DISCUSSION Numer specie 2. What would Baril look like with an acid fast stain! 3. What...
Hi, LaurenSign OutA Home AB-Structural Stains croLab Tutor: Use and Application of the Acid-Fast Stain K2 of 3 You INCORRECTLY perform an acid-fast stain on a smear from a mixed culture of Mycobacterium that you forget to use acid-alcohol during the procedure If you viewed this slide with a microscope smegmatis (acid-fast cells) and Staphylococcus aureus (non-acid-fast cells) Your mistake is what would each cell type look like? View Available Hint(s) M smegmatis cells would appear red S aureus cells...
Lab Supplemental Questions hital Questions Acid-Fast Stain 1. Why is acid-alcohol used as the decoloriting agent in the dio Aud-alcohol is used as the decolorizing Staining because it ensures that the pro form non acld fast organisme. agent in the acid-fast stain? orizing agent in the acid-fast the primary stain is removed 2. Why don't we Gram stain the mycobacterium? stain both acid-fast and non-acid-fast bacteria stain red, what 3. If at the end of the acid-fast stain both acid-fast...
LAB REPORT 2- Selected questions from Exercise 3-7 (Acid-fast stain) Note: This is turned in after you have viewed your acid fast stain slides. Bacteria for this exercise are: Mycobacterium smegmatis (or M. phlei) and S. aureus. Q1. Acid fast bacteria are have some significant differences as compared to non-acid fast bacteria. What are the major chemical (structural) differences among these different bacteria that might explain the acid-fast stain? Hint: This structural difference also affects the way the bacteria grow...
MICROBIOLOGY: Exercise 3-7 Acid-Fast Stain (Kinyoun Method TRUE FALSE 1. The Acid-Fast stain is a differential stain. 2. The counter stain for the Kinyoun Acid-Fast method is 3. The decolorizing agent for the Kinyoun Acid-Fast method is . The Kinyoun method requires heat for at least 5 minutes . An Acid-Fast microbe is 6. The Acid-Fast method is used primarily to identify microbes in the genus TRUE FALSE in color 7. What is the other type of acid-fast staining procedure?...
What color would ine bacterial cells appear if the patient has tuberculosis? and capsule stains The only difference is that without heat you have to stain the smear with carbol fuschin for 10 1. What is chemically unique about the Mycobacterium genus that personal Note: Alternate cold method acid fast stain (Kinyoun) minutes My note: There is not only one difference. The counterstain here is brilliant green rather than methylene blue And to allow the carbol fuchsin to penetrate acid...
In the acid-fast stain, the purpose of the methylene blue is to __________. (1) remove the stain from non acid-fast cells (2) kill bacteria (3) stain non-acid-fast cells (4) disinfect the slide
What two historically important diseases are caused by acid fast bacteria? Give the scientific name for each of the organisms that cause these diseases. 2. 3. How does acid-fast stain detect mycolic acids? 4. Are there species from Mycobacterium genus that are part of the normal flora? If so, write the name and function.
Questions What does the term "Acid Fa" mean? Are acid-Fast organisms Gram-positive or Gram- 2 Do your results indicate that endospores are acid-fast? Explain. 3 Why is it best to use "old" cultures for this staining procedure? 4 When you stain your Mycobacterium smegmatis, some of the cells are red and others are blue. What does tell you about your culture?
69. THE ENDOSPORE itself is might to stain_?_ color in an ACID FAST stain. (a) HOT pink (c) purple (d) green (e) baby-blue 70. All STAINS begin with a properly prepared _?_ . (a) dye (b) slide (c) smear (d) dog (e) cat 71. Which of the following is an ENDOTOXIN found in some microbes? This is results in fever, blood vessel dilation and possibly SHOCK when it is released into the human blood stream? (a) the plasma membrane (b)...
What result would you expect if you were to perform an endospore stain on an acid-fast bacterium?