A linear DNA molecule was digested with restriction enzyme EcoR I (E) and three fragments were...
A linear DNA molecule was digested with restriction enzyme EcoR I (E) and three fragments were produced of sizes 7.3 kb, 4.0 kb and 3.4 kb. The restriction enzyme BamH I (B) produced three fragments of sizes 7.9 kb, 4.8 kb and 2 kb. When the two enzymes EcoR I and BamH I were used together, five fragments were produced of sizes 6.5kb, 4kb, 2kb, 1.4kb, and 0.8kb. Which of the following is the correct restriction map on this molecule
A. A: 7.3kb E 4.8kb B 2kb B 4kb E
B. 4kb E 0.8kb B 6.5kb E 1.4kb B 2kb
C. 6.5kb B 4kb E 2kb E 1.4kb B 0.4kb
D. 6.5kb E 4kb E 2kb B 1.4kb B 0.4kb
E. 4kb B 0.8kb E 6.5kb B 1.4kb E 2kb
Homework Answers
The correct option is B
Add Answer to:
A linear DNA molecule was digested with restriction enzyme EcoR
I (E) and three fragments were...
A 10 kb linear DNA molecule was digested with restriction enzyme EcoRI (E) and two fragments...
A 10 kb linear DNA molecule was digested with restriction enzyme EcoRI (E) and two fragments were produced of sizes 6 kb and 4 kb. The restriction enzyme HaeIII (H) also produced two fragments of sizes 9 kb and 1 kb. When the two enzymes HaeIII and EcoRI were used together, three fragments were produced of sizes 1 kb, 3 kb, and 6 kb. A 4 kb long cloned genomic probe from this region hybridized to the 3 kb and...
A linear DNA molecule that is 3250 bp long is digested with restriction enzyme A alone,...
A linear DNA molecule that is 3250 bp long is digested with restriction enzyme A alone, restriction enzyme B alone, or a combination of enzymes A and B to produce the following fragment sizes. Construct a restriction map of the DNA fragment. How many times does enzyme A cut the linear DNA molecule? If you are unable to place both enzymes A and B on the map, show one possible arrangement of enzyme A cognition sites on the DNA molecule.
5. You have a linear DNA fragment and you wish to generate a restriction map using...
5. You have a linear DNA fragment and you wish to generate a restriction map using Mstl and EcoR1. When the framgent is digested with Mst1 and the DNA is run on a gel you observe a 4kb and a 6kb fragment. When the DNA is digested with EcoR1, 1kb, 4kb and 5kb fragments are generated, A double digest using both enzymes produce 1kb, 2kb, 3kb and 4kb fragments. Explain these results and draw a restirction map consistent with these...
2) A linear phage chromosome is labelled at both ends with 32P and digested with restriction...
2) A linear phage chromosome is labelled at both ends with 32P and digested with restriction enzymes. EcoRI produces fragments of sizes 2.9, 4.5. 6.2.7.4, and 8.0 kb. An autoradiogram developed from a Southern blot of this digest shows radioactivity associated with the 6.2 and 8.0 fragments. BamHI cleaves the same molecule into fragments of sizes 6.0, 10.1 and 12.9; the label is found to be associated with the 6.0 and 10.1 fragments. When EcoRI and BamHI are used together,...
Restriction Mapping of a Linear DNA • 2 DNA is a linear and double-stranded DNA isolated...
Restriction Mapping of a Linear DNA • 2 DNA is a linear and double-stranded DNA isolated from bacteriophage lambda. This bacteriophage is a virus that infects E. coli and can undergo specialized transduction (see the transduction lab lecture). Assuming you obtained the following fragments from the restriction digestion. Draw the restriction map for the EcoRI and Hindi II enzymes in the 2. DNA. Fragment Size EcoRI 15300 6200 5250 2100 EcoRI + HindIII 15300 4400 4000 2100 1800 Hind!!! 17100...
The figure below shows a restriction map of a segment of a DNA molecule. Eco refers...
The figure below shows a restriction map of a segment of a DNA molecule. Eco refers to locations where the restriction endonuclease EcoRI cuts the DNA, and Pst refers to locations where the restriction enzyme Pst cuts the DNA. Potential restriction sites are numbered 1-6. Distances between restriction sites are shown on the bottom scale in base pairs (bp). The thick line represents the part of the molecule that has homology with a probe. Eco Pst Eco Pst Eco Pst...
I need help making a restriction digest map for both of these sets of fragments. Please...
I need help making a
restriction digest map for both of these sets of fragments. Please
explain the steps and include drawing.
Draw restriction maps for the plasmids that produced the following fragments from restriction digestion: Digest Performed Size of DNA fragments produced BamHI BamHI/HindI 2.5kb & 1.5kb BamHI/EcoRI 3kb & 1kb HindIlI/EcoRI 3.5kb &0.5kb 4kp Digest Performed Size of DNA fragments produced BamHI BamHI & Sal1 4kb, 5kb BamHI & Pst1 6kb& 3kb Sal1&Pst1 1kb& 8kb kb
Hi I have a problem with number 5, it involves gel analysis results. There are 2 parts, a,b,c. For A Im sure you need to make a graph with distance in (cm) on the vertical axis and log10 bp on the hor...
Hi I have a problem with number 5, it involves gel
analysis results. There are 2 parts, a,b,c. For A Im sure you need
to make a graph with distance in (cm) on the vertical axis and
log10 bp on the horitzontal. I need help figuring out where to
start and what to do. Please help!
The following question will provide practice in interpreting and analyzing gel results You obtained the DNA electrophoresis gel below. Three samples of lambda phage...
Restriction Mapping Below is a restriction map for the plasmid PGEN101 (total length - 20 kb)....
Restriction Mapping Below is a restriction map for the plasmid PGEN101 (total length - 20 kb). Using this map as a guide, give the number of restriction fragments along with their associated lengths that would result from digesting PGEN101 with the restriction enzymes EcoRI, BamHII, anda combination of EcoRI + BamHI. BamHI BamHI BamHI / PGEN101 (20 kb) Mb EcoRI Digest Performed Size Emments Obtained EcoRI........ BamHI.. EcoRI + BamHI.... Two freshmen college students, interested in becoming gene jocks, performed...
Aliquots of a 7.8-kilobase (kb) linear piece of DNA are digested with the restriction enzymes Pvu...
Aliquots of a 7.8-kilobase (kb) linear piece of DNA are digested with the restriction enzymes PvuII, HincII, ClaI, and BanII, alone and in pairs. The digestion products are separated by gel electrophoresis, and the size of each fragment is determined by comparison to size standards. The fragment sizes obtained, in kilobase pairs, are given below. Draw a diagram of the 7.8-kb fragment, showing the location of the restriction sites for each enzyme. a.Digestion with PvuII: 1.3 kb, 6.5 kb b.Digestion...
A linear DNA molecule that is 3250 bp long is digested with restriction enzyme A alone, restriction enzyme B alone, or a combination of enzymes A and B to produce the following fragment sizes. Construct a restriction map of the DNA fragment. How many times does enzyme A cut the linear DNA molecule? If you are unable to place both enzymes A and B on the map, show one possible arrangement of enzyme A cognition sites on the DNA molecule.
5. You have a linear DNA fragment and you wish to generate a restriction map using Mstl and EcoR1. When the framgent is digested with Mst1 and the DNA is run on a gel you observe a 4kb and a 6kb fragment. When the DNA is digested with EcoR1, 1kb, 4kb and 5kb fragments are generated, A double digest using both enzymes produce 1kb, 2kb, 3kb and 4kb fragments. Explain these results and draw a restirction map consistent with these...
2) A linear phage chromosome is labelled at both ends with 32P and digested with restriction enzymes. EcoRI produces fragments of sizes 2.9, 4.5. 6.2.7.4, and 8.0 kb. An autoradiogram developed from a Southern blot of this digest shows radioactivity associated with the 6.2 and 8.0 fragments. BamHI cleaves the same molecule into fragments of sizes 6.0, 10.1 and 12.9; the label is found to be associated with the 6.0 and 10.1 fragments. When EcoRI and BamHI are used together,...
Restriction Mapping of a Linear DNA • 2 DNA is a linear and double-stranded DNA isolated from bacteriophage lambda. This bacteriophage is a virus that infects E. coli and can undergo specialized transduction (see the transduction lab lecture). Assuming you obtained the following fragments from the restriction digestion. Draw the restriction map for the EcoRI and Hindi II enzymes in the 2. DNA. Fragment Size EcoRI 15300 6200 5250 2100 EcoRI + HindIII 15300 4400 4000 2100 1800 Hind!!! 17100...
The figure below shows a restriction map of a segment of a DNA molecule. Eco refers to locations where the restriction endonuclease EcoRI cuts the DNA, and Pst refers to locations where the restriction enzyme Pst cuts the DNA. Potential restriction sites are numbered 1-6. Distances between restriction sites are shown on the bottom scale in base pairs (bp). The thick line represents the part of the molecule that has homology with a probe. Eco Pst Eco Pst Eco Pst...
I need help making a
restriction digest map for both of these sets of fragments. Please
explain the steps and include drawing.
Draw restriction maps for the plasmids that produced the following fragments from restriction digestion: Digest Performed Size of DNA fragments produced BamHI BamHI/HindI 2.5kb & 1.5kb BamHI/EcoRI 3kb & 1kb HindIlI/EcoRI 3.5kb &0.5kb 4kp Digest Performed Size of DNA fragments produced BamHI BamHI & Sal1 4kb, 5kb BamHI & Pst1 6kb& 3kb Sal1&Pst1 1kb& 8kb kb
Hi I have a problem with number 5, it involves gel
analysis results. There are 2 parts, a,b,c. For A Im sure you need
to make a graph with distance in (cm) on the vertical axis and
log10 bp on the horitzontal. I need help figuring out where to
start and what to do. Please help!
The following question will provide practice in interpreting and analyzing gel results You obtained the DNA electrophoresis gel below. Three samples of lambda phage...
Restriction Mapping Below is a restriction map for the plasmid PGEN101 (total length - 20 kb). Using this map as a guide, give the number of restriction fragments along with their associated lengths that would result from digesting PGEN101 with the restriction enzymes EcoRI, BamHII, anda combination of EcoRI + BamHI. BamHI BamHI BamHI / PGEN101 (20 kb) Mb EcoRI Digest Performed Size Emments Obtained EcoRI........ BamHI.. EcoRI + BamHI.... Two freshmen college students, interested in becoming gene jocks, performed...
Most questions answered within 3 hours.
-
Write a program to solve the Josephus problem, with the following
modification:
Sample Input:
./a.out n...
asked 23 minutes ago -
At the start of a CD it is spinning at a rate of 525 rpm
(revolutions...
asked 59 minutes ago -
4. Without doing any calculations, predict whether the observed
∆T would increase, decrease or remain the...
asked 2 hours ago -
Based on the range, which of the following sets of scores has
the greatest variability? 3,...
asked 3 hours ago -
Ripples in a pond travel at a velocity of 3 m/s with one peak
passing a...
asked 3 hours ago -
A man stands on the roof of a building of height 13.0 mm and
throws a...
asked 3 hours ago -
The extent to which assets are financed by borrowed funds and
other liabilities is indicated by:...
asked 4 hours ago -
Explain in detail
Germany is the fifth largest economy
explain what goods and services Germany specializes...
asked 4 hours ago -
The density of platinum is 21.45 g/mL. If a cube of platinum
with a mass of...
asked 4 hours ago -
Accounts Receivable
Sales
A/R Posting
Extended Sales Invoice
Packing Slip
Compare invoice to packing slip 2...
asked 4 hours ago -
Michaella, age 23, is a full-time law student and is claimed by
her parents as a...
asked 4 hours ago -
Why are polymers not typically casted into products?
asked 5 hours ago