Substrate concentration has become rate limiting in Experiment 2(and after) the green X.
The graph represents enzyme kinetics graph. The plateau of the graph occurs after a certain period of time because all the available enzyme molecule are saturated with the available substrate molecule and attained Vmax. After that point, even if more substrate molecules are added, no further increase of reaction rate will occur as no free enzymes are available. Hence, substrate concentration turns out to be the limiting factor.
Experiment 1 Experiment 2 Rate of reaction Rate of reaction Increasing concentration does not affect reaction...
Experiment 1 Experiment 2 Rate of reaction Rate of reaction Increasing concentration does not affect reaction rate Enzyme concentration Substrate Concentration In Experiment 1, what would happen to the slope of the line if you decreased the amount of substrate?
6 The graph shows the effect of substrate concentration on the rate of an enzyme- controlled reaction. The enzyme concentration is constant. reaction rate Which statement about the graph is correct? A Between Wand X, the substrate concentration number of enzyme molecules is limiting. Between X and Y, the number of enzyme molecules is limiting. Between X and Y, the number of substrate molecules is limiting. Between X and Y, the product concentration remains the same. Thanh in the nammanni...
2. We can measure how fast the Product is formed at increasing levels of Substrate Concentration The rate of the reaction can be plotted below. This is called a Michaelis-Menten Graph and it shows several important features of the enzyme: a. What happens to the reaction rate as the amount of substrate is increased? reaction rate- b. Why does the graph level off at high levels of substrate concentration? substrate concentration
The concentration of substrate X is low. What happens to the rate of the enzyme- catalyzed reaction if the concentration of X is doubled? 15. What effect does an increase in the enzyme concentration have on the rate of an enzyme-catalyzed reaction? 16.
The rate of an enzyme-catalyzed reaction initially increases with an increase in the substrate concentration, but eventually reaches a maximum value, even though the concentration of substrate continues to increase. Which of the following best explains why? O As substrate concentration increases, the substrates preferentially bind with each other instead of the active site of the enzyme, and no additional catalysis occurs. As substrate concentration increases, the active sites of all the enzyme molecules become occupied with substrate molecules, and...
2. Why does increasing the amount of substrate increase the amount of color produced in the test tubes? ght questions: etermina un in the 1. In Activity 1, what is the purpose of the test tube containing only w test tube containing only water and substrate? 2. Why does increasing the amount of substrate increase the amount of con strate increase the amount of color produced in the test tubes? 3. If you were to look at the three test...
max Rate constan 20- 10 A 85 X tot Temperature props When was the reaction rate lowest? QUESTIONS 1. What is the optimum temperature? Explain. 2. How does changing the temperature affect the rate of enzyme activity? Does this follow a pattern you anticipated? 3. Why might the enzyme activity decrease at very high temperatures? Testing the Effect of Substrate Concentration 65
8. Know how increasing the initiator concentration does affect the rate of polymers formed by cationic polymerization.
2-In an enzyme-catalyzed reaction, the rate of the reaction depends on which of the following at very low substrate concentrations? Select one: Neither enzyme concentration nor substrate concentration Enzyme concentration but not substrate concentration Substrate concentration but not enzyme concentration Both substrate concentration and enzyme concentration
The graph below shows an enzyme- controlled reaction. The enzyme concentration is kept constant. At concentrations of substrate greater than X, which of the following statements is true? Rate of reaction 0 Concentration of substrate X The rate of reaction is limited by enzyme concentration. The rate of reaction tends towards zero. The substrate has an inhibitory effect. The products have an inhibitory effect.