Homework Answers
1)
beta galactosidase induced
Lactose permease induced
all elements of genomic and plasmid lac operon are proper
2)
beta galactosidase induced
Lactose permease induced
genomic lac operon is inactive because of inactive promoter but the plasmid operon is normal and will induce both enzymes
3)
beta galactosidase non induced
Lactose permease induced
the mutation in structural gene can affect only that gene not the othe structural gene
4)
beta galactosidase induced
Lactose permease induced
the lacI gene is code for reppressor which is present in grenomic DNA but not in plasmid ;the genomic DNA operon is inactive cause the mutation in promoter sequence so this genomic opeon will give suffiecient repressor for plamid operon
5) beta galactosidase induced
Lactose permease induced
the genomic operon is normal but beta galactosidase synthsize constitutivelu cause lac oc mutation is dominant
6) beta galactosidase induced
Lactose permease induced
the enzymes will continue induced beacuse absense of repressor gene in plasmid
7)
beta galactosidase induced
Lactose permease induced
he genomic operon is normal
8)
beta galactosidase induced
Lactose permease induced
genomic operon is normal beta galactosidase constitutivly synthesized
9)
beta galactosidase induced
Lactose permease induced
plamid operon is normal beta galactosidase constitutivly synthesized
Add Answer to:
1. lacl Punt lacO* lacZ lacY/Flac Plast laco* lacZ lacr B-galactosidase activity? Non-Induced Induced Lactose permease...
A bacterium has the following genotype: lacIS lacP+ lacO- lacZ+ lacY- / lacI- lacP- lacO+ lacZ-...
A bacterium has the following genotype: lacIS lacP+ lacO- lacZ+ lacY- / lacI- lacP- lacO+ lacZ- lacY+ Fill in the blanks with either “on” or “off”. a. In the presence of lactose, transcription of β-galactosidase is __ON____. b. In the absence of lactose, transcription of β-galactosidase is ___ON___. c. In the presence of lactose, transcription of permease is __OFF____. d. In the absence of lactose, transcription of permease is __OFF____. Please give a detailed explanation....the mutations such as lacI^s are...
my teacher was useless with the method for doing these problems can someone please guide me...
my teacher was useless with the method for doing these
problems can someone please guide me in the right direction. dont
do all of them, but if you do at least explain how you came to that
conclusion. thank you
u
6. For E. coli strains with the lac genotypes show below, use a plus sign (+) to indicate the synthesis of B-galactosidase and permease and a minus sign (-) to indicate no synthesis of the proteins. Lactose absent Lactose...
Illustrate a conjugation event with an F’ cell that is lacIS lacP+lacO+lacZ- lacY-on the bacterial chromosome...
Illustrate a conjugation event with an F’ cell that is lacIS lacP+lacO+lacZ- lacY-on the bacterial chromosome and lacI- lacP+ lacOC lacZ+ lacY+ on an F plasmid with an F-cell that is lacI+ lacP+ lacO+ lacZ+ lacY-. Draw both cells before the conjugation event, as well as the recipient cell after conjugation occurs. Underneath all three cells, note whether the production of permease and beta-galactosidase would be high, basal, or none, and describe HOW the proteins and DNA sequences interact to...
which operon will make b-galactosidase even if lactose isn't present? p+ o+ lacZ+ lacI- p- Oc...
which operon will make b-galactosidase even if lactose
isn't present?
p+ o+ lacZ+ lacI-
p- Oc lacZ+ lacI-
p+ Oc lacZ- lacI+
p- o+ lacZ+ lacI+
p+ o+ lacZ+ lacI+
Oc = fails to bind R
regulatory promoter operator gene structural genes lacz lacY lacA β-galactosidase thiogalactoside transacetylase lactose permease
Background: Several genetic regions are needed for lactose metabolism: P(promoter), O(operator), lacZ(b-galactosidase gene), lacY(permease gene), lacA(transacetylase...
Background: Several genetic regions are needed for lactose
metabolism: P(promoter), O(operator), lacZ(b-galactosidase gene),
lacY(permease gene), lacA(transacetylase gene) and lacI(repressor
gene). Strains can be defined as having + - c or s copies: + refers
to the functional version, - refers to a defect version, c refers
to a constitutively active version and s refers to a superrepressor
version. Each strain may or may not have an extra-chromosomal
plasmid containing the genes indicated. TA=transacetylase
it would be very helpful if you...
A mutant E. coli strain is found that synthesizes B-galactosidase and permease but no B-galactoside-transacetylase in...
A mutant E. coli strain is found that synthesizes B-galactosidase and permease but no B-galactoside-transacetylase in the presence of lactose (or allolactose). What mutation(s) can lead to this outcome? The lac operon is shown here as a guide. zones where proteins bind DNA: genes and regulatory sequences I lacl promoter operator lac Z l ac Y La repressor B-galactosidase B-galactoside transacetylase proteins Operator Laci promoter Lac Y Lac A Lacz
for 1-5 define those The Lac operon is an inducible set of genes found in bacteria...
for
1-5 define those
The Lac operon is an inducible set of genes found in bacteria cells that helps the bacteria to metabolize the disaccharide lactose. When it is turned on it produces proteins that pump lactose into the bacteria cell and break it down into glucose and galactose, which can then be used by the bacteria as a source of energy The two figures below show the Lac Operon along with the lacl gene (which regulates the Lac operon...
my teacher was useless with the method for doing these
problems can someone please guide me in the right direction. dont
do all of them, but if you do at least explain how you came to that
conclusion. thank you
u
6. For E. coli strains with the lac genotypes show below, use a plus sign (+) to indicate the synthesis of B-galactosidase and permease and a minus sign (-) to indicate no synthesis of the proteins. Lactose absent Lactose...
which operon will make b-galactosidase even if lactose
isn't present?
p+ o+ lacZ+ lacI-
p- Oc lacZ+ lacI-
p+ Oc lacZ- lacI+
p- o+ lacZ+ lacI+
p+ o+ lacZ+ lacI+
Oc = fails to bind R
regulatory promoter operator gene structural genes lacz lacY lacA β-galactosidase thiogalactoside transacetylase lactose permease
Background: Several genetic regions are needed for lactose
metabolism: P(promoter), O(operator), lacZ(b-galactosidase gene),
lacY(permease gene), lacA(transacetylase gene) and lacI(repressor
gene). Strains can be defined as having + - c or s copies: + refers
to the functional version, - refers to a defect version, c refers
to a constitutively active version and s refers to a superrepressor
version. Each strain may or may not have an extra-chromosomal
plasmid containing the genes indicated. TA=transacetylase
it would be very helpful if you...
A mutant E. coli strain is found that synthesizes B-galactosidase and permease but no B-galactoside-transacetylase in the presence of lactose (or allolactose). What mutation(s) can lead to this outcome? The lac operon is shown here as a guide. zones where proteins bind DNA: genes and regulatory sequences I lacl promoter operator lac Z l ac Y La repressor B-galactosidase B-galactoside transacetylase proteins Operator Laci promoter Lac Y Lac A Lacz
for
1-5 define those
The Lac operon is an inducible set of genes found in bacteria cells that helps the bacteria to metabolize the disaccharide lactose. When it is turned on it produces proteins that pump lactose into the bacteria cell and break it down into glucose and galactose, which can then be used by the bacteria as a source of energy The two figures below show the Lac Operon along with the lacl gene (which regulates the Lac operon...
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