Question

B12030 (12) Defining the gene E cow mutants are isolated that cannot utilize tetrahydro-cannabinol (THC) as a source of carton. energy or anything else. The map positions in minutes of the the mutations are 18 minutes #2.4 5.0.7. 10, 11, 12, 13]. [42 minutes. M 1: 151 minutes: 391: 166 minutes: #8: (85 minutes: #141 fa) What is the minimum number of genes involved in THC utilization? OVA. AM 2 5 6 7 10 11 12 13 4 0 0 - 0 The nine linked mutations at 8' are crossed against one another by P1 transduction, with the results shown on the right I wild-type recombinants produced in the cross + 0 0 + 0 0 0 + + + 0 + 0 + + 0 + 0 + + 0 + + 0 + thc-2 (b) Which of these mutations behave like deletions? (C) Draw the best possible map of this region. Indicate uncertainties of order An F-prime element Recipient the allele on F-prime carrying the 8' the region the allele that the 15thc-16 the-17 is constructed and several new the the 4 + 0 0 + mutations (15, 16, 17) the 5 are isolated on the F- Mc-6 prime. The mutant thc.7 + + 0 0 episomes are used for the-10 complementation tests, thc-11 + the-12 0 + with the results shown on the 13 the right = growth on THC) (d) Which of these mutants are dominant? el Determine the number of genes represented by these the mutations and draw a revised map that shows the locations of gene borders. Clearly identity all remaining ambiguities in map order deletion endpoints, etc. This question deals with seven E. coli mutants (#1-7) that require leucine for growth. To map the mutations, transductional crosses 7 6 + 0 + 0 + + 5 + + + 4 0 + 0 3 2 1 + 0 0 + 0 0 2 3

Answer 1

- ciston-I: , 12,. cisponi: 4, 7, 10, 11, 12, cissor in 5,12, cistoon - 6,7, cisson : 7, 11, 13, osom-10,12, using above cisom 1- - - 2, 4, 5, 6, 7, 10, 11, 12, 13, so total a gene are required for utilization of THC de carbon source (6) Gene which are deletion – # 1, 3, 8, 9, 14 2 75 +6 Att 10 (d) e) in 10 Dominent gene the 15 as this showed maximam negabie (od grousher est gone which enter into recipient will be thet, the-17, the Is they that theistſ the-17 thelb

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