Homework Answers
Here, unidirectional clearly represent that replication occur through one end.
Correct option is : b) 3H thymidine , one end
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You are measuring DNA replication in E. coli cells using radioisotopes. If DNA replication was unidirectional...
Error rates in DNA replication, proofreading and mismatch repair systems in E. coli are about 10-10...
Error rates in DNA replication, proofreading and mismatch repair systems in E. coli are about 10-10 per base pair per round of replication. Based on this, how many mutations per base pair would you expect to see in a population of 1010 E. coli cells (e.g. of a TSB culture of E. coli)
You have isolated an E. coli mutant which is not able to complete DNA replication. Instead,...
You have isolated an E. coli mutant which is not able to complete DNA replication. Instead, only short fragments of DNA are made. Which enzyme would you suspect is mutated? A. Ligase B. Primase C. Helicase
Fill in the Blank 1. DNA replication in E. coli begins at a 245 base pair...
Fill in the Blank 1. DNA replication in E. coli begins at a 245 base pair sequence called • The initiation of DNA replication at this site begins with the binding of the protein, to multiple 9 base pair repeats regions. The association of these proteins generate superhelical tension which unwinds the 13 bp repeat region called the . The next step of initiation involves the loading of the protein (a helicase) onto the 13 bp repeat region by another...
Which aspect of the E. coli DNA replication machinery accounts for high processivity? a. The beta...
Which aspect of the E. coli DNA replication machinery accounts for high processivity? a. The beta sliding clamp of DNA Polymerase III b. 3’-5’ exonuclease activity of DNA Polymerase I c. 5’-3’ exonuclease activity of DNA Polymerase I d. Single strand DNA binding protein (SSB) e. The core subunit of DNA Polymerase III An explanation of the solution would be greatly appreciated, thanks!
To place the E. coli replication system on the human scale, imagine that the DNA strand...
To place the E. coli replication system on the human scale, imagine that the DNA strand type B has a diameter of 20 angstroms and that it expands to 1m in diameter. If this expansion happened for the holoenzyme DNA polymerase III, it would be about the size of almost half a truck. In this expanded system. (a) How fast each replisome would move. (b) How far would each replisome travel? (c) What size would an Okasaki fragment be? (d)...
You start with a cell that is grown in normal media. Subsequently DNA is allowed to...
You start with a cell that is grown in normal media. Subsequently DNA is allowed to replicate in an environment with all necessary enzymes, dATP, dCTP, dGTP, and radioactively labeled dTTP (3H thymidine) for 1 cell cycle, (resulting in 2 cells) and then switched back to normal, nonradioactive medium. Cell are allowed to replicate once more, so you end up with 4 cells. How many of these 4 cells with have radioactive dTTP incorporated in their DNA? Select one: a....
please help answering You start with a cell that is grown in normal media. Subsequently DNA...
please help answering You start with a cell that is grown in normal media. Subsequently DNA is allowed to replicate in an environment with all necessary enzymes, dATP, dCTP, dGTP, and radioactively labeled dTTP (3H thymidine) for 1 cell cycle, (resulting in 2 cells) and then switched back to normal, nonradioactive medium. Cell are allowed to replicate once more, so you end up with 4 cells. How many of these 4 cells with have radioactive dTTP incorporated in their DNA?...
if you answer all of then I'll be sure to give you a thumbs up. thanks...
if you answer all of then I'll be sure to give you a
thumbs up. thanks
3. The following image depicts the results of the experiment Meselson & Stahl did to determine the mechanism of DNA replication 14-N 15-N DNA DNA Control Semiconservative **Using similar drawings of the test tube after two rounds of replication, PREDICT what banding patterns Meselson and Stahl would have seen in their tubes if DNA replication occurred using a: a. Conservative mechanism b. Dispersive mechanism...
Question 14 1 points Save Answer DNA replication poses several challenges to the cell that must...
Question 14 1 points Save Answer DNA replication poses several challenges to the cell that must be resolved for replication to proceed. All of the following are cellular solutions to the challenges posed by DNA replication except... O A. Helicase unwinds to the DNA to allow the replication fork to form. O B. Primase synthesizes an RNA primer since DNA polymerase can only extend an existing polynucleotide chain. O C. Higher eukaryotes utilize multiple origins of replication to reduce the...
Bacterial cells, such as E. coli, are surrounded by a fluid plasma membrane and enclosed by...
Bacterial cells, such as E. coli, are surrounded by a fluid plasma membrane and enclosed by a rigid cell wall that protects the cell from damage. You will recall that there is no nuclear envelope in prokaryotic cells, but rather a “nucleoid region” with a single chromosome that is continuous (circular), not linear, as in eukaryotes. All of the genes required for basic survival and reproduction are found in the main chromosome. In addition to the main chromosomes, some DNA...
Fill in the Blank 1. DNA replication in E. coli begins at a 245 base pair sequence called • The initiation of DNA replication at this site begins with the binding of the protein, to multiple 9 base pair repeats regions. The association of these proteins generate superhelical tension which unwinds the 13 bp repeat region called the . The next step of initiation involves the loading of the protein (a helicase) onto the 13 bp repeat region by another...
if you answer all of then I'll be sure to give you a
thumbs up. thanks
3. The following image depicts the results of the experiment Meselson & Stahl did to determine the mechanism of DNA replication 14-N 15-N DNA DNA Control Semiconservative **Using similar drawings of the test tube after two rounds of replication, PREDICT what banding patterns Meselson and Stahl would have seen in their tubes if DNA replication occurred using a: a. Conservative mechanism b. Dispersive mechanism...
Question 14 1 points Save Answer DNA replication poses several challenges to the cell that must be resolved for replication to proceed. All of the following are cellular solutions to the challenges posed by DNA replication except... O A. Helicase unwinds to the DNA to allow the replication fork to form. O B. Primase synthesizes an RNA primer since DNA polymerase can only extend an existing polynucleotide chain. O C. Higher eukaryotes utilize multiple origins of replication to reduce the...
Bacterial cells, such as E. coli, are surrounded by a fluid plasma membrane and enclosed by a rigid cell wall that protects the cell from damage. You will recall that there is no nuclear envelope in prokaryotic cells, but rather a “nucleoid region” with a single chromosome that is continuous (circular), not linear, as in eukaryotes. All of the genes required for basic survival and reproduction are found in the main chromosome. In addition to the main chromosomes, some DNA...
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