Question

avant- Piste: 1 2 3 4 5 6 7 5400 bp=> 4400 bp 4000 bp | 3200 bp 1400 bp- 1200 bp 1000 bp 800 bp-> | | 200 bp=> | |

1: EcoRI
2: BamHI
3:PstI
4:EcoRI + BamHI
5:EcoRI + PstI
6:BamHI + PstI
7: EcoRI + BamHI + PstI
Draw a restriction map of this plasmid.

To determine the restriction map of a plasmid, you digest it with one or more of the three restriction enzymes EcoRI BamHI and PstI. Sfter digestion, you anslyzr the size of the fragments obtained on electrophoresis gel and you obtain the following gel

avant- Piste: 1 2 3 4 5 6 7 5400 bp=> 4400 bp 4000 bp | 3200 bp 1400 bp- 1200 bp 1000 bp 800 bp-> | | 200 bp=> | |

piste1: EcoRI: 5400 bp
piste2: BamHI: 5400bp
piste3:PstlI: 4400bp , 1000bp
piste4: EcoRI + BamHI: 4000bp, 1400bp
piste5: Ecori + PstI: 4400bp, 800bp, 200bp
piste6:BamHI + PstI: 3200bp, 1200bp, 1000bp
piste7: EcoRI + BamHI + PstI: 3200bp, 1200bp, 800bp, 200bp
Q what's the restriction map of this plasmid?

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Answer #1

Ans: II Please Give Ratings II

piste1: EcoRI: 5400 bp
piste2: BamHI: 5400bp
piste3:PstlI: 4400bp , 1000bp
piste4: EcoRI + BamHI: 4000bp, 1400bp
piste5: Ecori + PstI: 4400bp, 800bp, 200bp
piste6:BamHI + PstI: 3200bp, 1200bp, 1000bp
piste7: EcoRI + BamHI + PstI: 3200bp, 1200bp, 800bp, 200bp

EcoRI : F4 деење 5.4 kb Es, к Вам нт : 54 » Palil : 4:4 , 1-ок» 44 — 0 — ECORI + BamHI & 4.0kb, 14Kb ECORI+ Pst: 4400 bp 4.4k

EcoRs + pstit diguinon; : ) Bam HI + Pst + esti digestions 4.4 :.. The acest auction map of this plasmid; Pstr borr - Psts X

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