Question

DNA size DNA standards sample wells into which samples were loaded 10000 8000 6000 4000 2000 1000 500

simply by comparing the mobility of the fragments in the digested plasmid to the fragments in the DNA size standard, what is the approximate size of each of the fragments?

Answer 1

DNA size standard has 7 bands of size 10000, 8000, 6000,4000, 2000, 1000, 500. These will travel distance on the basis of their size in gel matrix during electrophoresis. First calculate the distance traveled by each band (fragment) standard as well as sample from sample well.

BpDNA	Distance mm
10000	27
8000	29
6000	33
4000	39
2000	52
1000	72
500	88
B1	38
B2	71

Distance mm Distance (mm) 2000 4000 8 000 10000 12000 6000 DNA Size (bp) • Distance mm ... .. Log. (Distance mm)

Size of sample bands are marked with red points. Based on this, approximate size of sample bands will be

B1- 4300bp,

B2- 1050bp (traveled slightly less distance than 1000bp band, so zize will be slightly higher than 1000bp)

Did this help? If yes, do give positive rating for this answer