Question

me. The wa Activity 2: temperature In this activity, you will examine the effect of different temperatures on the activity of the enzyme. and enzyme mixture must come to a specific tempera yme mixture must come to a specific temperature before the addition of the substrate. 1. Write your hypothesis and prediction 2. Label five test tubes 1-5. Add 10mL of distilled water and L of distilled water and 10 drops of enzyme to each tube. Place tube 1 in an ice bath for 5 minutes. Similarly, place tubes 2-5 in water baths of 20°, 40°, 60° and 100°C, respectively, for 5 minutes. 4. After allowing the tubes to come to their app ing the tubes to come to their appropriate temperature, add 10 drops of substrate to each tube. Cap the tubes and mix. Loosen the caps and immediately and mix. Loosen the caps and immediately return the tubes to their appropriate water baths for an additional 5 minutes. 5. After 5 minutes, remove the test tubes from the way es, remove the test tubes from the water baths and observe the color of the tubes. Record or intensity of each tube in Table 2.2 and rank them from darkest to lightest. Table 2.1 - Results of Activity 2 #2 tl Tube Temperature (°C) Color after 5 Rank (+, ++, +++) minutes 1 0° light yellow-t ellow 20° brown-yellow Imed. Yellow- tt 40° dark brown dark brown-ttt ANPINK Pinta o ter 900 Light Pink light pinho ++ 60° ttt 20 100 60 60 80 20100 120

4. Both high and low temperatures reduced the amount of color (product) produced. However, the cause of the reduction is different in the two cases. Explain what the differences are.

Answer 1

Enzymes are most active at an optimum temperature. Decreasing or increasing temperatu re to extreme values leads to shaly decrease in activity. In this experiment when you incubated the enzyme to very low temperature like 0°c) you noticed very less activity. The reason is that at very low temperature the movement of molecules is slowed down. Due to reduced movement the frequency of collisions between enzyme and substrale is lowered hence the enzyme substrate 1 complex formation is tough. So you can say that reduced molecular movement and subsequent reduction name substrate collisions leads to very less activity at low temperature when you I increase temperature enzyme and substrate movement speeds up due to high Kinetic energy and enzyme substrade collisions increase hence you see increase in enzyme activity at optimum temperature. When temperature is too high there is denateration of enzyme. The denatured enzyme means it's shape is changed as a result of high temperature. Due to change in enzyme shape and disruption of shape of its active the enzyme shows reduction in activity, You have soccifically mentioned this question Camshoker you need this questions answer only. Scanned with C