Question

Confused on this problem as i'm getting mixed answers regarding this question.

Is option 1 false because immortalisation requires whole episomal form of EBV and 9 proteins and here we are transfecting with only one protein 2? However, I read in an article that "Lymphoblastoid cell lines (LCLs) are human B cells latently infected and immortalized by Epstein-Barr virus (EBV). A mini-EBV plasmid that consists of less than half the EBV genome can still immortalize B cells in vitro." which makes me think option 1 is true? Since Dr. Immuno is changing a virus encoding protein and for this to happen i'm assuming he would do this in vitro??? Option 2 is false since BCR signaling will increase, So i think E is eliminated.

I think it is between C and D...

Epstein-Barr virus (EBV) is a common human pathogen that is associated with a variety of hemopoietic cancers such as a African Burkitt's lymphoma, Hodgkin's disease, adult T-cell leukemia, and lymphoproliferative diseases. B lymphocytes infected with EBV and grown in vitro are immortalized and are termed lymphoblastoid cell lines (LCLs). These EBV-transformed LCLS contain EBV episomes and nine virus-encoded proteins. One of them is LMP2A. The LMP2A protein localizes to numerous small patches in the plasma membrane of the infected B lymphocytes. Within the 119-amino-acid amino-terminal domain of LMP2A there are 8 tyrosine residues. 2 of which form an immunoreceptor tyrosine-based activation motif (ITAM). Antigen-induced B-lymphocyte proliferation and differentiation result from the sequential activation of protein tyrosine kinases and their interaction with phosphorylated ITAMs. The 119- amino-acid cytoplasmic amino-terminal domain of LMP2A is essential for the pathogenic effect of EBV. With LMP2A of EBV, Dr. Immunob has recently generated a plasmid construct encoding "Always- Y-phospho-LMP2A" protein, which is a modified form of LMP2A. "Always-Y-phospho-LMP2A" can not only be constitutively clustered at the plasma membrane, but also maintain the tyrosine phosphorylated status at the ITAM motif of LMP2A. When B-cells are transfected with this expression construct of "Always-Y-phospho-LMP2A", what consequence(s) can you expect to observe? Find a correct combination from the following indications. 1. The transfected B cells will become immortalized. II. B-cell receptor (BCR)-induced signaling will be reduced in these B cells. III. Increased PIP3 production will be observed in these B cells. IV. Increased NFKB activation will be observed in these B cells.

Answer 1

(D​​​​​​). I and III and IV is the correct option. That is

I. The tramsfected B cells will become immortalized.

III. Increased PIP3 production will be observed in these B cells.

IV. Increased NFKB activation will be observed in these B cells.