2. Why does increasing the amount of substrate increase the amount of color produced in the test tubes?
Homework Answers
2. Increase in the amount of substrate concentration increases the rate of reaction ,there by more substrate react with enzymes and more products are formed .hence the amount of colour produced are increased in the test tubes.
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2. Why does increasing the amount of substrate increase the
amount of color produced in the...
4. Both high and low temperatures reduced the amount of color (product) produced. However, the cause...
4. Both high and low temperatures reduced the amount of color
(product) produced. However, the cause of the reduction is
different in the two cases. Explain what the differences are.
me. The wa Activity 2: temperature In this activity, you will examine the effect of different temperatures on the activity of the enzyme. and enzyme mixture must come to a specific tempera yme mixture must come to a specific temperature before the addition of the substrate. 1. Write your hypothesis...
Amount of Substrate (9) Test Tube Number 1 2 3 4 5 1.0 1.0 1.0 1.0...
Amount of Substrate (9) Test Tube Number 1 2 3 4 5 1.0 1.0 1.0 1.0 1.0 Number of Molecules of Product Formed per Minute (x10%) 145 145 145 145 145 2 3 4 pH7 pH7 pH7 PH pH7 37°C 0.5 g substrate 1.0 9 substrate 2.0 g substrate 4.0 9 substrate 8.0 g substrate 4 Information Reset A 2 MacBook Air QUESTION 3 Set the test tubes to pH 3, 5, 7, 9, and 11 and use 1.0 g...
What causes the lack of on in tube # 5 that is acting as the control...
What causes the lack of on in tube # 5 that is acting as the control tube? 13. What is the reason for using the control tube What is the reason for 5. used in this exercise 14. What is the relationship between the concentration of pepsin and enzyme activity, as observed on Figure 3-7? 15. How does an increase in the amount of the pepsin, in the test tube, enhance the interaction between pepsin and substrate, thus speeding up...
3. Why was the concentration of catechol in test tube 2 and 4? Caps Lo 448...
3. Why was the concentration of catechol in test tube 2 and
4?
Caps Lo 448 1. I You will determine 2. Why Shift 1 he sequence given in the Ctrl record whether or Activity 6 - Enzyme Inhibition This activity will investigate the inhibition of catechol oxidase by phenylthiourea (PTU). You w PTU is a competitive or noncompetitive inhibitor. 1. Write your hypothesis and prediction. 2. Obtain a bottle of PTU. 3. Using Table 6.1, prepare three experimental tubes....
values differ, give reasons as why they are different. ruct Table 2. Results of the Biuret...
values differ, give reasons as why they are different. ruct Table 2. Results of the Biuret Test Color with Biuret Reagent Test Tube Contents Questions: 1) Which tubes contain protein? Describe a positive biuret test for protein based on your experimental results. the Rpomin Cole tor 丹 ts in proteins 2) Based on your qualitative (visual) observations of the Biuret test results, do all three tubes contain the same amount of protein? How do you know? 3) Explain method by...
Procedure: 1. 10 Test tubes were labelled (0C,0E,15C,15E,30C,30E,45C,45E,60C, and 60E.) 2. 2 mL cathecol-buffer at pH...
Procedure:
1. 10 Test tubes were labelled
(0C,0E,15C,15E,30C,30E,45C,45E,60C, and 60E.)
2. 2 mL cathecol-buffer at pH 6 was added into each test
tubes.
3. 3.5 mL of water was added into all of the C tubes
(0C,15C,30C,45C, and 60C.)
4. 3 mL of water was added into the remaining tubes.
5.The two tubes were put into an ice bath.
6. 0.5 mL of enzyme polyphenoloxidase was added into tube E when
the mixture had reached certain T (O°C)
7.The transmittance...
Questions 1 and 2 Ionescent molten lava. Living LIVE II only a limited portion of the...
Questions 1 and 2
Ionescent molten lava. Living LIVE II only a limited portion of the Earth's temperature range. In fact, the activities of life are possible only in the range from around 0°C (the freezing point of water) to 100°C (the boiling point of water). Of course, there are always exceptions to this rule. In some instances, life can survive at temperatures well below freezing and some organisms are adapted to very high temperatures. PROCEDURE (GENERAL EXPERIMENTAL SET-UP) Just...
Part A Explain why glucose consumption must increase in hypoxic tissues to provide the same amount...
Part A Explain why glucose consumption must increase in hypoxic tissues to provide the same amount of ATP that could be produced from glucose in normoxic (normal O, levels) tissues. et the eprivation mple, in for over reased the tect Jucible factor glycolytic alytic e starved reasing Bases. In hypox tissues, in contrast to normoxic tissues, ATP is additionally consumed to transform lactate to pyruvate Additional glucose is consumed by hipoxic tissues to be metabolized through the glyoxylate pathway In...
For each experiment described below 1) provide the relevant equilibrium reaction or reactions, 2) use LeChatelier's...
For each experiment described below 1) provide the relevant equilibrium reaction or reactions, 2) use LeChatelier's Principle to explain why the reaction has shifted left (L) or right (R), and 3) answer any other questions. a) To a solution containing 1M Pb(NO3)2, 1 M HCl is slowly added until a white precipitate forms. The mixture is then heated and the precipitate disappears. Is the dissociation of the precipitate endothermic or exothermic? Does the equilibrium constant (Ksp) change when the solution...
Foundations of Biological Sciences 1 Enzymes - 8 PART 4: THE EFFECT OF PH ON ENZYME...
Foundations of Biological Sciences 1 Enzymes - 8 PART 4: THE EFFECT OF PH ON ENZYME ACTIVITY The pll scale measures the amount Another factor influencing the rate of enzyme catalysis is the pll of the of ons in a solution. The more solution. pH specifies the acidity or basicity of solution by measuring the the more acidic the solution and lower hydrogen ion consentation (H). Like temperature, pH affects the three- pH) dimensional shape of enzymes, thus regulating their...
4. Both high and low temperatures reduced the amount of color
(product) produced. However, the cause of the reduction is
different in the two cases. Explain what the differences are.
me. The wa Activity 2: temperature In this activity, you will examine the effect of different temperatures on the activity of the enzyme. and enzyme mixture must come to a specific tempera yme mixture must come to a specific temperature before the addition of the substrate. 1. Write your hypothesis...
Amount of Substrate (9) Test Tube Number 1 2 3 4 5 1.0 1.0 1.0 1.0 1.0 Number of Molecules of Product Formed per Minute (x10%) 145 145 145 145 145 2 3 4 pH7 pH7 pH7 PH pH7 37°C 0.5 g substrate 1.0 9 substrate 2.0 g substrate 4.0 9 substrate 8.0 g substrate 4 Information Reset A 2 MacBook Air QUESTION 3 Set the test tubes to pH 3, 5, 7, 9, and 11 and use 1.0 g...
What causes the lack of on in tube # 5 that is acting as the control tube? 13. What is the reason for using the control tube What is the reason for 5. used in this exercise 14. What is the relationship between the concentration of pepsin and enzyme activity, as observed on Figure 3-7? 15. How does an increase in the amount of the pepsin, in the test tube, enhance the interaction between pepsin and substrate, thus speeding up...
3. Why was the concentration of catechol in test tube 2 and
4?
Caps Lo 448 1. I You will determine 2. Why Shift 1 he sequence given in the Ctrl record whether or Activity 6 - Enzyme Inhibition This activity will investigate the inhibition of catechol oxidase by phenylthiourea (PTU). You w PTU is a competitive or noncompetitive inhibitor. 1. Write your hypothesis and prediction. 2. Obtain a bottle of PTU. 3. Using Table 6.1, prepare three experimental tubes....
values differ, give reasons as why they are different. ruct Table 2. Results of the Biuret Test Color with Biuret Reagent Test Tube Contents Questions: 1) Which tubes contain protein? Describe a positive biuret test for protein based on your experimental results. the Rpomin Cole tor 丹 ts in proteins 2) Based on your qualitative (visual) observations of the Biuret test results, do all three tubes contain the same amount of protein? How do you know? 3) Explain method by...
Procedure:
1. 10 Test tubes were labelled
(0C,0E,15C,15E,30C,30E,45C,45E,60C, and 60E.)
2. 2 mL cathecol-buffer at pH 6 was added into each test
tubes.
3. 3.5 mL of water was added into all of the C tubes
(0C,15C,30C,45C, and 60C.)
4. 3 mL of water was added into the remaining tubes.
5.The two tubes were put into an ice bath.
6. 0.5 mL of enzyme polyphenoloxidase was added into tube E when
the mixture had reached certain T (O°C)
7.The transmittance...
Questions 1 and 2
Ionescent molten lava. Living LIVE II only a limited portion of the Earth's temperature range. In fact, the activities of life are possible only in the range from around 0°C (the freezing point of water) to 100°C (the boiling point of water). Of course, there are always exceptions to this rule. In some instances, life can survive at temperatures well below freezing and some organisms are adapted to very high temperatures. PROCEDURE (GENERAL EXPERIMENTAL SET-UP) Just...
Part A Explain why glucose consumption must increase in hypoxic tissues to provide the same amount of ATP that could be produced from glucose in normoxic (normal O, levels) tissues. et the eprivation mple, in for over reased the tect Jucible factor glycolytic alytic e starved reasing Bases. In hypox tissues, in contrast to normoxic tissues, ATP is additionally consumed to transform lactate to pyruvate Additional glucose is consumed by hipoxic tissues to be metabolized through the glyoxylate pathway In...
Foundations of Biological Sciences 1 Enzymes - 8 PART 4: THE EFFECT OF PH ON ENZYME ACTIVITY The pll scale measures the amount Another factor influencing the rate of enzyme catalysis is the pll of the of ons in a solution. The more solution. pH specifies the acidity or basicity of solution by measuring the the more acidic the solution and lower hydrogen ion consentation (H). Like temperature, pH affects the three- pH) dimensional shape of enzymes, thus regulating their...
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