DNA polymerase is involved in firing DNA replication. In prokaryotes there are 3 types of DNA polymerase are present with each different functions and activity.
DNA POLYMERASE III is main enzyme involve in replication, it has 3' - 5' exonuclease activity. This removes bases from 3' end of newly synthsized DNA stand and act as inital or first line defense in generic stability.
DNA polymerase I, this polymerase has unique 5' - 3' exonuclease activity. This enzyme is involved in removal of primers from okazaki fragment in 5' - 3' direction. Thus spced is also filled by DNA polymerase I.
UESTION 3 Explain the importance of exonuclease activity by DNA pol I and III. TT TT...
1. Which of these proteins have 3' to 5' exonuclease activity? (A) DNA pol I (B) DNA pol (C) Primase (D) Topoisomerase at kind of mutation is the replacement of a purine-pyrimidine base pair with a pyrimidine- purine base pair, or vice versa? (A) transition mutation (B) insertion (C) transversion mutation (D) missense mutation 3. Whi (A) Tus proteins (B) oriC (C) Ter sites (D) Primosome 4. Which of the following does NOT involve in translesion synthesis in E. coli?...
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Match the enzyme activity in DNA synthesis with its function. DNA pol III (_) fragments Helicase (_) Primase (_) using template strand DNA pol I (_) double helix DNA ligase (_) adds nucleotides Topoisomerase (_) primer A. anneals DNA B. relieves overwinding C. adds nucleotides D. unwinds parental E. removes RNA primer, F. adds a short RNA 10 Proofreading and repair of the DNA double helix does NOT involves a. detecting a mis-match in base pairs. b. removing the mis-match...
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1. Which of the following statements applies to the 5' - 3' exonuclease activity of DNA polymerase I? You may select multiple answers. a. It allows the enzyme to remove the primer from the primer's 5' end. b. It allows the enzyme to proofread itself. c. It cuts phosphodiester bonds. d. It is used to synthesize a new DNA molecule. e. It is used to seal together Okazaki fragments. 2. Which enzyme are able to proofread themselves? Select all that...
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