Homework Answers
C) Yeast cells are Gram postive as it appears in purple color when observed after gram staining. Yeast appears as single cells containing buds and sometimes as hyphae (plant like branches) appears.
D) The main principle behind Gram’s staining is that the primary violet stain color all the cells purple without considering the composition of cell wall. When iodine is added the cell wall turns to purple. As Yeast cells does not contain any lipid layer and after that when decolourization is performed using 95% alcohol and safranine is added no reaction occurs and they remain in purple color and gives a gram postive reaction. Unless in bacteria they have lipopolysaccharide layer. When the cell wall permeability increases during decolorization process all the crystal violet which was taken up by the cell releases out and is washed off. After addition of Safranine the lipid layer takes up the pink color and thus bactria appears to be gram negative by nature. Thus the basic difference in the pattern of yeast and bacteria in staining is because of lipid layer which is absent in yeast and is present in bacteria.
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C) What is the color and shape of the yeast cells after Gram...
Pre-Lab Questions: After staining what color will Gram positive bacteria be? What color will Gram negative...
Pre-Lab Questions: After staining what color will Gram positive bacteria be? What color will Gram negative bacteria be? What structure is more prominent in Gram positive bacteria than Gram negative bacteria? Why should I not spray water aggressively or directly on my bacteria on my slide?
Describe the color of Gram positive and Gram negative bacteria in each step of the Gram...
Describe the color of Gram positive and Gram negative bacteria in each step of the Gram staining procedure. (Be sure to account for the procedure, the reagent, and the colors of Gram negative and Gram positive bacteria in your description.)
1. Describe several advantages of differential staining procedures compared with simple staining techniques. 2. Give the...
1. Describe several advantages of differential staining procedures compared with simple staining techniques. 2. Give the purpose of each of the following reagents in a differential staining procedure: a. Primary stain b. Counter stain c. Decolorizing agent d. Mordant 3. Why is it important for the counter stain to be a lighter color than the primary stain? 4. You have done gram staining for a gram positive bacteria, but at the end you observed pinkish-red bacteria under microscope, what was...
SWAU u sulide of the slide Questions 1. What color are Gram-positive cells supposed to be...
SWAU u sulide of the slide Questions 1. What color are Gram-positive cells supposed to be after a Gram-stain? What about Gram-negative cells? (2 pts.) a rum + - purple Gram -- pink 2. What characteristics can be determined in a Gram-stain? (2 pts) 3. List all of the things that can go happen during the Gram-staining process that could lead to an incorrect or poor result. (2pts.) 4. Why are direct gram stains ordered on clinical specimens? (2pts.) 5....
Steps of Gram Staining Bacteria containing thick cell wall Violet color Bacteria containing thin cell wall...
Steps of Gram Staining Bacteria containing thick cell wall Violet color Bacteria containing thin cell wall (LPS) Violet color Crystal violet treatment lodine Purple color Purple color Decolorization Purple color colorless Safranin Purple color pink 3. A fellow student showed you a gram stained slide where cells containing thick cell walls were stained pink. What would you tell her about the staining procedure? Why? 4. A fellow student showed you a gram stained slide where cells containing LPS were stained...
43.Lipopolysaccharides (LPS) are found in: a. Gram-positive bacteria b. Gram-negative bacteria c. all Bacteria d. Archaea...
43.Lipopolysaccharides (LPS) are found in: a. Gram-positive bacteria b. Gram-negative bacteria c. all Bacteria d. Archaea e. Eukaryotes ------------------------- 44. There are similarities and differences between Gram-negative and Gram-positive bacteria. Which of the following is not true? a. The cell wall of Gram-negative cells is composed of only one or two layers of peptidoglycan whereas the cell walls of Grampositive bacteria have many peptidoglycan layers b. Only Gram-positive bacterial cells have a periplasm 2/6/18 8 c. The cell membranes of...
QUESTIONS 1) Are acid fast cells gram positive or gram negative? Explain why it might be...
QUESTIONS 1) Are acid fast cells gram positive or gram negative? Explain why it might be difficult to determine the Gram reaction of acid fast cells. 2) What is the significance of the mycolic acid layer in the following contexts? A) To bacteria for survival and infection B) During the acid fast staining procedure QUESTIONS 1) What is the difference between a pure culture and a pure colony? Is a streaking for isolation plate a pure culture when your original...
NEED HELP WITH ALL PLEASE. MICROBIOLOGY You have stained a bacteria using the Gram staining technique...
NEED HELP WITH ALL PLEASE. MICROBIOLOGY
You have stained a bacteria using the Gram staining technique what color would any endospores be if present? A. Colorless B. Green C. Pink D. Blue E. Purple 2. If you omitted the Safranin step in a gram staining procedure, what would be the color of a Gram-negative bacteria? A. Colorless B. Pink C. Green D. Purple E. Blue 3. Bacillus licheniformis would be what color and shape after a Gram staining? A. Purple...
EXPERIMENT 1: Which bacteria was gram-positive after Gram staining? Select one: O a. S. aureus O...
EXPERIMENT 1: Which bacteria was gram-positive after Gram staining? Select one: O a. S. aureus O b. S. pneumoniae o cnone of the answer choices o d. E. coli EXPERIMENT 1: Which bacteria was pink and rod-shaped after Gram staining? Select one: O a. S. pneumoniae o b. E. coli O c. M. phlei O d. S. aureus EXPERIMENT 2: Which bacteria did not stain green after acid-fast staining? Select one: O a. E. coli O b. M. phlei O...
1. Please describe the gram staining procedure. Be sure to include: crystal violet, iodine, alcohol, safranin,...
1. Please describe the gram staining procedure. Be sure to include: crystal violet, iodine, alcohol, safranin, and any other terms you think are important to the technique. You do not need to re- write the lab procedure here please describe what is occurring conceptually to both gram + and gram-cells during the procedure. What would a gram positive cell look like under oil immersion if a researcher forgot to use crystal violet? Please provide a rationale for your response. 3....
Describe the color of Gram positive and Gram negative bacteria in each step of the Gram staining procedure. (Be sure to account for the procedure, the reagent, and the colors of Gram negative and Gram positive bacteria in your description.)
1. Describe several advantages of differential staining procedures compared with simple staining techniques. 2. Give the purpose of each of the following reagents in a differential staining procedure: a. Primary stain b. Counter stain c. Decolorizing agent d. Mordant 3. Why is it important for the counter stain to be a lighter color than the primary stain? 4. You have done gram staining for a gram positive bacteria, but at the end you observed pinkish-red bacteria under microscope, what was...
SWAU u sulide of the slide Questions 1. What color are Gram-positive cells supposed to be after a Gram-stain? What about Gram-negative cells? (2 pts.) a rum + - purple Gram -- pink 2. What characteristics can be determined in a Gram-stain? (2 pts) 3. List all of the things that can go happen during the Gram-staining process that could lead to an incorrect or poor result. (2pts.) 4. Why are direct gram stains ordered on clinical specimens? (2pts.) 5....
Steps of Gram Staining Bacteria containing thick cell wall Violet color Bacteria containing thin cell wall (LPS) Violet color Crystal violet treatment lodine Purple color Purple color Decolorization Purple color colorless Safranin Purple color pink 3. A fellow student showed you a gram stained slide where cells containing thick cell walls were stained pink. What would you tell her about the staining procedure? Why? 4. A fellow student showed you a gram stained slide where cells containing LPS were stained...
QUESTIONS 1) Are acid fast cells gram positive or gram negative? Explain why it might be difficult to determine the Gram reaction of acid fast cells. 2) What is the significance of the mycolic acid layer in the following contexts? A) To bacteria for survival and infection B) During the acid fast staining procedure QUESTIONS 1) What is the difference between a pure culture and a pure colony? Is a streaking for isolation plate a pure culture when your original...
NEED HELP WITH ALL PLEASE. MICROBIOLOGY
You have stained a bacteria using the Gram staining technique what color would any endospores be if present? A. Colorless B. Green C. Pink D. Blue E. Purple 2. If you omitted the Safranin step in a gram staining procedure, what would be the color of a Gram-negative bacteria? A. Colorless B. Pink C. Green D. Purple E. Blue 3. Bacillus licheniformis would be what color and shape after a Gram staining? A. Purple...
EXPERIMENT 1: Which bacteria was gram-positive after Gram staining? Select one: O a. S. aureus O b. S. pneumoniae o cnone of the answer choices o d. E. coli EXPERIMENT 1: Which bacteria was pink and rod-shaped after Gram staining? Select one: O a. S. pneumoniae o b. E. coli O c. M. phlei O d. S. aureus EXPERIMENT 2: Which bacteria did not stain green after acid-fast staining? Select one: O a. E. coli O b. M. phlei O...
1. Please describe the gram staining procedure. Be sure to include: crystal violet, iodine, alcohol, safranin, and any other terms you think are important to the technique. You do not need to re- write the lab procedure here please describe what is occurring conceptually to both gram + and gram-cells during the procedure. What would a gram positive cell look like under oil immersion if a researcher forgot to use crystal violet? Please provide a rationale for your response. 3....
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