Question 13: What is a CpG site? A CpG island? How might a CpG island in...
Question 13: What is a CpG site? A CpG island? How might a CpG island in a gene’s promoter region allow it to be silenced in certain cell types?
Homework Answers
CpG islands or CpG sites are the regions on DNA which are rich in CG dinucleotide repeats. They are mainly present upstream of promoter region. They are important because they protect the promoter and downstream sequences from undergoing methylation due to mutation. Methylation tends to add methyl group to specific nucleotides on DNA thus, silencing the gene. Presence of CpG islands protects the gene from being silenced.
If CpG sequence is mutated, than the chances that promoter region will get methylated will increase. This will lead to silencing of promoter and its downstream gene and will decrease the rate of gene expression.
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Question 13: What is a CpG site? A CpG island? How might a CpG
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Search 13 How will a mutation in the active site df an enzyme that reducing substrate binding affect enzyme parameters? OS (2 points) Reduce Vm Reduce Km Increase km Reduce Vm and km 14 Which amino acid residue sequence would mostly likely compose a transmembrane segment of a protein? (2 Points) VLVAVLESFLLLSL GRRHLGFEATFFQ GRKKRRORRRPQ NVFKGNTISOKIS 15 What would be observed if purified IgG was boiled and not reduced and then applied to SDS- PAGE (2 points)
Question 5 (5 pts): For what function has evolution favored the preservation of CpG dinucleotides at gene promoters? Question 6 (10 pts): What is meant by saying that regulatory activity at gene promoters alone cannot be responsible for a cell's overall program of cell-type differentiation? Question 7 (5 pts): Why is phosphorylation of certain serine residues on the C-terminal domain of RNA Pol II so important during gene transcription? Question 8 (5 pts): Why is alternative RNA splicing so important...
(the long island
estuary ecosystem)
5. How might you modify the Long Island estuary ecosystem to produce the following population effects? (a) decrease blowfish abundance; maintain fluke abundance (b) decrease tern abundance; maintain osprey abundance 6. What might happen to a given ecosystem if the top carnivore biomass decreased due to disease? Why 7, why is a more complex ecosystem (ie., greater number of pathways) probably more stable than a simple ecosystem? We were unable to transcribe this image
would you please help me with this question?
2. How does site specific recombination allow DNA to b What types of mobile elements can move around the genome? What enzymes catalyze the process? e exchanged between unrelated DNA sequences?
12. Why might a stylette be used when intubating a patient? 13. How does one know that the endotube is in the trachea following intubation? 14. Why are there two inner cannulas with a fenestrated trach tube? What implications are there for using a suction catheter? 15. What things can be done to prevent infection at the tracheostomy site? 16. List two precautions when using a speaking valve with a tracheostomy tube?
Clinical Investigation Questions 13. What might glycosuria and proteinuria indicate? Explain how these could be produced. 14. What did examination of this student's urine sediment indicate? How might this and the mild hematuria relate to the fact that the student is female?
Question 13 (10 marks) Scientists observed populations of sea turtles on two islands: Turtle Island (TI) and Lizard Island (LI). The first year of observation, they noted that 700 turtles lived on Turtle Island and 200 turtles lived on Lizard Island. Let x;, zyi denote the numbers of turtles (in hundreds) on Turtle Island and Lizard Island after i years. Write this information as - F the vector po = |Y0 The scientists returned the following year and made the...
13 14
Search 13 How will a mutation in the active site df an enzyme that reducing substrate binding affect enzyme parameters? OS (2 points) Reduce Vm Reduce Km Increase km Reduce Vm and km 14 Which amino acid residue sequence would mostly likely compose a transmembrane segment of a protein? (2 Points) VLVAVLESFLLLSL GRRHLGFEATFFQ GRKKRRORRRPQ NVFKGNTISOKIS 15 What would be observed if purified IgG was boiled and not reduced and then applied to SDS- PAGE (2 points)
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