The molar ratio of insert DNA to vector DNA used in a ligation is generally about...
The molar ratio of insert DNA to vector DNA used in a ligation is generally about 3:1. Using a vector that is 12 kb and an insert that is 4 kb, how much insert would you use if you used 30 ng of vector? Give your answer in ng and only write the number, do not include the units.
Homework Answers
we use 30ng of insert . We have a formula to calculate required mass of insert.
required mass insert= Insert DNA length/ Vector length X mass of vector X molar ratio (DNA/Vector)
= 4/12 X 30 X 3/1
= 30
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The molar ratio of insert DNA to vector DNA used in a ligation
is generally about...
The molar ratio of insert DNA to vector DNA used in a ligation is generally about...
The molar ratio of insert DNA to vector DNA used in a ligation is generally about 2:1. Using a vector that is 8 kb and an insert that is 4 kb, how much insert would you use if you used 20 ng of vector?
I have no idea what clarification you need the question is as follows I don't understand...
I have no idea what clarification you need the
question is as follows I don't understand how to get the answer
4. For a ligation reaction, it is generally recommended to add Insert to Vector at a molecular ratio of 3:1 (three molecules of insert to one molecule of vector). If your vector and insert preparations have the same DNA concentration (in ng/ul), but your vector is 4500 base pairs long and your insert only 1500 base pairs, which volume...
Does anyone know these biology questions?
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4. A group of students from last year completed ligation under conditions similar to the ones...
4. A group of students from last year completed ligation under conditions similar to the ones you will be using this week. Refer to the gel picture obtained by this group to fill the summary table provided hereafter. You should assume a ligation molar ratio of insert/plasmid of 4:1 and that you will use 30 ng of plasmid for the ligation. A molar ratio of 4:1 means that there should be 4 molecules of insert for every molecule of plasmid....
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i need help with the whole page! 8. You try to ligate a cDNA fragment with...
i
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Example program #include <string> #include <iostream> #include <cmath> #include <vector> using namespace std; vector<int> factor(int n)...
Example program
#include <string>
#include <iostream>
#include <cmath>
#include <vector>
using namespace std;
vector<int> factor(int n)
{
vector <int> v1;
// Print the number of 2s that divide n
while (n%2 == 0)
{
printf("%d ", 2);
n = n/2;
v1.push_back(2);
}
// n must be odd at this point. So we can
skip
// one element (Note i = i +2)
for (int i = 3; i <=...
I have no idea what clarification you need the
question is as follows I don't understand how to get the answer
4. For a ligation reaction, it is generally recommended to add Insert to Vector at a molecular ratio of 3:1 (three molecules of insert to one molecule of vector). If your vector and insert preparations have the same DNA concentration (in ng/ul), but your vector is 4500 base pairs long and your insert only 1500 base pairs, which volume...
4. A group of students from last year completed ligation under conditions similar to the ones you will be using this week. Refer to the gel picture obtained by this group to fill the summary table provided hereafter. You should assume a ligation molar ratio of insert/plasmid of 4:1 and that you will use 30 ng of plasmid for the ligation. A molar ratio of 4:1 means that there should be 4 molecules of insert for every molecule of plasmid....
i
need help with the whole page!
8. You try to ligate a cDNA fragment with Bgl Il and Xho I sticky ends into the Bgl Il and Xho I sites in the multi- cloning site of a vector (vector plus insert: 7 kbp). The plasmid vector has an ampicillin resistance gene. What do you see on the plate next day if you spread the transformed bacteria on an LB agar plate with ampicillin- (1) bacteria with no vector, (2)...
1. What is leverage and how does it work? What is the main concern about using it? 2. Why do we need the quick ratio when we have the current ratio? 3. Why do people view having too much debt as risky? If you were interested in determining whether a company had too much debt, what measure would you use? Why? How much debt do you think would generally be considered too much?
Example program
#include <string>
#include <iostream>
#include <cmath>
#include <vector>
using namespace std;
vector<int> factor(int n)
{
vector <int> v1;
// Print the number of 2s that divide n
while (n%2 == 0)
{
printf("%d ", 2);
n = n/2;
v1.push_back(2);
}
// n must be odd at this point. So we can
skip
// one element (Note i = i +2)
for (int i = 3; i <=...
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