You have isolated an E. coli mutant which is not able to complete DNA replication. Instead,...
You have isolated an E. coli mutant which is not able to complete DNA replication. Instead, only short fragments of DNA are made. Which enzyme would you suspect is mutated?
A. Ligase
B. Primase
C. Helicase
Homework Answers
Solution: Option A is correct
In this question we can observe that DNA strands are formed but they are not joined. Now as we know that DNA Ligase is an enzyme that helps in the joining of DNA strands together by catalysing the formation of a phosphodiester bond, so this E.coli must have mutated Ligase.
(Primase helps in initiation of DNA synthesis and Helicases unwinds the DNA so these two options are incorrect.)
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