Question

Bi2030 (12) Defining the gene 36 1210 A set of 10 deletion mutants of some unspecified organism (#1-10) are tested for the production of 1 x2 4 x 10 ambiscity 2 x 7 5 x 8 wild-type recombinants when crossed pairwise to .2 x 10 5x9 12 7.1 O S one another. All tests are positive except those 3x6 5 x 10 shown on the night: 3x9 7 x 10 3 x 10 8x9 (a) Construct a map of these 10 deletions. Indicate 4 x 8 8 x 10 any ambiguities in relative orders or endpoints 4x99x 10 Seven point mutants (a-9) are each crossed to the above set of deletion mutants. Wild-type recombinants are produced in all crosses except: a x 8. 10; b x 1, 2; CX 6; d x 3, 9, 10; e x 2,7; fx 5, 9, 10; 9 x 1. notwildt (b) Determine the relative order of these seven point mutations and put them on your map. Indicate which map ambiguities they resolve. a b c d e f g Pairwise complementation tests between the seven point 9 + + + + + + 0 mutants are done with the results shown on the right fl + + + 0 + 0 [+ = phage produced): e O O + + 0 d + + + 0 (c) How many genes are most likely represented by these C + + 0 seven mutants? Draw gene borders on your map. b 0 0 a o 2. Eight riil mutants of phage T4 are isolated and crossed in all pairwise combinations to determine which mapping data 1 2 3 4 5 6 7 8 8 + 0 + + + 0 + 0 7 + + + + + 0 0

Answer 1

lo complementation test based on crossing with negative ohrome 1112131415r61718 19 110 100 + + + 200 + + 3 + + o + donlola +14 + 5 + + 0 + + + + 10+ 6T+ flo +to+to+too + + e lot loslal + + + + + + 1000 + a

I so gene sequence 1, 2, 3, 4, 5, 6, 7, 8, a76 9, 10, <3 4 Four - genes are mostly represented by these mutant gene I. a, b, e gene i d, F. gene Wine 7 ју: 8 Pope 1 gene order, TaR geres Genel РФ - 7 ney

Hope it's clear..thanks