate is increased? reaction rate- b. Why does the graph level off at high levels of substrate concentration? substrate concentration
2. Why does increasing the amount of substrate increase the amount of color produced in the...
2. Why does increasing the amount of substrate increase the
amount of color produced in the test tubes?
ght questions: etermina un in the 1. In Activity 1, what is the purpose of the test tube containing only w test tube containing only water and substrate? 2. Why does increasing the amount of substrate increase the amount of con strate increase the amount of color produced in the test tubes? 3. If you were to look at the three test...
1. a) If one were to decrease the enzyme concentration by half, how would this affect...
1. a) If one were to decrease the enzyme concentration by half, how would this affect the Vmaxvalue? Explain why this is so. What assumption are we making in regards to the relative amounts of substrate to enzyme? b) Is the turnover rate (kcat value) of an enzyme expected to change if the enzyme concentration was decreased by half? Briefly explain. c) Is the turnover rate (kcat value) of an enzyme expected to change in the presence of a competitive...
The concentration of substrate X is low. What happens to the rate of the enzyme- catalyzed reaction if the concentr...
The concentration of substrate X is low. What happens to the rate of the enzyme- catalyzed reaction if the concentration of X is doubled? 15. What effect does an increase in the enzyme concentration have on the rate of an enzyme-catalyzed reaction? 16.
max Rate constan 20- 10 A 85 X tot Temperature props When was the reaction rate...
max Rate constan 20- 10 A 85 X tot Temperature props When was the reaction rate lowest? QUESTIONS 1. What is the optimum temperature? Explain. 2. How does changing the temperature affect the rate of enzyme activity? Does this follow a pattern you anticipated? 3. Why might the enzyme activity decrease at very high temperatures? Testing the Effect of Substrate Concentration 65
8. Know how increasing the initiator concentration does affect the rate of polymers formed by cationic...
8. Know how increasing the initiator concentration does affect the rate of polymers formed by cationic polymerization.
what do you think will happen to the rate of reaction if one increases the concentration...
what do you think will happen to the rate of reaction if one increases the concentration of substrate to 6ml? why does the speed of reaction level off?
2-In an enzyme-catalyzed reaction, the rate of the reaction depends on which of the following at...
2-In an enzyme-catalyzed reaction, the rate of the reaction depends on which of the following at very low substrate concentrations? Select one: Neither enzyme concentration nor substrate concentration Enzyme concentration but not substrate concentration Substrate concentration but not enzyme concentration Both substrate concentration and enzyme concentration
The key factor that controls the initial rate of an enzyme catalysed reaction (Vo) is the...
The key factor that controls the initial rate of an enzyme catalysed reaction (Vo) is the concentration of the substrate of the reaction ([S]). In Damon's Michaelis-Menten experiment, the highest concentration of substrate used was 500 UM. What do you think will happen to the reaction velocity if higher concentrations of substrate were used? Select one: a. Vo will reach a plateau at higher (S) values O b. Vo will increase exponentially as (S) is increased O c. Vo will...
Experiment 1 Experiment 2 Rate of reaction Rate of reaction Increasing concentration does not affect reaction rate Enzyme concentration Substrate Concentration What has become rate limiting in Experiment 2 at (and after) the green X?
2. We can measure how fast the Product is formed at increasing levels of Substrate Concentration The rate of the reaction can be plotted below. This is called a Michaelis-Menten Graph and it shows several important features of the enzyme: a. What happens to the reaction rate as the amount of substrate is increased? reaction rate- b. Why does the graph level off at high levels of substrate concentration? substrate concentration
2. Why does increasing the amount of substrate increase the
amount of color produced in the test tubes?
ght questions: etermina un in the 1. In Activity 1, what is the purpose of the test tube containing only w test tube containing only water and substrate? 2. Why does increasing the amount of substrate increase the amount of con strate increase the amount of color produced in the test tubes? 3. If you were to look at the three test...
The concentration of substrate X is low. What happens to the rate of the enzyme- catalyzed reaction if the concentration of X is doubled? 15. What effect does an increase in the enzyme concentration have on the rate of an enzyme-catalyzed reaction? 16.
max Rate constan 20- 10 A 85 X tot Temperature props When was the reaction rate lowest? QUESTIONS 1. What is the optimum temperature? Explain. 2. How does changing the temperature affect the rate of enzyme activity? Does this follow a pattern you anticipated? 3. Why might the enzyme activity decrease at very high temperatures? Testing the Effect of Substrate Concentration 65
8. Know how increasing the initiator concentration does affect the rate of polymers formed by cationic polymerization.
The key factor that controls the initial rate of an enzyme catalysed reaction (Vo) is the concentration of the substrate of the reaction ([S]). In Damon's Michaelis-Menten experiment, the highest concentration of substrate used was 500 UM. What do you think will happen to the reaction velocity if higher concentrations of substrate were used? Select one: a. Vo will reach a plateau at higher (S) values O b. Vo will increase exponentially as (S) is increased O c. Vo will...
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