Question

2) Eight different point mutations are studied by complementation test and by der mapping. The results of all pair-wise complementation tests between shown below. A) Classify these eight mutants into genes. 1 2 3 4 B) The eight point mutations are crossed with three deletion mutants. Taking these and the complementation data into account, indicate the order of mutations 1-8. Draw circles around paired mutations in the same gene. Also draw three lines beneath the map to indicate the position and extent of deletions I, II, III. 4 5 6 7 8

Answer 1

Answer:

Complementation occurs when two mutations are su different genes. + = Showing Complementation So, after reading this table, we can conclude that Mutations 8, 1, 2, 3, 5, 6, all are on diff genes but 884 are on ant gone Einrilarly, 7 & 1 are on one gene 642 - 543 So there are 4 genes on which there are 2 mutations each. deletion mapping. + indicates wildtype phenotype It becurs when deletion and hoint mutation denot coincide with each others 2B)

After considering both tables, we came to know that consider th tables, We Came to know that is having mut" del 1 del 2 del 3 - - - 1, 2, 6, 7 2,6,7,8 1, 2, 3, 7 and 844 741 642, 543 are on u diff genes. gene 1 gene 2 gene 3 gene 4 (5 3) 1 7 6 2 6 8 u the Order of mute, = is del 1 del 2 del 3