Question

Succinate Dehydrogenase is an enzyme used to produce an intermediate product, fumerate, during the Krebs Cycle. The gene (DNA) that encodes succinate dehydrogenase is found on a chromosome in the nucleus. Fully describe the production, targeting/delivery and activity of succinate dehydrogenase by a cell. Give as many molecular details as you can. Be sure to include:

1. Where the gene is transcribed.

2. how the mRNA moves to the area where it will be translated,

3. how and where where the protein, succinate dehydrogenase, is translated

4. how the protein is targeted to the correct organelle (including information about the target sequence and the required translocon's for that organelle), and a hypothesis about any part of the cytoskeleton that might be used.

5. draw a figure illustrating the reaction that succinate dehydrogenase catalyzes, include a text description that shows you understand how enzymes work by explaining how succinate dehydrogenase catalyzes/speeds up this reaction, and explains the concept of coupled reactions and the importance of this coupled reaction indirectly to the production of ATP that occurs later.

6. Finally, give the details of how the ATP is made in mitochondria (using one product of that succinate dehydrogenase coupled reaction). Tell about the coupled reactions and how energy is released and used at various steps of the ETC during oxidative phosphorylation.

Succinate Dehydrogenase is an enzyme used to produce an intermediate product, fumerate, during the Krebs Cycle. The gene (DNA) that encodes succinate dehydrogenase is found on a chromosome in the nucleus. Fully describe the production, targeting/delivery and activity of succinate dehydrogenase by a cell. Give as many molecular details as you can. Be sure to include: 1. Where the gene is transcribed. 2. how the mRNA moves to the area where it will be translated, 3. how and where where the protein, succinate dehydrogenase, is translated 4. how the protein is targeted to the correct organelle (including information about the target sequence and the required translocon's for that organelle), and a hypothesis about any part of the cytoskeleton that might be used. 5. draw a figure illustrating the reaction that succinate dehydrogenase catalyzes, include a text description that shows you understand how enzymes work by explaining how succinate dehydrogenase catalyzes/speeds up this reaction, and explains the concept of coupled reactions and the importance of this coupled reaction indirectly to the production of ATP that occurs later. 6. Finally, give the details of how the ATP is made in mitochondria (using one product of that succinate dehydrogenase coupled reaction). Tell about the coupled reactions and how energy is released and used at various steps of the ETC during oxidative phosphorylation.

Answer 1

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