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Amount of Substrate (9) Test Tube Number 1 2 3 4 5 1.0 1.0 1.0 1.0...
2. Why does increasing the amount of substrate increase the amount of color produced in the...
2. Why does increasing the amount of substrate increase the
amount of color produced in the test tubes?
ght questions: etermina un in the 1. In Activity 1, what is the purpose of the test tube containing only w test tube containing only water and substrate? 2. Why does increasing the amount of substrate increase the amount of con strate increase the amount of color produced in the test tubes? 3. If you were to look at the three test...
What causes the lack of on in tube # 5 that is acting as the control...
What causes the lack of on in tube # 5 that is acting as the control tube? 13. What is the reason for using the control tube What is the reason for 5. used in this exercise 14. What is the relationship between the concentration of pepsin and enzyme activity, as observed on Figure 3-7? 15. How does an increase in the amount of the pepsin, in the test tube, enhance the interaction between pepsin and substrate, thus speeding up...
4. Both high and low temperatures reduced the amount of color (product) produced. However, the cause...
4. Both high and low temperatures reduced the amount of color
(product) produced. However, the cause of the reduction is
different in the two cases. Explain what the differences are.
me. The wa Activity 2: temperature In this activity, you will examine the effect of different temperatures on the activity of the enzyme. and enzyme mixture must come to a specific tempera yme mixture must come to a specific temperature before the addition of the substrate. 1. Write your hypothesis...
please show how to calculate the substrate concentration in the test tubes at time=zero point 3...
please show how to calculate the substrate concentration in
the test tubes at time=zero point
3 .375 0.84-0.bda 2min What is the amount of enzyme that you chose to use in Part B? Part B- Dis cusion 1. Calculate the units of enzyme activity for the varying amounts of sub- strate. Calculate the substrate concentrations in the test tubes at time = zero point. Substrate Volume (mL) L-DOPA (mM) AA479/min umol/min 0.05 0.jas 0. 10 4 0. a5 0.1 0.208...
1 2 3 4 5 6 7 8 9 Part A Which of the following is/are...
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Part A Which of the following is/are means whereby a catalyst can lower the activation energy of a reaction? quantum tunneling decreasing the number of reactive molecules permanently binding substrates inefficient collisions altering the temperature within the cell to one appropriate for reactions to proceed Subrnit Request Answer Part A An enzyme Obinds substrates in a manner that facilitates the formation of product decreases the rate of a reaction. is always...
help 5 and 6 Question 5 --/2 In this exercise, we are going to examine the...
help 5 and 6
Question 5 --/2 In this exercise, we are going to examine the effect of the following factors that affect the activity of enzymes: (Select all that apply) ability of the enzyme in catalyzing different potential substrates concentration of substrate 3 temperature 4 pH (5) need of copper for this enzyme activity What is PTU (phenylthiourea) and why we will be using it in exercise 5.6 (1) PTU is a cofactor of catechol oxidase PTU will remove...
Sucrose Levels Data: Tube # 1 2 3 4 5 6 7 8 9 `0 11...
Sucrose Levels Data: Tube # 1 2 3 4 5 6 7 8 9 `0 11 Water (mL) 1.0 0.75 0.5 0.25 Glucose Std. (mL) 0.25 0.5 0.75 1.0 Sucrose Std. (mL) 1.0 1.0 CONC. Extract (mL) 1.0 1.0 Diluted Extract (mL) 1.0 1.0 Buffer (mL) 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 TUBE VS ABSORBANCES: 1 blank 2 .071 3 .535 4 .551 5 .042 6 0 7 .306 8 .056 9 1.999 10 .025 11 .35 A)...
C. EFFECT OF pH Starch test Color Activity pH-2 Dark brown pH 4 p darlc brou) H 10 Graphing of ...
chart C
graph
gor part C
C. EFFECT OF pH Starch test Color Activity pH-2 Dark brown pH 4 p darlc brou) H 10 Graphing of Enzymatic Activity (Enzyme activity vs. pH) High Enzyme Activity Level Low 10 4. pH D. EFFECT OF INHIBITION Starch test 95% Ethanol Color DI Water AgNO color 1 Activity | Colorano Dark Activity Activity Activity Color Tign+ Crold bive Glucose test TBenediet's) 95% Ethanol Color AgNO lor一一. Activity DI Water Color Activity Activity 7....
Foundations of Biological Sciences 1 Enzymes - 8 PART 4: THE EFFECT OF PH ON ENZYME...
Foundations of Biological Sciences 1 Enzymes - 8 PART 4: THE EFFECT OF PH ON ENZYME ACTIVITY The pll scale measures the amount Another factor influencing the rate of enzyme catalysis is the pll of the of ons in a solution. The more solution. pH specifies the acidity or basicity of solution by measuring the the more acidic the solution and lower hydrogen ion consentation (H). Like temperature, pH affects the three- pH) dimensional shape of enzymes, thus regulating their...
Standard Curve Test Tube Absorbance [Fe(SCN)2+] 1 0.073 1.0*10-5 2 0.18 2.0*10-5 3 0.212 3.0*10-5 4...
Standard Curve Test Tube Absorbance [Fe(SCN)2+] 1 0.073 1.0*10-5 2 0.18 2.0*10-5 3 0.212 3.0*10-5 4 0.298 4.0*10-5 5 0.353 5.0*10-5 Finish the table by taking the absorbance of each solution and read the molarity from your standard curve. Test Tube Absorbance [Fe(SCN)2+] 6 0.132 7 0.238 8 0.341 Please show work!! Thank you!!
2. Why does increasing the amount of substrate increase the
amount of color produced in the test tubes?
ght questions: etermina un in the 1. In Activity 1, what is the purpose of the test tube containing only w test tube containing only water and substrate? 2. Why does increasing the amount of substrate increase the amount of con strate increase the amount of color produced in the test tubes? 3. If you were to look at the three test...
What causes the lack of on in tube # 5 that is acting as the control tube? 13. What is the reason for using the control tube What is the reason for 5. used in this exercise 14. What is the relationship between the concentration of pepsin and enzyme activity, as observed on Figure 3-7? 15. How does an increase in the amount of the pepsin, in the test tube, enhance the interaction between pepsin and substrate, thus speeding up...
4. Both high and low temperatures reduced the amount of color
(product) produced. However, the cause of the reduction is
different in the two cases. Explain what the differences are.
me. The wa Activity 2: temperature In this activity, you will examine the effect of different temperatures on the activity of the enzyme. and enzyme mixture must come to a specific tempera yme mixture must come to a specific temperature before the addition of the substrate. 1. Write your hypothesis...
please show how to calculate the substrate concentration in
the test tubes at time=zero point
3 .375 0.84-0.bda 2min What is the amount of enzyme that you chose to use in Part B? Part B- Dis cusion 1. Calculate the units of enzyme activity for the varying amounts of sub- strate. Calculate the substrate concentrations in the test tubes at time = zero point. Substrate Volume (mL) L-DOPA (mM) AA479/min umol/min 0.05 0.jas 0. 10 4 0. a5 0.1 0.208...
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Part A Which of the following is/are means whereby a catalyst can lower the activation energy of a reaction? quantum tunneling decreasing the number of reactive molecules permanently binding substrates inefficient collisions altering the temperature within the cell to one appropriate for reactions to proceed Subrnit Request Answer Part A An enzyme Obinds substrates in a manner that facilitates the formation of product decreases the rate of a reaction. is always...
help 5 and 6
Question 5 --/2 In this exercise, we are going to examine the effect of the following factors that affect the activity of enzymes: (Select all that apply) ability of the enzyme in catalyzing different potential substrates concentration of substrate 3 temperature 4 pH (5) need of copper for this enzyme activity What is PTU (phenylthiourea) and why we will be using it in exercise 5.6 (1) PTU is a cofactor of catechol oxidase PTU will remove...
chart C
graph
gor part C
C. EFFECT OF pH Starch test Color Activity pH-2 Dark brown pH 4 p darlc brou) H 10 Graphing of Enzymatic Activity (Enzyme activity vs. pH) High Enzyme Activity Level Low 10 4. pH D. EFFECT OF INHIBITION Starch test 95% Ethanol Color DI Water AgNO color 1 Activity | Colorano Dark Activity Activity Activity Color Tign+ Crold bive Glucose test TBenediet's) 95% Ethanol Color AgNO lor一一. Activity DI Water Color Activity Activity 7....
Foundations of Biological Sciences 1 Enzymes - 8 PART 4: THE EFFECT OF PH ON ENZYME ACTIVITY The pll scale measures the amount Another factor influencing the rate of enzyme catalysis is the pll of the of ons in a solution. The more solution. pH specifies the acidity or basicity of solution by measuring the the more acidic the solution and lower hydrogen ion consentation (H). Like temperature, pH affects the three- pH) dimensional shape of enzymes, thus regulating their...
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