If you digested your vector with BamHI (G^GATCC), which of the following restriction enzymes could you...

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Question

If you digested your vector with BamHI (G^GATCC), which of the following restriction enzymes could you...

If you digested your vector with BamHI (G^GATCC), which of the following restriction
enzymes could you use to digest genomic DNA to generate a library?

a. Acc65I (G^GTACC)
b. BglII (A^GATCC)
c. Sau3AI (^GATC)
d. a, b and c
e. b and c

science biology

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Answer 1

Answer #1

BamHI results in a 5' G-GATCC 3' -G GATCC-

3' CCTAG-G 5' $\rightarrow$ -CCTAG G-

Sau3AI results in a 5' NGATCN 3' -N GATCN-

3' NCTAGN 5' $\rightarrow$ -NCTAG N-

As Sau3AI produces same sticky ends as BamHI , so Sau3AI will be used to digest genomic DNA to generate a library.

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Answer 2

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