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4. Explain the design and rationale of the ELISA plate,...
1. What does an ELISA test for? 2. What variables can affect the outcome? (Name 4)...
1. What does an ELISA test for? 2. What variables can affect the outcome? (Name 4) 3. What are the three important limitations of an ELISA? Explain each. 4. In the virtual lab, the ELISA plate has been pretreated to bind SLE antigen to each well. WHY? How do you expect the assay setup to differ in our case where the antigen of interest is present in a heterogenous sample in small quantities? 5. Why are there both a positive...
ELISA-HIV Testing DATA SHEET RESULTS For each of the following, report the color and indicate whether...
ELISA-HIV Testing DATA SHEET RESULTS For each of the following, report the color and indicate whether the result was positive or negative. Neg.control: negative Donorl: negative Pos. control: positive Donor 2: positive Based on your results, which donor has been exposed to HIV? Donor 2 QUESTIONS 1 Describe the purpose of the two controls. The purpose of the two controls is to show what the positive and negative would look like, and it also helps determine it the experiment was...
Please help with all questions with regards to ELISA 1. Explain how one would go about preparing ...
Please help with all questions with regards to ELISA 1. Explain how one would go about preparing mouse anti-Tomato Spotted Wilt antiserum (5). 2. Explain how one would go about preparing a goat anti-mouse-Fc = alkaline phosphatase conjugate (9). 3. Please explain how to prepare a 1:20 dilution, showing an example (3) 4. Why is it necessary to wash the wells between each step (2) 5. Why was a goat anti-rabbit Fc specific conjugate used in the ELISA, rather than...
Directions: Please complete Prelecture Assignment and upload answers to Carmen as a PDF before coming to...
Directions: Please complete Prelecture Assignment and upload answers to Carmen as a PDF before coming to lecture. Before attempting this worksheet, please read Lab 8 as this worksheet accompanies the lab and helps you get your lab notebook ready by guiding you through the necessary calculations that will be needed to start the experiment. This lab takes more thought and effort to set-up, but faster to complete if you think about things now. Please bring a copy of the completed...
*Microbiology. Please answer/draw the below question. Thank you! Name Plate Count: Problem #5 Дото си иoтeтo...
*Microbiology. Please answer/draw the below question. Thank
you!
Name Plate Count: Problem #5 Дото си иoтeтo oтo A A microbiologist performs quality control on water samples from Crystal Clear Bottled Water Inc.be The Quality Control inspector wants the following dilutions to be plated in triplicate: 1:100,000 1:1,000,000 1:10,000,000 1:100,000,000 on bere the one ont osa You have 9 mL deionized water dilution blanks you can use to create your serial dilutions. a. Draw out a serial dilution diagram. Show how...
Hi, I need help with this question. Please explain in details how you got the answer...
Hi, I need help with this question. Please explain in details
how you got the answer with steps shown for each part so I can
understand it thank you. I am not sure why 2.9 is wrong.
How long does it take you to get from home to work? Here are the travel times in minutes for 15 workers in North Carolina, chosen at random by the Census Bureau 30 20 10 40 25 20 10 62 15 40 1...
Java Programming. please give me answer and explain in details. Question 1 (c) (4 marks] For...
Java Programming.
please give me answer and explain in details.
Question 1 (c) (4 marks] For each of the methods equals and hashCode in the following class, explain whether or not they are implemented correctly (with respect to their specification in the object class and any additional specification in this class). If they are not implemented correctly. show how they may be rewritten to address the problems that you have identified. *An immutable representation of a term in a polynomial....
I answered 4.a as C- 15-crown-5, is that correct? If not, what is and please explain how you got the answer. Trying to f...
I answered 4.a as C-
15-crown-5, is that correct? If not, what is and please explain how
you got the answer. Trying to figure 4.a 4.b and 5 out....
4. Identify the missing reagent needed to carry out the following transformation. NaF Benzene Br A) 12-crown-5 B) 12-crown-4 C) 15-crown-5 D) 18-crown-6 E) none of these 4. Provide the reagents necessary to prepare the following compound using alkoxymercuration . demercuration. Answer: 5. Explain why long-term storage of ethers can be...
Please explain part A in details thx! Question 3 An n'pn Si BJT is shown in Figure Q3(a). The emitter is heavily doped with 1020 cm3 whereas the base and collector are lightly doped with 5x1018 a...
Please explain part A in details thx!
Question 3 An n'pn Si BJT is shown in Figure Q3(a). The emitter is heavily doped with 1020 cm3 whereas the base and collector are lightly doped with 5x1018 and 3x1018, respectively. The lengths of emitter, base, and collector are 0.5um, 0.2um, and 0.5 um.. The dielectric constant of silicon is 11.8 and the intrinsic carrier concentration at 300 K is 1.5x1010 cm3. Assume that a 0.026 eV at 300 K. 0.99, e...
Please choose 5 questions from 20 and answer them. 1. How can a security framework assist...
Please choose 5 questions from 20 and answer them.
1. How can a security framework assist in the design and implementation of a security infrastructure? What is information security governance? Who in the organization should plan for it? 2. Where can a security administrator find information on established security frameworks? 3. What is the ISO 27000 series of standards? Which individual standards make up the series? 4. What are the issues associated with adopting a formal framework or model? 5....
ELISA-HIV Testing DATA SHEET RESULTS For each of the following, report the color and indicate whether the result was positive or negative. Neg.control: negative Donorl: negative Pos. control: positive Donor 2: positive Based on your results, which donor has been exposed to HIV? Donor 2 QUESTIONS 1 Describe the purpose of the two controls. The purpose of the two controls is to show what the positive and negative would look like, and it also helps determine it the experiment was...
Directions: Please complete Prelecture Assignment and upload answers to Carmen as a PDF before coming to lecture. Before attempting this worksheet, please read Lab 8 as this worksheet accompanies the lab and helps you get your lab notebook ready by guiding you through the necessary calculations that will be needed to start the experiment. This lab takes more thought and effort to set-up, but faster to complete if you think about things now. Please bring a copy of the completed...
*Microbiology. Please answer/draw the below question. Thank
you!
Name Plate Count: Problem #5 Дото си иoтeтo oтo A A microbiologist performs quality control on water samples from Crystal Clear Bottled Water Inc.be The Quality Control inspector wants the following dilutions to be plated in triplicate: 1:100,000 1:1,000,000 1:10,000,000 1:100,000,000 on bere the one ont osa You have 9 mL deionized water dilution blanks you can use to create your serial dilutions. a. Draw out a serial dilution diagram. Show how...
Hi, I need help with this question. Please explain in details
how you got the answer with steps shown for each part so I can
understand it thank you. I am not sure why 2.9 is wrong.
How long does it take you to get from home to work? Here are the travel times in minutes for 15 workers in North Carolina, chosen at random by the Census Bureau 30 20 10 40 25 20 10 62 15 40 1...
Java Programming.
please give me answer and explain in details.
Question 1 (c) (4 marks] For each of the methods equals and hashCode in the following class, explain whether or not they are implemented correctly (with respect to their specification in the object class and any additional specification in this class). If they are not implemented correctly. show how they may be rewritten to address the problems that you have identified. *An immutable representation of a term in a polynomial....
I answered 4.a as C-
15-crown-5, is that correct? If not, what is and please explain how
you got the answer. Trying to figure 4.a 4.b and 5 out....
4. Identify the missing reagent needed to carry out the following transformation. NaF Benzene Br A) 12-crown-5 B) 12-crown-4 C) 15-crown-5 D) 18-crown-6 E) none of these 4. Provide the reagents necessary to prepare the following compound using alkoxymercuration . demercuration. Answer: 5. Explain why long-term storage of ethers can be...
Please explain part A in details thx!
Question 3 An n'pn Si BJT is shown in Figure Q3(a). The emitter is heavily doped with 1020 cm3 whereas the base and collector are lightly doped with 5x1018 and 3x1018, respectively. The lengths of emitter, base, and collector are 0.5um, 0.2um, and 0.5 um.. The dielectric constant of silicon is 11.8 and the intrinsic carrier concentration at 300 K is 1.5x1010 cm3. Assume that a 0.026 eV at 300 K. 0.99, e...
Please choose 5 questions from 20 and answer them.
1. How can a security framework assist in the design and implementation of a security infrastructure? What is information security governance? Who in the organization should plan for it? 2. Where can a security administrator find information on established security frameworks? 3. What is the ISO 27000 series of standards? Which individual standards make up the series? 4. What are the issues associated with adopting a formal framework or model? 5....
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